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Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus

In cystic fibrosis (CF) infectious and allergic airway inflammation cause pulmonary exacerbations that destroy the lungs. Staphylococcus aureus is a common long-term colonizer and cause of recurrent airway infections in CF. The pathogen is also associated with respiratory allergy; especially the sta...

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Autores principales: Nordengrün, Maria, Abdurrahman, Goran, Treffon, Janina, Wächter, Hannah, Kahl, Barbara C., Bröker, Barbara M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021911/
https://www.ncbi.nlm.nih.gov/pubmed/33833764
http://dx.doi.org/10.3389/fimmu.2021.651060
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author Nordengrün, Maria
Abdurrahman, Goran
Treffon, Janina
Wächter, Hannah
Kahl, Barbara C.
Bröker, Barbara M.
author_facet Nordengrün, Maria
Abdurrahman, Goran
Treffon, Janina
Wächter, Hannah
Kahl, Barbara C.
Bröker, Barbara M.
author_sort Nordengrün, Maria
collection PubMed
description In cystic fibrosis (CF) infectious and allergic airway inflammation cause pulmonary exacerbations that destroy the lungs. Staphylococcus aureus is a common long-term colonizer and cause of recurrent airway infections in CF. The pathogen is also associated with respiratory allergy; especially the staphylococcal serine protease-like proteins (Spls) can induce type 2 immune responses in humans and mice. We measured the serum IgE levels specific to 7 proteases of S. aureus by ELISA, targeting 5 Spls (76 CF patients and 46 controls) and the staphopains A and B (16 CF patients and 46 controls). Then we compared cytokine release and phenotype of T cells that had been stimulated with Spls between 5 CF patients and 5 controls. CF patients had strongly increased serum IgE binding to all Spls but not to the staphopains. Compared to healthy controls, their Spl-stimulated T cells released more type 2 cytokines (IL-4, IL-5, IL-13) and more IL-6 with no difference in the secretion of type 1- or type 3 cytokines (IFNγ, IL-17A, IL-17F). IL-10 production was low in CF T cells. The phenotype of the Spl-exposed T cells shifted towards a Th2 or Th17 profile in CF but to a Th1 profile in controls. Sensitization to S. aureus Spls is common in CF. This discovery could explain episodes of allergic inflammation of hitherto unknown causation in CF and extend the diagnostic and therapeutic portfolio.
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spelling pubmed-80219112021-04-07 Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus Nordengrün, Maria Abdurrahman, Goran Treffon, Janina Wächter, Hannah Kahl, Barbara C. Bröker, Barbara M. Front Immunol Immunology In cystic fibrosis (CF) infectious and allergic airway inflammation cause pulmonary exacerbations that destroy the lungs. Staphylococcus aureus is a common long-term colonizer and cause of recurrent airway infections in CF. The pathogen is also associated with respiratory allergy; especially the staphylococcal serine protease-like proteins (Spls) can induce type 2 immune responses in humans and mice. We measured the serum IgE levels specific to 7 proteases of S. aureus by ELISA, targeting 5 Spls (76 CF patients and 46 controls) and the staphopains A and B (16 CF patients and 46 controls). Then we compared cytokine release and phenotype of T cells that had been stimulated with Spls between 5 CF patients and 5 controls. CF patients had strongly increased serum IgE binding to all Spls but not to the staphopains. Compared to healthy controls, their Spl-stimulated T cells released more type 2 cytokines (IL-4, IL-5, IL-13) and more IL-6 with no difference in the secretion of type 1- or type 3 cytokines (IFNγ, IL-17A, IL-17F). IL-10 production was low in CF T cells. The phenotype of the Spl-exposed T cells shifted towards a Th2 or Th17 profile in CF but to a Th1 profile in controls. Sensitization to S. aureus Spls is common in CF. This discovery could explain episodes of allergic inflammation of hitherto unknown causation in CF and extend the diagnostic and therapeutic portfolio. Frontiers Media S.A. 2021-03-23 /pmc/articles/PMC8021911/ /pubmed/33833764 http://dx.doi.org/10.3389/fimmu.2021.651060 Text en Copyright © 2021 Nordengrün, Abdurrahman, Treffon, Wächter, Kahl and Bröker http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Nordengrün, Maria
Abdurrahman, Goran
Treffon, Janina
Wächter, Hannah
Kahl, Barbara C.
Bröker, Barbara M.
Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus
title Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus
title_full Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus
title_fullStr Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus
title_full_unstemmed Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus
title_short Allergic Reactions to Serine Protease-Like Proteins of Staphylococcus aureus
title_sort allergic reactions to serine protease-like proteins of staphylococcus aureus
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8021911/
https://www.ncbi.nlm.nih.gov/pubmed/33833764
http://dx.doi.org/10.3389/fimmu.2021.651060
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