Cargando…

Characteristics of Salmonella From Chinese Native Chicken Breeds Fed on Conventional or Antibiotic-Free Diets

Salmonella is a common food-borne Gram-negative pathogen with multiple serotypes. Pullorum disease, caused by Salmonella Pullorum, seriously threatens the poultry industry. Many previous studies were focused on the epidemiological characteristics of Salmonella infections in conventional antibiotic u...

Descripción completa

Detalles Bibliográficos
Autores principales: Cui, Lulu, Liu, Qingxiao, Jiang, Zhiyu, Song, Yan, Yi, Shoujing, Qiu, Jianhua, Hao, Guijuan, Sun, Shuhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021795/
https://www.ncbi.nlm.nih.gov/pubmed/33834046
http://dx.doi.org/10.3389/fvets.2021.607491
_version_ 1783674807859019776
author Cui, Lulu
Liu, Qingxiao
Jiang, Zhiyu
Song, Yan
Yi, Shoujing
Qiu, Jianhua
Hao, Guijuan
Sun, Shuhong
author_facet Cui, Lulu
Liu, Qingxiao
Jiang, Zhiyu
Song, Yan
Yi, Shoujing
Qiu, Jianhua
Hao, Guijuan
Sun, Shuhong
author_sort Cui, Lulu
collection PubMed
description Salmonella is a common food-borne Gram-negative pathogen with multiple serotypes. Pullorum disease, caused by Salmonella Pullorum, seriously threatens the poultry industry. Many previous studies were focused on the epidemiological characteristics of Salmonella infections in conventional antibiotic use poultry. However, little is known about Salmonella infections in chicken flocks fed on antibiotic-free diets. Herein, we investigated and compared Salmonella infections in three Chinese native breeders fed on antibiotic-free diets, including the Luhua, Langya, and Qingjiaoma chickens, and one conventional breeder, the Bairi chicken, via analyzing 360 dead embryos in 2019. The results showed that the main Salmonella serotypes detected in a total of 155 isolates were S. Pullorum (82.6%) and S. Enteritidis (17.4%). Coinfection with two serotypes of Salmonella was specifically found in Bairi chicken. The sequence type (ST) in S. Pullorum was ST92 (n = 96) and ST2151 (n = 32), whereas only ST11 (n = 27) was found in S. Enteritidis. The Salmonella isolates from three breeder flocks fed on antibiotic-free diets exhibited phenotypic heterogeneity with a great variety of drug resistance spectrum. Most of the isolates among three chicken breeds Luhua (64.9%, 50/77), Langya (60%, 12/20) and Qingjiaoma (58.3%, 7/12) fed on antibiotic-free diets were resistant to only one antibiotic (erythromycin), whereas the rate of resistance to one antibiotic in conventional Bairi chicken isolates was only 4.3% (2/46). The multidrug-resistance rate in Salmonella isolates from layer flocks fed on antibiotic-free diets (20.2%, 22/109) was significantly (P < 0.0001) lower than that from chickens fed on conventional diets (93.5%, 43/46). However, high rate of resistance to erythromycin (97.4%~100%) and streptomycin (26%~41.7%) were also found among three breeder flocks fed on antibiotic-free diets, indicating resistance to these antibiotics likely spread before antibiotic-free feeding in poultry farms. The findings of this study supplement the epidemiological data of salmonellosis and provide an example of the characteristics of Salmonella in the chicken flocks without direct antibiotic selective pressure.
format Online
Article
Text
id pubmed-8021795
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-80217952021-04-07 Characteristics of Salmonella From Chinese Native Chicken Breeds Fed on Conventional or Antibiotic-Free Diets Cui, Lulu Liu, Qingxiao Jiang, Zhiyu Song, Yan Yi, Shoujing Qiu, Jianhua Hao, Guijuan Sun, Shuhong Front Vet Sci Veterinary Science Salmonella is a common food-borne Gram-negative pathogen with multiple serotypes. Pullorum disease, caused by Salmonella Pullorum, seriously threatens the poultry industry. Many previous studies were focused on the epidemiological characteristics of Salmonella infections in conventional antibiotic use poultry. However, little is known about Salmonella infections in chicken flocks fed on antibiotic-free diets. Herein, we investigated and compared Salmonella infections in three Chinese native breeders fed on antibiotic-free diets, including the Luhua, Langya, and Qingjiaoma chickens, and one conventional breeder, the Bairi chicken, via analyzing 360 dead embryos in 2019. The results showed that the main Salmonella serotypes detected in a total of 155 isolates were S. Pullorum (82.6%) and S. Enteritidis (17.4%). Coinfection with two serotypes of Salmonella was specifically found in Bairi chicken. The sequence type (ST) in S. Pullorum was ST92 (n = 96) and ST2151 (n = 32), whereas only ST11 (n = 27) was found in S. Enteritidis. The Salmonella isolates from three breeder flocks fed on antibiotic-free diets exhibited phenotypic heterogeneity with a great variety of drug resistance spectrum. Most of the isolates among three chicken breeds Luhua (64.9%, 50/77), Langya (60%, 12/20) and Qingjiaoma (58.3%, 7/12) fed on antibiotic-free diets were resistant to only one antibiotic (erythromycin), whereas the rate of resistance to one antibiotic in conventional Bairi chicken isolates was only 4.3% (2/46). The multidrug-resistance rate in Salmonella isolates from layer flocks fed on antibiotic-free diets (20.2%, 22/109) was significantly (P < 0.0001) lower than that from chickens fed on conventional diets (93.5%, 43/46). However, high rate of resistance to erythromycin (97.4%~100%) and streptomycin (26%~41.7%) were also found among three breeder flocks fed on antibiotic-free diets, indicating resistance to these antibiotics likely spread before antibiotic-free feeding in poultry farms. The findings of this study supplement the epidemiological data of salmonellosis and provide an example of the characteristics of Salmonella in the chicken flocks without direct antibiotic selective pressure. Frontiers Media S.A. 2021-03-23 /pmc/articles/PMC8021795/ /pubmed/33834046 http://dx.doi.org/10.3389/fvets.2021.607491 Text en Copyright © 2021 Cui, Liu, Jiang, Song, Yi, Qiu, Hao and Sun. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Cui, Lulu
Liu, Qingxiao
Jiang, Zhiyu
Song, Yan
Yi, Shoujing
Qiu, Jianhua
Hao, Guijuan
Sun, Shuhong
Characteristics of Salmonella From Chinese Native Chicken Breeds Fed on Conventional or Antibiotic-Free Diets
title Characteristics of Salmonella From Chinese Native Chicken Breeds Fed on Conventional or Antibiotic-Free Diets
title_full Characteristics of Salmonella From Chinese Native Chicken Breeds Fed on Conventional or Antibiotic-Free Diets
title_fullStr Characteristics of Salmonella From Chinese Native Chicken Breeds Fed on Conventional or Antibiotic-Free Diets
title_full_unstemmed Characteristics of Salmonella From Chinese Native Chicken Breeds Fed on Conventional or Antibiotic-Free Diets
title_short Characteristics of Salmonella From Chinese Native Chicken Breeds Fed on Conventional or Antibiotic-Free Diets
title_sort characteristics of salmonella from chinese native chicken breeds fed on conventional or antibiotic-free diets
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021795/
https://www.ncbi.nlm.nih.gov/pubmed/33834046
http://dx.doi.org/10.3389/fvets.2021.607491
work_keys_str_mv AT cuilulu characteristicsofsalmonellafromchinesenativechickenbreedsfedonconventionalorantibioticfreediets
AT liuqingxiao characteristicsofsalmonellafromchinesenativechickenbreedsfedonconventionalorantibioticfreediets
AT jiangzhiyu characteristicsofsalmonellafromchinesenativechickenbreedsfedonconventionalorantibioticfreediets
AT songyan characteristicsofsalmonellafromchinesenativechickenbreedsfedonconventionalorantibioticfreediets
AT yishoujing characteristicsofsalmonellafromchinesenativechickenbreedsfedonconventionalorantibioticfreediets
AT qiujianhua characteristicsofsalmonellafromchinesenativechickenbreedsfedonconventionalorantibioticfreediets
AT haoguijuan characteristicsofsalmonellafromchinesenativechickenbreedsfedonconventionalorantibioticfreediets
AT sunshuhong characteristicsofsalmonellafromchinesenativechickenbreedsfedonconventionalorantibioticfreediets