An update about molecular biology techniques to detect orthopaedic implant-related infections

Despite different criteria to diagnose a prosthetic joint infection (PJI), aetiological diagnosis of the causing microorganism remains essential to guide treatment. Molecular-biology-based PJI diagnosis is progressing (faster, higher specificity) in different techniques, from the experimental labora...

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Detalles Bibliográficos
Autores principales: Esteban, Jaime, Gómez-Barrena, Enrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: British Editorial Society of Bone and Joint Surgery 2021
Materias:
General Orthopaedics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022009/
https://www.ncbi.nlm.nih.gov/pubmed/33828851
http://dx.doi.org/10.1302/2058-5241.6.200118
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author Esteban, Jaime
Gómez-Barrena, Enrique
author_facet Esteban, Jaime
Gómez-Barrena, Enrique
author_sort Esteban, Jaime
collection PubMed
description Despite different criteria to diagnose a prosthetic joint infection (PJI), aetiological diagnosis of the causing microorganism remains essential to guide treatment. Molecular-biology-based PJI diagnosis is progressing (faster, higher specificity) in different techniques, from the experimental laboratory into clinical use. Multiplex polymerase chain reaction techniques (custom-made or commercial) provide satisfactory results in clinical series of cases, with specificity close to 100% and sensitivity over 70–80%. Next-generation metagenomics may increase sensitivity while maintaining high specificity. Molecular biology techniques may represent, in the next five years, a significant transformation of the currently available microbiological diagnosis in PJI. Cite this article: EFORT Open Rev 2021;6:93-100. DOI: 10.1302/2058-5241.6.200118
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spelling pubmed-80220092021-04-06 An update about molecular biology techniques to detect orthopaedic implant-related infections Esteban, Jaime Gómez-Barrena, Enrique EFORT Open Rev General Orthopaedics Despite different criteria to diagnose a prosthetic joint infection (PJI), aetiological diagnosis of the causing microorganism remains essential to guide treatment. Molecular-biology-based PJI diagnosis is progressing (faster, higher specificity) in different techniques, from the experimental laboratory into clinical use. Multiplex polymerase chain reaction techniques (custom-made or commercial) provide satisfactory results in clinical series of cases, with specificity close to 100% and sensitivity over 70–80%. Next-generation metagenomics may increase sensitivity while maintaining high specificity. Molecular biology techniques may represent, in the next five years, a significant transformation of the currently available microbiological diagnosis in PJI. Cite this article: EFORT Open Rev 2021;6:93-100. DOI: 10.1302/2058-5241.6.200118 British Editorial Society of Bone and Joint Surgery 2021-02-01 /pmc/articles/PMC8022009/ /pubmed/33828851 http://dx.doi.org/10.1302/2058-5241.6.200118 Text en © 2021 The author(s) https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-Non Commercial 4.0 International (CC BY-NC 4.0) licence (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed.
spellingShingle General Orthopaedics
Esteban, Jaime
Gómez-Barrena, Enrique
An update about molecular biology techniques to detect orthopaedic implant-related infections
title An update about molecular biology techniques to detect orthopaedic implant-related infections
title_full An update about molecular biology techniques to detect orthopaedic implant-related infections
title_fullStr An update about molecular biology techniques to detect orthopaedic implant-related infections
title_full_unstemmed An update about molecular biology techniques to detect orthopaedic implant-related infections
title_short An update about molecular biology techniques to detect orthopaedic implant-related infections
title_sort update about molecular biology techniques to detect orthopaedic implant-related infections
topic General Orthopaedics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022009/
https://www.ncbi.nlm.nih.gov/pubmed/33828851
http://dx.doi.org/10.1302/2058-5241.6.200118
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