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Evolution of Chi motifs in Proteobacteria
Homologous recombination is a key pathway found in nearly all bacterial taxa. The recombination complex not only allows bacteria to repair DNA double-strand breaks but also promotes adaption through the exchange of DNA between cells. In Proteobacteria, this process is mediated by the RecBCD complex,...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022716/ https://www.ncbi.nlm.nih.gov/pubmed/33561247 http://dx.doi.org/10.1093/g3journal/jkaa054 |
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author | Buton, Angélique Bobay, Louis-Marie |
author_facet | Buton, Angélique Bobay, Louis-Marie |
author_sort | Buton, Angélique |
collection | PubMed |
description | Homologous recombination is a key pathway found in nearly all bacterial taxa. The recombination complex not only allows bacteria to repair DNA double-strand breaks but also promotes adaption through the exchange of DNA between cells. In Proteobacteria, this process is mediated by the RecBCD complex, which relies on the recognition of a DNA motif named Chi to initiate recombination. The Chi motif has been characterized in Escherichia coli and analogous sequences have been found in several other species from diverse families, suggesting that this mode of action is widespread across bacteria. However, the sequences of Chi-like motifs are known for only five bacterial species: E. coli, Haemophilus influenzae, Bacillus subtilis, Lactococcus lactis, and Staphylococcus aureus. In this study, we detected putative Chi motifs in a large dataset of Proteobacteria and identified four additional motifs sharing high sequence similarity and similar properties to the Chi motif of E. coli in 85 species of Proteobacteria. Most Chi motifs were detected in Enterobacteriaceae and this motif appears well conserved in this family. However, we did not detect Chi motifs for the majority of Proteobacteria, suggesting that different motifs are used in these species. Altogether these results substantially expand our knowledge on the evolution of Chi motifs and on the recombination process in bacteria. |
format | Online Article Text |
id | pubmed-8022716 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-80227162021-04-09 Evolution of Chi motifs in Proteobacteria Buton, Angélique Bobay, Louis-Marie G3 (Bethesda) Investigation Homologous recombination is a key pathway found in nearly all bacterial taxa. The recombination complex not only allows bacteria to repair DNA double-strand breaks but also promotes adaption through the exchange of DNA between cells. In Proteobacteria, this process is mediated by the RecBCD complex, which relies on the recognition of a DNA motif named Chi to initiate recombination. The Chi motif has been characterized in Escherichia coli and analogous sequences have been found in several other species from diverse families, suggesting that this mode of action is widespread across bacteria. However, the sequences of Chi-like motifs are known for only five bacterial species: E. coli, Haemophilus influenzae, Bacillus subtilis, Lactococcus lactis, and Staphylococcus aureus. In this study, we detected putative Chi motifs in a large dataset of Proteobacteria and identified four additional motifs sharing high sequence similarity and similar properties to the Chi motif of E. coli in 85 species of Proteobacteria. Most Chi motifs were detected in Enterobacteriaceae and this motif appears well conserved in this family. However, we did not detect Chi motifs for the majority of Proteobacteria, suggesting that different motifs are used in these species. Altogether these results substantially expand our knowledge on the evolution of Chi motifs and on the recombination process in bacteria. Oxford University Press 2021-01-05 /pmc/articles/PMC8022716/ /pubmed/33561247 http://dx.doi.org/10.1093/g3journal/jkaa054 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Investigation Buton, Angélique Bobay, Louis-Marie Evolution of Chi motifs in Proteobacteria |
title | Evolution of Chi motifs in Proteobacteria |
title_full | Evolution of Chi motifs in Proteobacteria |
title_fullStr | Evolution of Chi motifs in Proteobacteria |
title_full_unstemmed | Evolution of Chi motifs in Proteobacteria |
title_short | Evolution of Chi motifs in Proteobacteria |
title_sort | evolution of chi motifs in proteobacteria |
topic | Investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022716/ https://www.ncbi.nlm.nih.gov/pubmed/33561247 http://dx.doi.org/10.1093/g3journal/jkaa054 |
work_keys_str_mv | AT butonangelique evolutionofchimotifsinproteobacteria AT bobaylouismarie evolutionofchimotifsinproteobacteria |