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Dysregulated Free Fatty Acid Receptor 2 Exacerbates Colonic Adenoma Formation in Apc(Min/+) Mice: Relation to Metabolism and Gut Microbiota Composition

Free fatty acid receptor 2 (FFAR2) has been reported as a tumor suppressor in colon cancer development. The current study investigated the effects of FFAR2 signaling on energy metabolism and gut microbiota profiling in a colorectal cancer mouse model (Apc(Min/+)). Ffar2 deficiency promoted colonic p...

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Autores principales: Huang, Yi-Wen, Lin, Chien-Wei, Pan, Pan, Echeveste, Carla Elena, Dong, Athena, Oshima, Kiyoko, Yearsley, Martha, Yu, Jianhua, Wang, Li-Shu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society of Cancer Prevention 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020170/
https://www.ncbi.nlm.nih.gov/pubmed/33842404
http://dx.doi.org/10.15430/JCP.2021.26.1.32
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author Huang, Yi-Wen
Lin, Chien-Wei
Pan, Pan
Echeveste, Carla Elena
Dong, Athena
Oshima, Kiyoko
Yearsley, Martha
Yu, Jianhua
Wang, Li-Shu
author_facet Huang, Yi-Wen
Lin, Chien-Wei
Pan, Pan
Echeveste, Carla Elena
Dong, Athena
Oshima, Kiyoko
Yearsley, Martha
Yu, Jianhua
Wang, Li-Shu
author_sort Huang, Yi-Wen
collection PubMed
description Free fatty acid receptor 2 (FFAR2) has been reported as a tumor suppressor in colon cancer development. The current study investigated the effects of FFAR2 signaling on energy metabolism and gut microbiota profiling in a colorectal cancer mouse model (Apc(Min/+)). Ffar2 deficiency promoted colonic polyp development and enhanced fatty acid oxidation and bile acid metabolism. Gut microbiome sequencing analysis showed distinct clustering among wild-type, Apc(Min/+), and Apc(Min/+)-Ffar2(-/-) mice. The relative abundance of Flavobacteriaceae and Verrucomicrobiaceae was significantly increased in the Apc(Min/+)-Ffar2(-/-) mice compared to the Apc(Min/+) mice. In addition, knocking-down FFAR2 in the human colon cancer cell lines (SW480 and HT29) resulted in increased expression of several key enzymes in fatty acid oxidation, such as carnitine palmitoyltransferase 2, acyl-CoA dehydrogenase, long-chain acyl-CoA dehydrogenase, C-2 to C-3 short chain, and hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase, alpha subunit. Collectively, these results demonstrated that Ffar2 deficiency significantly altered profiles of fatty acid metabolites and gut microbiome, which might promote colorectal cancer development.
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spelling pubmed-80201702021-04-09 Dysregulated Free Fatty Acid Receptor 2 Exacerbates Colonic Adenoma Formation in Apc(Min/+) Mice: Relation to Metabolism and Gut Microbiota Composition Huang, Yi-Wen Lin, Chien-Wei Pan, Pan Echeveste, Carla Elena Dong, Athena Oshima, Kiyoko Yearsley, Martha Yu, Jianhua Wang, Li-Shu J Cancer Prev Original Article Free fatty acid receptor 2 (FFAR2) has been reported as a tumor suppressor in colon cancer development. The current study investigated the effects of FFAR2 signaling on energy metabolism and gut microbiota profiling in a colorectal cancer mouse model (Apc(Min/+)). Ffar2 deficiency promoted colonic polyp development and enhanced fatty acid oxidation and bile acid metabolism. Gut microbiome sequencing analysis showed distinct clustering among wild-type, Apc(Min/+), and Apc(Min/+)-Ffar2(-/-) mice. The relative abundance of Flavobacteriaceae and Verrucomicrobiaceae was significantly increased in the Apc(Min/+)-Ffar2(-/-) mice compared to the Apc(Min/+) mice. In addition, knocking-down FFAR2 in the human colon cancer cell lines (SW480 and HT29) resulted in increased expression of several key enzymes in fatty acid oxidation, such as carnitine palmitoyltransferase 2, acyl-CoA dehydrogenase, long-chain acyl-CoA dehydrogenase, C-2 to C-3 short chain, and hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase, alpha subunit. Collectively, these results demonstrated that Ffar2 deficiency significantly altered profiles of fatty acid metabolites and gut microbiome, which might promote colorectal cancer development. Korean Society of Cancer Prevention 2021-03-30 2021-03-30 /pmc/articles/PMC8020170/ /pubmed/33842404 http://dx.doi.org/10.15430/JCP.2021.26.1.32 Text en Copyright © 2021 Korean Society of Cancer Prevention This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Huang, Yi-Wen
Lin, Chien-Wei
Pan, Pan
Echeveste, Carla Elena
Dong, Athena
Oshima, Kiyoko
Yearsley, Martha
Yu, Jianhua
Wang, Li-Shu
Dysregulated Free Fatty Acid Receptor 2 Exacerbates Colonic Adenoma Formation in Apc(Min/+) Mice: Relation to Metabolism and Gut Microbiota Composition
title Dysregulated Free Fatty Acid Receptor 2 Exacerbates Colonic Adenoma Formation in Apc(Min/+) Mice: Relation to Metabolism and Gut Microbiota Composition
title_full Dysregulated Free Fatty Acid Receptor 2 Exacerbates Colonic Adenoma Formation in Apc(Min/+) Mice: Relation to Metabolism and Gut Microbiota Composition
title_fullStr Dysregulated Free Fatty Acid Receptor 2 Exacerbates Colonic Adenoma Formation in Apc(Min/+) Mice: Relation to Metabolism and Gut Microbiota Composition
title_full_unstemmed Dysregulated Free Fatty Acid Receptor 2 Exacerbates Colonic Adenoma Formation in Apc(Min/+) Mice: Relation to Metabolism and Gut Microbiota Composition
title_short Dysregulated Free Fatty Acid Receptor 2 Exacerbates Colonic Adenoma Formation in Apc(Min/+) Mice: Relation to Metabolism and Gut Microbiota Composition
title_sort dysregulated free fatty acid receptor 2 exacerbates colonic adenoma formation in apc(min/+) mice: relation to metabolism and gut microbiota composition
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8020170/
https://www.ncbi.nlm.nih.gov/pubmed/33842404
http://dx.doi.org/10.15430/JCP.2021.26.1.32
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