Cargando…
miR-96-5p, miR-134-5p, miR-181b-5p and miR-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia—archival samples study
MicroRNAs are involved in various pathologies including cancer. The aim of the study was to assess the level of expression of miR-96-5p, -134-5p, -181b-5p, -200b-3p in FFPE samples of prostate cancer, adjacent cancer-free tissue, and benign prostatic hyperplasia. Samples of 23 FFPE prostate cancer a...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021536/ https://www.ncbi.nlm.nih.gov/pubmed/33331954 http://dx.doi.org/10.1007/s00418-020-01941-2 |
_version_ | 1783674766327021568 |
---|---|
author | Pełka, Kacper Klicka, Klaudia Grzywa, Tomasz M. Gondek, Agata Marczewska, Janina M. Garbicz, Filip Szczepaniak, Kinga Paskal, Wiktor Włodarski, Paweł K. |
author_facet | Pełka, Kacper Klicka, Klaudia Grzywa, Tomasz M. Gondek, Agata Marczewska, Janina M. Garbicz, Filip Szczepaniak, Kinga Paskal, Wiktor Włodarski, Paweł K. |
author_sort | Pełka, Kacper |
collection | PubMed |
description | MicroRNAs are involved in various pathologies including cancer. The aim of the study was to assess the level of expression of miR-96-5p, -134-5p, -181b-5p, -200b-3p in FFPE samples of prostate cancer, adjacent cancer-free tissue, and benign prostatic hyperplasia. Samples of 23 FFPE prostate cancer and 22 benign prostatic hyperplasias were dissected and HE stained. Compartments of tumor tissue and adjacent healthy glandular tissue were isolated from each sample using Laser Capture Microdissection. Total RNA was isolated from dissected tissues. Expression of miR-96-5p, miR-134-5p, 181b-5p, and miR-200b-3p was determined by real-time RT-qPCR method. The expression of miR-200b-3p was significantly higher in cancerous prostate: both in adenocarcinomatous glands and in the adjacent, apparently unaffected glands compared to BPH samples. The expression of miR-181b-5p was lower in in both prostate cancer tissues and adjacent tissue compared to BPH samples. Expression of miR-96-5p and miR-134-5p was lower in prostate cancer tissues compared to BPH. Levels of miR-96-5p, miR-134-5p, and 181b-5p negatively correlated with the Gleason score. Given further studies, miR-96-5p, miR-134-5p and especially miR-200b-3p and miR-181b-5p may differentiate BPH and PC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00418-020-01941-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-8021536 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-80215362021-04-21 miR-96-5p, miR-134-5p, miR-181b-5p and miR-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia—archival samples study Pełka, Kacper Klicka, Klaudia Grzywa, Tomasz M. Gondek, Agata Marczewska, Janina M. Garbicz, Filip Szczepaniak, Kinga Paskal, Wiktor Włodarski, Paweł K. Histochem Cell Biol Original Paper MicroRNAs are involved in various pathologies including cancer. The aim of the study was to assess the level of expression of miR-96-5p, -134-5p, -181b-5p, -200b-3p in FFPE samples of prostate cancer, adjacent cancer-free tissue, and benign prostatic hyperplasia. Samples of 23 FFPE prostate cancer and 22 benign prostatic hyperplasias were dissected and HE stained. Compartments of tumor tissue and adjacent healthy glandular tissue were isolated from each sample using Laser Capture Microdissection. Total RNA was isolated from dissected tissues. Expression of miR-96-5p, miR-134-5p, 181b-5p, and miR-200b-3p was determined by real-time RT-qPCR method. The expression of miR-200b-3p was significantly higher in cancerous prostate: both in adenocarcinomatous glands and in the adjacent, apparently unaffected glands compared to BPH samples. The expression of miR-181b-5p was lower in in both prostate cancer tissues and adjacent tissue compared to BPH samples. Expression of miR-96-5p and miR-134-5p was lower in prostate cancer tissues compared to BPH. Levels of miR-96-5p, miR-134-5p, and 181b-5p negatively correlated with the Gleason score. Given further studies, miR-96-5p, miR-134-5p and especially miR-200b-3p and miR-181b-5p may differentiate BPH and PC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00418-020-01941-2) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2020-12-17 2021 /pmc/articles/PMC8021536/ /pubmed/33331954 http://dx.doi.org/10.1007/s00418-020-01941-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Original Paper Pełka, Kacper Klicka, Klaudia Grzywa, Tomasz M. Gondek, Agata Marczewska, Janina M. Garbicz, Filip Szczepaniak, Kinga Paskal, Wiktor Włodarski, Paweł K. miR-96-5p, miR-134-5p, miR-181b-5p and miR-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia—archival samples study |
title | miR-96-5p, miR-134-5p, miR-181b-5p and miR-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia—archival samples study |
title_full | miR-96-5p, miR-134-5p, miR-181b-5p and miR-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia—archival samples study |
title_fullStr | miR-96-5p, miR-134-5p, miR-181b-5p and miR-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia—archival samples study |
title_full_unstemmed | miR-96-5p, miR-134-5p, miR-181b-5p and miR-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia—archival samples study |
title_short | miR-96-5p, miR-134-5p, miR-181b-5p and miR-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia—archival samples study |
title_sort | mir-96-5p, mir-134-5p, mir-181b-5p and mir-200b-3p heterogenous expression in sites of prostate cancer versus benign prostate hyperplasia—archival samples study |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021536/ https://www.ncbi.nlm.nih.gov/pubmed/33331954 http://dx.doi.org/10.1007/s00418-020-01941-2 |
work_keys_str_mv | AT pełkakacper mir965pmir1345pmir181b5pandmir200b3pheterogenousexpressioninsitesofprostatecancerversusbenignprostatehyperplasiaarchivalsamplesstudy AT klickaklaudia mir965pmir1345pmir181b5pandmir200b3pheterogenousexpressioninsitesofprostatecancerversusbenignprostatehyperplasiaarchivalsamplesstudy AT grzywatomaszm mir965pmir1345pmir181b5pandmir200b3pheterogenousexpressioninsitesofprostatecancerversusbenignprostatehyperplasiaarchivalsamplesstudy AT gondekagata mir965pmir1345pmir181b5pandmir200b3pheterogenousexpressioninsitesofprostatecancerversusbenignprostatehyperplasiaarchivalsamplesstudy AT marczewskajaninam mir965pmir1345pmir181b5pandmir200b3pheterogenousexpressioninsitesofprostatecancerversusbenignprostatehyperplasiaarchivalsamplesstudy AT garbiczfilip mir965pmir1345pmir181b5pandmir200b3pheterogenousexpressioninsitesofprostatecancerversusbenignprostatehyperplasiaarchivalsamplesstudy AT szczepaniakkinga mir965pmir1345pmir181b5pandmir200b3pheterogenousexpressioninsitesofprostatecancerversusbenignprostatehyperplasiaarchivalsamplesstudy AT paskalwiktor mir965pmir1345pmir181b5pandmir200b3pheterogenousexpressioninsitesofprostatecancerversusbenignprostatehyperplasiaarchivalsamplesstudy AT włodarskipawełk mir965pmir1345pmir181b5pandmir200b3pheterogenousexpressioninsitesofprostatecancerversusbenignprostatehyperplasiaarchivalsamplesstudy |