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Theaflavin-3,3′-Digallate Promotes the Formation of Osteoblasts Under Inflammatory Environment and Increases the Bone Mass of Ovariectomized Mice

Postmenopausal osteoporosis is a disease of bone mass reduction and structural changes due to estrogen deficiency, which can eventually lead to increased pain and fracture risk. Chronic inflammatory microenvironment leading to the decreased activation of osteoblasts and inhibition of bone formation...

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Autores principales: Ge, Gaoran, Yang, Sen, Hou, Zhenyang, Gan, Minfeng, Tao, Huaqiang, Zhang, Wei, Li, Wenming, Wang, Zheng, Hao, Yuefeng, Gu, Ye, Geng, Dechun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021853/
https://www.ncbi.nlm.nih.gov/pubmed/33833684
http://dx.doi.org/10.3389/fphar.2021.648969
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author Ge, Gaoran
Yang, Sen
Hou, Zhenyang
Gan, Minfeng
Tao, Huaqiang
Zhang, Wei
Li, Wenming
Wang, Zheng
Hao, Yuefeng
Gu, Ye
Geng, Dechun
author_facet Ge, Gaoran
Yang, Sen
Hou, Zhenyang
Gan, Minfeng
Tao, Huaqiang
Zhang, Wei
Li, Wenming
Wang, Zheng
Hao, Yuefeng
Gu, Ye
Geng, Dechun
author_sort Ge, Gaoran
collection PubMed
description Postmenopausal osteoporosis is a disease of bone mass reduction and structural changes due to estrogen deficiency, which can eventually lead to increased pain and fracture risk. Chronic inflammatory microenvironment leading to the decreased activation of osteoblasts and inhibition of bone formation is an important pathological factor that leads to osteoporosis. Theaflavin-3,3′-digallate (TFDG) is an extract of black tea, which has potential anti-inflammatory and antiviral effects. In our study, we found that TFDG significantly increased the bone mass of ovariectomized (OVX) mice by micro-CT analysis. Compared with OVX mice, TFDG reduced the release of proinflammatory cytokines and increased the expression of osteogenic markers in vivo. In vitro experiments demonstrated that TFDG could promote the formation of osteoblasts in inflammatory environment and enhance their mineralization ability. In this process, TFDG activated MAPK, Wnt/β-Catenin and BMP/Smad signaling pathways inhibited by TNF-α, and then promoted the transcription of osteogenic related factors including Runx2 and Osterix, promoting the differentiation and maturation of osteoblasts eventually. In general, our study confirmed that TFDG was able to promote osteoblast differentiation under inflammatory environment, enhance its mineralization ability, and ultimately increase bone mass in ovariectomized mice. These results suggested that TFDG might have the potential to be a more effective treatment of postmenopausal osteoporosis.
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spelling pubmed-80218532021-04-07 Theaflavin-3,3′-Digallate Promotes the Formation of Osteoblasts Under Inflammatory Environment and Increases the Bone Mass of Ovariectomized Mice Ge, Gaoran Yang, Sen Hou, Zhenyang Gan, Minfeng Tao, Huaqiang Zhang, Wei Li, Wenming Wang, Zheng Hao, Yuefeng Gu, Ye Geng, Dechun Front Pharmacol Pharmacology Postmenopausal osteoporosis is a disease of bone mass reduction and structural changes due to estrogen deficiency, which can eventually lead to increased pain and fracture risk. Chronic inflammatory microenvironment leading to the decreased activation of osteoblasts and inhibition of bone formation is an important pathological factor that leads to osteoporosis. Theaflavin-3,3′-digallate (TFDG) is an extract of black tea, which has potential anti-inflammatory and antiviral effects. In our study, we found that TFDG significantly increased the bone mass of ovariectomized (OVX) mice by micro-CT analysis. Compared with OVX mice, TFDG reduced the release of proinflammatory cytokines and increased the expression of osteogenic markers in vivo. In vitro experiments demonstrated that TFDG could promote the formation of osteoblasts in inflammatory environment and enhance their mineralization ability. In this process, TFDG activated MAPK, Wnt/β-Catenin and BMP/Smad signaling pathways inhibited by TNF-α, and then promoted the transcription of osteogenic related factors including Runx2 and Osterix, promoting the differentiation and maturation of osteoblasts eventually. In general, our study confirmed that TFDG was able to promote osteoblast differentiation under inflammatory environment, enhance its mineralization ability, and ultimately increase bone mass in ovariectomized mice. These results suggested that TFDG might have the potential to be a more effective treatment of postmenopausal osteoporosis. Frontiers Media S.A. 2021-03-23 /pmc/articles/PMC8021853/ /pubmed/33833684 http://dx.doi.org/10.3389/fphar.2021.648969 Text en Copyright © 2021 Ge, Yang, Hou, Gan, Tao, Zhang, Li, Wang, Hao, Gu and Geng. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Ge, Gaoran
Yang, Sen
Hou, Zhenyang
Gan, Minfeng
Tao, Huaqiang
Zhang, Wei
Li, Wenming
Wang, Zheng
Hao, Yuefeng
Gu, Ye
Geng, Dechun
Theaflavin-3,3′-Digallate Promotes the Formation of Osteoblasts Under Inflammatory Environment and Increases the Bone Mass of Ovariectomized Mice
title Theaflavin-3,3′-Digallate Promotes the Formation of Osteoblasts Under Inflammatory Environment and Increases the Bone Mass of Ovariectomized Mice
title_full Theaflavin-3,3′-Digallate Promotes the Formation of Osteoblasts Under Inflammatory Environment and Increases the Bone Mass of Ovariectomized Mice
title_fullStr Theaflavin-3,3′-Digallate Promotes the Formation of Osteoblasts Under Inflammatory Environment and Increases the Bone Mass of Ovariectomized Mice
title_full_unstemmed Theaflavin-3,3′-Digallate Promotes the Formation of Osteoblasts Under Inflammatory Environment and Increases the Bone Mass of Ovariectomized Mice
title_short Theaflavin-3,3′-Digallate Promotes the Formation of Osteoblasts Under Inflammatory Environment and Increases the Bone Mass of Ovariectomized Mice
title_sort theaflavin-3,3′-digallate promotes the formation of osteoblasts under inflammatory environment and increases the bone mass of ovariectomized mice
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021853/
https://www.ncbi.nlm.nih.gov/pubmed/33833684
http://dx.doi.org/10.3389/fphar.2021.648969
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