An RNA-interference screen in Drosophila to identify ZAD-containing C2H2 zinc finger genes that function in female germ cells

The zinc finger-associated domain (ZAD) is present in over 90 C2H2 zinc finger (ZNF) proteins. Despite their abundance, only a few ZAD-ZNF genes have been characterized to date. Here, we systematically analyze the function of 68 ZAD-ZNF genes in Drosophila female germ cells by performing an in vivo...

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Autores principales: Shapiro-Kulnane, Laura, Bautista, Oscar, Salz, Helen K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Mutant Screen Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022714/
https://www.ncbi.nlm.nih.gov/pubmed/33561227
http://dx.doi.org/10.1093/g3journal/jkaa016
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author Shapiro-Kulnane, Laura
Bautista, Oscar
Salz, Helen K
author_facet Shapiro-Kulnane, Laura
Bautista, Oscar
Salz, Helen K
author_sort Shapiro-Kulnane, Laura
collection PubMed
description The zinc finger-associated domain (ZAD) is present in over 90 C2H2 zinc finger (ZNF) proteins. Despite their abundance, only a few ZAD-ZNF genes have been characterized to date. Here, we systematically analyze the function of 68 ZAD-ZNF genes in Drosophila female germ cells by performing an in vivo RNA-interference screen. We identified eight ZAD-ZNF genes required for oogenesis, and based on further characterization of the knockdown phenotypes, we uncovered defects broadly consistent with functions in germ cell specification and/or survival, early differentiation, and egg chamber maturation. These results provide a candidate pool for future studies aimed at functionalization of this large but poorly characterized gene family.
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spelling pubmed-80227142021-04-09 An RNA-interference screen in Drosophila to identify ZAD-containing C2H2 zinc finger genes that function in female germ cells Shapiro-Kulnane, Laura Bautista, Oscar Salz, Helen K G3 (Bethesda) Mutant Screen Report The zinc finger-associated domain (ZAD) is present in over 90 C2H2 zinc finger (ZNF) proteins. Despite their abundance, only a few ZAD-ZNF genes have been characterized to date. Here, we systematically analyze the function of 68 ZAD-ZNF genes in Drosophila female germ cells by performing an in vivo RNA-interference screen. We identified eight ZAD-ZNF genes required for oogenesis, and based on further characterization of the knockdown phenotypes, we uncovered defects broadly consistent with functions in germ cell specification and/or survival, early differentiation, and egg chamber maturation. These results provide a candidate pool for future studies aimed at functionalization of this large but poorly characterized gene family. Oxford University Press 2020-12-07 /pmc/articles/PMC8022714/ /pubmed/33561227 http://dx.doi.org/10.1093/g3journal/jkaa016 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Mutant Screen Report
Shapiro-Kulnane, Laura
Bautista, Oscar
Salz, Helen K
An RNA-interference screen in Drosophila to identify ZAD-containing C2H2 zinc finger genes that function in female germ cells
title An RNA-interference screen in Drosophila to identify ZAD-containing C2H2 zinc finger genes that function in female germ cells
title_full An RNA-interference screen in Drosophila to identify ZAD-containing C2H2 zinc finger genes that function in female germ cells
title_fullStr An RNA-interference screen in Drosophila to identify ZAD-containing C2H2 zinc finger genes that function in female germ cells
title_full_unstemmed An RNA-interference screen in Drosophila to identify ZAD-containing C2H2 zinc finger genes that function in female germ cells
title_short An RNA-interference screen in Drosophila to identify ZAD-containing C2H2 zinc finger genes that function in female germ cells
title_sort rna-interference screen in drosophila to identify zad-containing c2h2 zinc finger genes that function in female germ cells
topic Mutant Screen Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022714/
https://www.ncbi.nlm.nih.gov/pubmed/33561227
http://dx.doi.org/10.1093/g3journal/jkaa016
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