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Tissue engineering of collagen scaffolds crosslinked with plant based polysaccharides

Ideally, a bioscaffold should mimic the characteristics of an extracellular matrix of a living organ of interest. The present study deals with the formation of composite scaffolds of collagen with gum arabic. Collagen was cross-linked with oxidized gum arabic having aldehyde groups to form a porous...

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Autores principales: Rekulapally, Rohit, Udayachandrika, K., Hamlipur, Sirisha, Sasidharan Nair, Anuja, Pal, Biswajit, Singh, Shashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021656/
https://www.ncbi.nlm.nih.gov/pubmed/33604767
http://dx.doi.org/10.1007/s40204-021-00149-4
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author Rekulapally, Rohit
Udayachandrika, K.
Hamlipur, Sirisha
Sasidharan Nair, Anuja
Pal, Biswajit
Singh, Shashi
author_facet Rekulapally, Rohit
Udayachandrika, K.
Hamlipur, Sirisha
Sasidharan Nair, Anuja
Pal, Biswajit
Singh, Shashi
author_sort Rekulapally, Rohit
collection PubMed
description Ideally, a bioscaffold should mimic the characteristics of an extracellular matrix of a living organ of interest. The present study deals with the formation of composite scaffolds of collagen with gum arabic. Collagen was cross-linked with oxidized gum arabic having aldehyde groups to form a porous block. By changing the oxidation level of gum arabic, incorporation of the polysaccharides into the scaffold could be varied resulting in scaffolds with variable polysaccharide to protein content. A series of scaffolds were made by altering collagen concentration and oxidation level of gum arabic. The scaffolds were tested for their physical properties, stability, biocompatibility and ability to support the cell growth. Results implied that variable polysaccharide incorporation into the scaffolds was possible depending on the oxidation level of gum arabic which could influence the swelling behavior. The scaffolds showed non-toxic behavior towards the mesenchymal stem cells and nucleus pulposa cells using viability assay in culture conditions up to 30 days; the growth of cells was seen at all combinations of gels. Nucleus pulposa cells were able to maintain their phenotype in the GACO gels. The studies show that these scaffolds are potential candidates in applications, such as tissue engineering, and can be designed to match the requirement of different cell/tissues as per their ECM.
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spelling pubmed-80216562021-04-21 Tissue engineering of collagen scaffolds crosslinked with plant based polysaccharides Rekulapally, Rohit Udayachandrika, K. Hamlipur, Sirisha Sasidharan Nair, Anuja Pal, Biswajit Singh, Shashi Prog Biomater Original Research Ideally, a bioscaffold should mimic the characteristics of an extracellular matrix of a living organ of interest. The present study deals with the formation of composite scaffolds of collagen with gum arabic. Collagen was cross-linked with oxidized gum arabic having aldehyde groups to form a porous block. By changing the oxidation level of gum arabic, incorporation of the polysaccharides into the scaffold could be varied resulting in scaffolds with variable polysaccharide to protein content. A series of scaffolds were made by altering collagen concentration and oxidation level of gum arabic. The scaffolds were tested for their physical properties, stability, biocompatibility and ability to support the cell growth. Results implied that variable polysaccharide incorporation into the scaffolds was possible depending on the oxidation level of gum arabic which could influence the swelling behavior. The scaffolds showed non-toxic behavior towards the mesenchymal stem cells and nucleus pulposa cells using viability assay in culture conditions up to 30 days; the growth of cells was seen at all combinations of gels. Nucleus pulposa cells were able to maintain their phenotype in the GACO gels. The studies show that these scaffolds are potential candidates in applications, such as tissue engineering, and can be designed to match the requirement of different cell/tissues as per their ECM. Springer Berlin Heidelberg 2021-02-18 /pmc/articles/PMC8021656/ /pubmed/33604767 http://dx.doi.org/10.1007/s40204-021-00149-4 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Research
Rekulapally, Rohit
Udayachandrika, K.
Hamlipur, Sirisha
Sasidharan Nair, Anuja
Pal, Biswajit
Singh, Shashi
Tissue engineering of collagen scaffolds crosslinked with plant based polysaccharides
title Tissue engineering of collagen scaffolds crosslinked with plant based polysaccharides
title_full Tissue engineering of collagen scaffolds crosslinked with plant based polysaccharides
title_fullStr Tissue engineering of collagen scaffolds crosslinked with plant based polysaccharides
title_full_unstemmed Tissue engineering of collagen scaffolds crosslinked with plant based polysaccharides
title_short Tissue engineering of collagen scaffolds crosslinked with plant based polysaccharides
title_sort tissue engineering of collagen scaffolds crosslinked with plant based polysaccharides
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021656/
https://www.ncbi.nlm.nih.gov/pubmed/33604767
http://dx.doi.org/10.1007/s40204-021-00149-4
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