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Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus
In cystic fibrosis (CF) infectious and allergic airway inflammation cause pulmonary exacerbations that destroy the lungs. Staphylococcus aureus is a common long-term colonizer and cause of recurrent airway infections in CF. The pathogen is also associated with respiratory allergy; especially the sta...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021911/ https://www.ncbi.nlm.nih.gov/pubmed/33833764 http://dx.doi.org/10.3389/fimmu.2021.651060 |
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author | Nordengrün, Maria Abdurrahman, Goran Treffon, Janina Wächter, Hannah Kahl, Barbara C. Bröker, Barbara M. |
author_facet | Nordengrün, Maria Abdurrahman, Goran Treffon, Janina Wächter, Hannah Kahl, Barbara C. Bröker, Barbara M. |
author_sort | Nordengrün, Maria |
collection | PubMed |
description | In cystic fibrosis (CF) infectious and allergic airway inflammation cause pulmonary exacerbations that destroy the lungs. Staphylococcus aureus is a common long-term colonizer and cause of recurrent airway infections in CF. The pathogen is also associated with respiratory allergy; especially the staphylococcal serine protease-like proteins (Spls) can induce type 2 immune responses in humans and mice. We measured the serum IgE levels specific to 7 proteases of S. aureus by ELISA, targeting 5 Spls (76 CF patients and 46 controls) and the staphopains A and B (16 CF patients and 46 controls). Then we compared cytokine release and phenotype of T cells that had been stimulated with Spls between 5 CF patients and 5 controls. CF patients had strongly increased serum IgE binding to all Spls but not to the staphopains. Compared to healthy controls, their Spl-stimulated T cells released more type 2 cytokines (IL-4, IL-5, IL-13) and more IL-6 with no difference in the secretion of type 1- or type 3 cytokines (IFNγ, IL-17A, IL-17F). IL-10 production was low in CF T cells. The phenotype of the Spl-exposed T cells shifted towards a Th2 or Th17 profile in CF but to a Th1 profile in controls. Sensitization to S. aureus Spls is common in CF. This discovery could explain episodes of allergic inflammation of hitherto unknown causation in CF and extend the diagnostic and therapeutic portfolio. |
format | Online Article Text |
id | pubmed-8021911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80219112021-04-07 Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus Nordengrün, Maria Abdurrahman, Goran Treffon, Janina Wächter, Hannah Kahl, Barbara C. Bröker, Barbara M. Front Immunol Immunology In cystic fibrosis (CF) infectious and allergic airway inflammation cause pulmonary exacerbations that destroy the lungs. Staphylococcus aureus is a common long-term colonizer and cause of recurrent airway infections in CF. The pathogen is also associated with respiratory allergy; especially the staphylococcal serine protease-like proteins (Spls) can induce type 2 immune responses in humans and mice. We measured the serum IgE levels specific to 7 proteases of S. aureus by ELISA, targeting 5 Spls (76 CF patients and 46 controls) and the staphopains A and B (16 CF patients and 46 controls). Then we compared cytokine release and phenotype of T cells that had been stimulated with Spls between 5 CF patients and 5 controls. CF patients had strongly increased serum IgE binding to all Spls but not to the staphopains. Compared to healthy controls, their Spl-stimulated T cells released more type 2 cytokines (IL-4, IL-5, IL-13) and more IL-6 with no difference in the secretion of type 1- or type 3 cytokines (IFNγ, IL-17A, IL-17F). IL-10 production was low in CF T cells. The phenotype of the Spl-exposed T cells shifted towards a Th2 or Th17 profile in CF but to a Th1 profile in controls. Sensitization to S. aureus Spls is common in CF. This discovery could explain episodes of allergic inflammation of hitherto unknown causation in CF and extend the diagnostic and therapeutic portfolio. Frontiers Media S.A. 2021-03-23 /pmc/articles/PMC8021911/ /pubmed/33833764 http://dx.doi.org/10.3389/fimmu.2021.651060 Text en Copyright © 2021 Nordengrün, Abdurrahman, Treffon, Wächter, Kahl and Bröker http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Nordengrün, Maria Abdurrahman, Goran Treffon, Janina Wächter, Hannah Kahl, Barbara C. Bröker, Barbara M. Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus |
title | Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus |
title_full | Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus |
title_fullStr | Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus |
title_full_unstemmed | Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus |
title_short | Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus |
title_sort | allergic reactions to serine protease-like proteins of staphylococcus aureus |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021911/ https://www.ncbi.nlm.nih.gov/pubmed/33833764 http://dx.doi.org/10.3389/fimmu.2021.651060 |
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