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A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience
Undergraduate students participating in the UCLA Undergraduate Research Consortium for Functional Genomics (URCFG) have conducted a two-phased screen using RNA interference (RNAi) in combination with fluorescent reporter proteins to identify genes important for hematopoiesis in Drosophila. This scre...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022729/ https://www.ncbi.nlm.nih.gov/pubmed/33561251 http://dx.doi.org/10.1093/g3journal/jkaa028 |
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author | Evans, Cory J Olson, John M Mondal, Bama Charan Kandimalla, Pratyush Abbasi, Ariano Abdusamad, Mai M Acosta, Osvaldo Ainsworth, Julia A Akram, Haris M Albert, Ralph B Alegria-Leal, Elitzander Alexander, Kai Y Ayala, Angelica C Balashova, Nataliya S Barber, Rebecca M Bassi, Harmanjit Bennion, Sean P Beyder, Miriam Bhatt, Kush V Bhoot, Chinmay Bradshaw, Aaron W Brannigan, Tierney G Cao, Boyu Cashell, Yancey Y Chai, Timothy Chan, Alex W Chan, Carissa Chang, Inho Chang, Jonathan Chang, Michael T Chang, Patrick W Chang, Stephen Chari, Neel Chassiakos, Alexander J Chen, Iris E Chen, Vivian K Chen, Zheying Cheng, Marsha R Chiang, Mimi Chiu, Vivian Choi, Sharon Chung, Jun Ho Contreras, Liset Corona, Edgar Cruz, Courtney J Cruz, Renae L Dang, Jefferson M Dasari, Suhas P De La Fuente, Justin R O Del Rio, Oscar M A Dennis, Emily R Dertsakyan, Petros S Dey, Ipsita Distler, Rachel S Dong, Zhiqiao Dorman, Leah C Douglass, Mark A Ehresman, Allysen B Fu, Ivy H Fua, Andrea Full, Sean M Ghaffari-Rafi, Arash Ghani, Asmar Abdul Giap, Bosco Gill, Sonia Gill, Zafar S Gills, Nicholas J Godavarthi, Sindhuja Golnazarian, Talin Goyal, Raghav Gray, Ricardo Grunfeld, Alexander M Gu, Kelly M Gutierrez, Natalia C Ha, An N Hamid, Iman Hanson, Ashley Hao, Celesti He, Chongbin He, Mengshi Hedtke, Joshua P Hernandez, Ysrael K Hlaing, Hnin Hobby, Faith A Hoi, Karen Hope, Ashley C Hosseinian, Sahra M Hsu, Alice Hsueh, Jennifer Hu, Eileen Hu, Spencer S Huang, Stephanie Huang, Wilson Huynh, Melanie Javier, Carmen Jeon, Na Eun Ji, Sunjong Johal, Jasmin John, Amala Johnson, Lauren Kadakia, Saurin Kakade, Namrata Kamel, Sarah Kaur, Ravinder Khatra, Jagteshwar S Kho, Jeffrey A Kim, Caleb Kim, Emily Jin-Kyung Kim, Hee Jong Kim, Hyun Wook Kim, Jin Hee Kim, Seong Ah Kim, Woo Kyeom Kit, Brian La, Cindy Lai, Jonathan Lam, Vivian Le, Nguyen Khoi Lee, Chi Ju Lee, Dana Lee, Dong Yeon Lee, James Lee, Jason Lee, Jessica Lee, Ju-Yeon Lee, Sharon Lee, Terrence C Lee, Victoria Li, Amber J Li, Jialing Libro, Alexandra M Lien, Irvin C Lim, Mia Lin, Jeffrey M Liu, Connie Y Liu, Steven C Louie, Irene Lu, Shijia W Luo, William Y Luu, Tiffany Madrigal, Josef T Mai, Yishan Miya, Darron I Mohammadi, Mina Mohanta, Sayonika Mokwena, Tebogo Montoya, Tonatiuh Mould, Dallas L Murata, Mark R Muthaiya, Janani Naicker, Seethim Neebe, Mallory R Ngo, Amy Ngo, Duy Q Ngo, Jamie A Nguyen, Anh T Nguyen, Huy C X Nguyen, Rina H Nguyen, Thao T T Nguyen, Vincent T Nishida, Kevin Oh, Seo-Kyung Omi, Kristen M Onglatco, Mary C Almazan, Guadalupe Ortega Paguntalan, Jahzeel Panchal, Maharshi Pang, Stephanie Parikh, Harin B Patel, Purvi D Patel, Trisha H Petersen, Julia E Pham, Steven Phan-Everson, Tien M Pokhriyal, Megha Popovich, Davis W Quaal, Adam T Querubin, Karl Resendiz, Anabel Riabkova, Nadezhda Rong, Fred Salarkia, Sarah Sama, Nateli Sang, Elaine Sanville, David A Schoen, Emily R Shen, Zhouyang Siangchin, Ken Sibal, Gabrielle Sin, Garuem Sjarif, Jasmine Smith, Christopher J Soeboer, Annisa N Sosa, Cristian Spitters, Derek Stender, Bryan Su, Chloe C Summapund, Jenny Sun, Beatrice J Sutanto, Christine Tan, Jaime S Tan, Nguon L Tangmatitam, Parich Trac, Cindy K Tran, Conny Tran, Daniel Tran, Duy Tran, Vina Truong, Patrick A Tsai, Brandon L Tsai, Pei-Hua Tsui, C Kimberly Uriu, Jackson K Venkatesh, Sanan Vo, Maique Vo, Nhat-Thi Vo, Phuong Voros, Timothy C Wan, Yuan Wang, Eric Wang, Jeffrey Wang, Michael K Wang, Yuxuan Wei, Siman Wilson, Matthew N Wong, Daniel Wu, Elliott Xing, Hanning Xu, Jason P Yaftaly, Sahar Yan, Kimberly Yang, Evan Yang, Rebecca Yao, Tony Yeo, Patricia Yip, Vivian Yogi, Puja Young, Gloria Chin Yung, Maggie M Zai, Alexander Zhang, Christine Zhang, Xiao X Zhao, Zijun Zhou, Raymond Zhou, Ziqi Abutouk, Mona Aguirre, Brian Ao, Chon Baranoff, Alexis Beniwal, Angad Cai, Zijie Chan, Ryan Chien, Kenneth Chang Chaudhary, Umar Chin, Patrick Chowdhury, Praptee Dalie, Jamlah Du, Eric Y Estrada, Alec Feng, Erwin Ghaly, Monica Graf, Rose Hernandez, Eduardo Herrera, Kevin Ho, Vivien W Honeychurch, Kaitlyn Hou, Yurianna Huang, Jo M Ishii, Momoko James, Nicholas Jang, Gah-Eun Jin, Daphne Juarez, Jesse Kesaf, Ayse Elif Khalsa, Sat Kartar Kim, Hannah Kovsky, Jenna Kuang, Chak Lon Kumar, Shraddha Lam, Gloria Lee, Ceejay Lee, Grace Li, Li Lin, Joshua Liu, Josephine Ly, Janice Ma, Austin Markovic, Hannah Medina, Cristian Mungcal, Jonelle Naranbaatar, Bilguudei Patel, Kayla Petersen, Lauren Phan, Amanda Phung, Malcolm Priasti, Nadiyah Ruano, Nancy Salim, Tanveer Schnell, Kristen Shah, Paras Shen, Jinhua Stutzman, Nathan Sukhina, Alisa Tian, Rayna Vega-Loza, Andrea Wang, Joyce Wang, Jun Watanabe, Rina Wei, Brandon Xie, Lillian Ye, Jessica Zhao, Jeffrey Zimmerman, Jill Bracken, Colton Capili, Jason Char, Andrew Chen, Michel Huang, Pingdi Ji, Sena Kim, Emily Kim, Kenneth Ko, Julie Laput, Sean Louise G Law, Sam Lee, Sang Kuk Lee, Olivia Lim, David Lin, Eric Marik, Kyle Mytych, Josh O'Laughlin, Andie Pak, Jensen Park, Claire Ryu, Ruth Shinde, Ashwin Sosa, Manny Waite, Nick Williams, Mane Wong, Richard Woo, Jocelyn Woo, Jonathan Yepuri, Vishaal Yim, Dorothy Huynh, Dan Wijiewarnasurya, Dinali Shapiro, Casey Levis-Fitzgerald, Marc Jaworski, Leslie Lopatto, David Clark, Ira E Johnson, Tracy Banerjee, Utpal |
author_facet | Evans, Cory J Olson, John M Mondal, Bama Charan Kandimalla, Pratyush Abbasi, Ariano Abdusamad, Mai M Acosta, Osvaldo Ainsworth, Julia A Akram, Haris M Albert, Ralph B Alegria-Leal, Elitzander Alexander, Kai Y Ayala, Angelica C Balashova, Nataliya S Barber, Rebecca M Bassi, Harmanjit Bennion, Sean P Beyder, Miriam Bhatt, Kush V Bhoot, Chinmay Bradshaw, Aaron W Brannigan, Tierney G Cao, Boyu Cashell, Yancey Y Chai, Timothy Chan, Alex W Chan, Carissa Chang, Inho Chang, Jonathan Chang, Michael T Chang, Patrick W Chang, Stephen Chari, Neel Chassiakos, Alexander J Chen, Iris E Chen, Vivian K Chen, Zheying Cheng, Marsha R Chiang, Mimi Chiu, Vivian Choi, Sharon Chung, Jun Ho Contreras, Liset Corona, Edgar Cruz, Courtney J Cruz, Renae L Dang, Jefferson M Dasari, Suhas P De La Fuente, Justin R O Del Rio, Oscar M A Dennis, Emily R Dertsakyan, Petros S Dey, Ipsita Distler, Rachel S Dong, Zhiqiao Dorman, Leah C Douglass, Mark A Ehresman, Allysen B Fu, Ivy H Fua, Andrea Full, Sean M Ghaffari-Rafi, Arash Ghani, Asmar Abdul Giap, Bosco Gill, Sonia Gill, Zafar S Gills, Nicholas J Godavarthi, Sindhuja Golnazarian, Talin Goyal, Raghav Gray, Ricardo Grunfeld, Alexander M Gu, Kelly M Gutierrez, Natalia C Ha, An N Hamid, Iman Hanson, Ashley Hao, Celesti He, Chongbin He, Mengshi Hedtke, Joshua P Hernandez, Ysrael K Hlaing, Hnin Hobby, Faith A Hoi, Karen Hope, Ashley C Hosseinian, Sahra M Hsu, Alice Hsueh, Jennifer Hu, Eileen Hu, Spencer S Huang, Stephanie Huang, Wilson Huynh, Melanie Javier, Carmen Jeon, Na Eun Ji, Sunjong Johal, Jasmin John, Amala Johnson, Lauren Kadakia, Saurin Kakade, Namrata Kamel, Sarah Kaur, Ravinder Khatra, Jagteshwar S Kho, Jeffrey A Kim, Caleb Kim, Emily Jin-Kyung Kim, Hee Jong Kim, Hyun Wook Kim, Jin Hee Kim, Seong Ah Kim, Woo Kyeom Kit, Brian La, Cindy Lai, Jonathan Lam, Vivian Le, Nguyen Khoi Lee, Chi Ju Lee, Dana Lee, Dong Yeon Lee, James Lee, Jason Lee, Jessica Lee, Ju-Yeon Lee, Sharon Lee, Terrence C Lee, Victoria Li, Amber J Li, Jialing Libro, Alexandra M Lien, Irvin C Lim, Mia Lin, Jeffrey M Liu, Connie Y Liu, Steven C Louie, Irene Lu, Shijia W Luo, William Y Luu, Tiffany Madrigal, Josef T Mai, Yishan Miya, Darron I Mohammadi, Mina Mohanta, Sayonika Mokwena, Tebogo Montoya, Tonatiuh Mould, Dallas L Murata, Mark R Muthaiya, Janani Naicker, Seethim Neebe, Mallory R Ngo, Amy Ngo, Duy Q Ngo, Jamie A Nguyen, Anh T Nguyen, Huy C X Nguyen, Rina H Nguyen, Thao T T Nguyen, Vincent T Nishida, Kevin Oh, Seo-Kyung Omi, Kristen M Onglatco, Mary C Almazan, Guadalupe Ortega Paguntalan, Jahzeel Panchal, Maharshi Pang, Stephanie Parikh, Harin B Patel, Purvi D Patel, Trisha H Petersen, Julia E Pham, Steven Phan-Everson, Tien M Pokhriyal, Megha Popovich, Davis W Quaal, Adam T Querubin, Karl Resendiz, Anabel Riabkova, Nadezhda Rong, Fred Salarkia, Sarah Sama, Nateli Sang, Elaine Sanville, David A Schoen, Emily R Shen, Zhouyang Siangchin, Ken Sibal, Gabrielle Sin, Garuem Sjarif, Jasmine Smith, Christopher J Soeboer, Annisa N Sosa, Cristian Spitters, Derek Stender, Bryan Su, Chloe C Summapund, Jenny Sun, Beatrice J Sutanto, Christine Tan, Jaime S Tan, Nguon L Tangmatitam, Parich Trac, Cindy K Tran, Conny Tran, Daniel Tran, Duy Tran, Vina Truong, Patrick A Tsai, Brandon L Tsai, Pei-Hua Tsui, C Kimberly Uriu, Jackson K Venkatesh, Sanan Vo, Maique Vo, Nhat-Thi Vo, Phuong Voros, Timothy C Wan, Yuan Wang, Eric Wang, Jeffrey Wang, Michael K Wang, Yuxuan Wei, Siman Wilson, Matthew N Wong, Daniel Wu, Elliott Xing, Hanning Xu, Jason P Yaftaly, Sahar Yan, Kimberly Yang, Evan Yang, Rebecca Yao, Tony Yeo, Patricia Yip, Vivian Yogi, Puja Young, Gloria Chin Yung, Maggie M Zai, Alexander Zhang, Christine Zhang, Xiao X Zhao, Zijun Zhou, Raymond Zhou, Ziqi Abutouk, Mona Aguirre, Brian Ao, Chon Baranoff, Alexis Beniwal, Angad Cai, Zijie Chan, Ryan Chien, Kenneth Chang Chaudhary, Umar Chin, Patrick Chowdhury, Praptee Dalie, Jamlah Du, Eric Y Estrada, Alec Feng, Erwin Ghaly, Monica Graf, Rose Hernandez, Eduardo Herrera, Kevin Ho, Vivien W Honeychurch, Kaitlyn Hou, Yurianna Huang, Jo M Ishii, Momoko James, Nicholas Jang, Gah-Eun Jin, Daphne Juarez, Jesse Kesaf, Ayse Elif Khalsa, Sat Kartar Kim, Hannah Kovsky, Jenna Kuang, Chak Lon Kumar, Shraddha Lam, Gloria Lee, Ceejay Lee, Grace Li, Li Lin, Joshua Liu, Josephine Ly, Janice Ma, Austin Markovic, Hannah Medina, Cristian Mungcal, Jonelle Naranbaatar, Bilguudei Patel, Kayla Petersen, Lauren Phan, Amanda Phung, Malcolm Priasti, Nadiyah Ruano, Nancy Salim, Tanveer Schnell, Kristen Shah, Paras Shen, Jinhua Stutzman, Nathan Sukhina, Alisa Tian, Rayna Vega-Loza, Andrea Wang, Joyce Wang, Jun Watanabe, Rina Wei, Brandon Xie, Lillian Ye, Jessica Zhao, Jeffrey Zimmerman, Jill Bracken, Colton Capili, Jason Char, Andrew Chen, Michel Huang, Pingdi Ji, Sena Kim, Emily Kim, Kenneth Ko, Julie Laput, Sean Louise G Law, Sam Lee, Sang Kuk Lee, Olivia Lim, David Lin, Eric Marik, Kyle Mytych, Josh O'Laughlin, Andie Pak, Jensen Park, Claire Ryu, Ruth Shinde, Ashwin Sosa, Manny Waite, Nick Williams, Mane Wong, Richard Woo, Jocelyn Woo, Jonathan Yepuri, Vishaal Yim, Dorothy Huynh, Dan Wijiewarnasurya, Dinali Shapiro, Casey Levis-Fitzgerald, Marc Jaworski, Leslie Lopatto, David Clark, Ira E Johnson, Tracy Banerjee, Utpal |
author_sort | Evans, Cory J |
collection | PubMed |
description | Undergraduate students participating in the UCLA Undergraduate Research Consortium for Functional Genomics (URCFG) have conducted a two-phased screen using RNA interference (RNAi) in combination with fluorescent reporter proteins to identify genes important for hematopoiesis in Drosophila. This screen disrupted the function of approximately 3500 genes and identified 137 candidate genes for which loss of function leads to observable changes in the hematopoietic development. Targeting RNAi to maturing, progenitor, and regulatory cell types identified key subsets that either limit or promote blood cell maturation. Bioinformatic analysis reveals gene enrichment in several previously uncharacterized areas, including RNA processing and export and vesicular trafficking. Lastly, the participation of students in this course-based undergraduate research experience (CURE) correlated with increased learning gains across several areas, as well as increased STEM retention, indicating that authentic, student-driven research in the form of a CURE represents an impactful and enriching pedagogical approach. |
format | Online Article Text |
id | pubmed-8022729 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-80227292021-04-09 A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience Evans, Cory J Olson, John M Mondal, Bama Charan Kandimalla, Pratyush Abbasi, Ariano Abdusamad, Mai M Acosta, Osvaldo Ainsworth, Julia A Akram, Haris M Albert, Ralph B Alegria-Leal, Elitzander Alexander, Kai Y Ayala, Angelica C Balashova, Nataliya S Barber, Rebecca M Bassi, Harmanjit Bennion, Sean P Beyder, Miriam Bhatt, Kush V Bhoot, Chinmay Bradshaw, Aaron W Brannigan, Tierney G Cao, Boyu Cashell, Yancey Y Chai, Timothy Chan, Alex W Chan, Carissa Chang, Inho Chang, Jonathan Chang, Michael T Chang, Patrick W Chang, Stephen Chari, Neel Chassiakos, Alexander J Chen, Iris E Chen, Vivian K Chen, Zheying Cheng, Marsha R Chiang, Mimi Chiu, Vivian Choi, Sharon Chung, Jun Ho Contreras, Liset Corona, Edgar Cruz, Courtney J Cruz, Renae L Dang, Jefferson M Dasari, Suhas P De La Fuente, Justin R O Del Rio, Oscar M A Dennis, Emily R Dertsakyan, Petros S Dey, Ipsita Distler, Rachel S Dong, Zhiqiao Dorman, Leah C Douglass, Mark A Ehresman, Allysen B Fu, Ivy H Fua, Andrea Full, Sean M Ghaffari-Rafi, Arash Ghani, Asmar Abdul Giap, Bosco Gill, Sonia Gill, Zafar S Gills, Nicholas J Godavarthi, Sindhuja Golnazarian, Talin Goyal, Raghav Gray, Ricardo Grunfeld, Alexander M Gu, Kelly M Gutierrez, Natalia C Ha, An N Hamid, Iman Hanson, Ashley Hao, Celesti He, Chongbin He, Mengshi Hedtke, Joshua P Hernandez, Ysrael K Hlaing, Hnin Hobby, Faith A Hoi, Karen Hope, Ashley C Hosseinian, Sahra M Hsu, Alice Hsueh, Jennifer Hu, Eileen Hu, Spencer S Huang, Stephanie Huang, Wilson Huynh, Melanie Javier, Carmen Jeon, Na Eun Ji, Sunjong Johal, Jasmin John, Amala Johnson, Lauren Kadakia, Saurin Kakade, Namrata Kamel, Sarah Kaur, Ravinder Khatra, Jagteshwar S Kho, Jeffrey A Kim, Caleb Kim, Emily Jin-Kyung Kim, Hee Jong Kim, Hyun Wook Kim, Jin Hee Kim, Seong Ah Kim, Woo Kyeom Kit, Brian La, Cindy Lai, Jonathan Lam, Vivian Le, Nguyen Khoi Lee, Chi Ju Lee, Dana Lee, Dong Yeon Lee, James Lee, Jason Lee, Jessica Lee, Ju-Yeon Lee, Sharon Lee, Terrence C Lee, Victoria Li, Amber J Li, Jialing Libro, Alexandra M Lien, Irvin C Lim, Mia Lin, Jeffrey M Liu, Connie Y Liu, Steven C Louie, Irene Lu, Shijia W Luo, William Y Luu, Tiffany Madrigal, Josef T Mai, Yishan Miya, Darron I Mohammadi, Mina Mohanta, Sayonika Mokwena, Tebogo Montoya, Tonatiuh Mould, Dallas L Murata, Mark R Muthaiya, Janani Naicker, Seethim Neebe, Mallory R Ngo, Amy Ngo, Duy Q Ngo, Jamie A Nguyen, Anh T Nguyen, Huy C X Nguyen, Rina H Nguyen, Thao T T Nguyen, Vincent T Nishida, Kevin Oh, Seo-Kyung Omi, Kristen M Onglatco, Mary C Almazan, Guadalupe Ortega Paguntalan, Jahzeel Panchal, Maharshi Pang, Stephanie Parikh, Harin B Patel, Purvi D Patel, Trisha H Petersen, Julia E Pham, Steven Phan-Everson, Tien M Pokhriyal, Megha Popovich, Davis W Quaal, Adam T Querubin, Karl Resendiz, Anabel Riabkova, Nadezhda Rong, Fred Salarkia, Sarah Sama, Nateli Sang, Elaine Sanville, David A Schoen, Emily R Shen, Zhouyang Siangchin, Ken Sibal, Gabrielle Sin, Garuem Sjarif, Jasmine Smith, Christopher J Soeboer, Annisa N Sosa, Cristian Spitters, Derek Stender, Bryan Su, Chloe C Summapund, Jenny Sun, Beatrice J Sutanto, Christine Tan, Jaime S Tan, Nguon L Tangmatitam, Parich Trac, Cindy K Tran, Conny Tran, Daniel Tran, Duy Tran, Vina Truong, Patrick A Tsai, Brandon L Tsai, Pei-Hua Tsui, C Kimberly Uriu, Jackson K Venkatesh, Sanan Vo, Maique Vo, Nhat-Thi Vo, Phuong Voros, Timothy C Wan, Yuan Wang, Eric Wang, Jeffrey Wang, Michael K Wang, Yuxuan Wei, Siman Wilson, Matthew N Wong, Daniel Wu, Elliott Xing, Hanning Xu, Jason P Yaftaly, Sahar Yan, Kimberly Yang, Evan Yang, Rebecca Yao, Tony Yeo, Patricia Yip, Vivian Yogi, Puja Young, Gloria Chin Yung, Maggie M Zai, Alexander Zhang, Christine Zhang, Xiao X Zhao, Zijun Zhou, Raymond Zhou, Ziqi Abutouk, Mona Aguirre, Brian Ao, Chon Baranoff, Alexis Beniwal, Angad Cai, Zijie Chan, Ryan Chien, Kenneth Chang Chaudhary, Umar Chin, Patrick Chowdhury, Praptee Dalie, Jamlah Du, Eric Y Estrada, Alec Feng, Erwin Ghaly, Monica Graf, Rose Hernandez, Eduardo Herrera, Kevin Ho, Vivien W Honeychurch, Kaitlyn Hou, Yurianna Huang, Jo M Ishii, Momoko James, Nicholas Jang, Gah-Eun Jin, Daphne Juarez, Jesse Kesaf, Ayse Elif Khalsa, Sat Kartar Kim, Hannah Kovsky, Jenna Kuang, Chak Lon Kumar, Shraddha Lam, Gloria Lee, Ceejay Lee, Grace Li, Li Lin, Joshua Liu, Josephine Ly, Janice Ma, Austin Markovic, Hannah Medina, Cristian Mungcal, Jonelle Naranbaatar, Bilguudei Patel, Kayla Petersen, Lauren Phan, Amanda Phung, Malcolm Priasti, Nadiyah Ruano, Nancy Salim, Tanveer Schnell, Kristen Shah, Paras Shen, Jinhua Stutzman, Nathan Sukhina, Alisa Tian, Rayna Vega-Loza, Andrea Wang, Joyce Wang, Jun Watanabe, Rina Wei, Brandon Xie, Lillian Ye, Jessica Zhao, Jeffrey Zimmerman, Jill Bracken, Colton Capili, Jason Char, Andrew Chen, Michel Huang, Pingdi Ji, Sena Kim, Emily Kim, Kenneth Ko, Julie Laput, Sean Louise G Law, Sam Lee, Sang Kuk Lee, Olivia Lim, David Lin, Eric Marik, Kyle Mytych, Josh O'Laughlin, Andie Pak, Jensen Park, Claire Ryu, Ruth Shinde, Ashwin Sosa, Manny Waite, Nick Williams, Mane Wong, Richard Woo, Jocelyn Woo, Jonathan Yepuri, Vishaal Yim, Dorothy Huynh, Dan Wijiewarnasurya, Dinali Shapiro, Casey Levis-Fitzgerald, Marc Jaworski, Leslie Lopatto, David Clark, Ira E Johnson, Tracy Banerjee, Utpal G3 (Bethesda) Investigation Undergraduate students participating in the UCLA Undergraduate Research Consortium for Functional Genomics (URCFG) have conducted a two-phased screen using RNA interference (RNAi) in combination with fluorescent reporter proteins to identify genes important for hematopoiesis in Drosophila. This screen disrupted the function of approximately 3500 genes and identified 137 candidate genes for which loss of function leads to observable changes in the hematopoietic development. Targeting RNAi to maturing, progenitor, and regulatory cell types identified key subsets that either limit or promote blood cell maturation. Bioinformatic analysis reveals gene enrichment in several previously uncharacterized areas, including RNA processing and export and vesicular trafficking. Lastly, the participation of students in this course-based undergraduate research experience (CURE) correlated with increased learning gains across several areas, as well as increased STEM retention, indicating that authentic, student-driven research in the form of a CURE represents an impactful and enriching pedagogical approach. Oxford University Press 2021-01-30 /pmc/articles/PMC8022729/ /pubmed/33561251 http://dx.doi.org/10.1093/g3journal/jkaa028 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Investigation Evans, Cory J Olson, John M Mondal, Bama Charan Kandimalla, Pratyush Abbasi, Ariano Abdusamad, Mai M Acosta, Osvaldo Ainsworth, Julia A Akram, Haris M Albert, Ralph B Alegria-Leal, Elitzander Alexander, Kai Y Ayala, Angelica C Balashova, Nataliya S Barber, Rebecca M Bassi, Harmanjit Bennion, Sean P Beyder, Miriam Bhatt, Kush V Bhoot, Chinmay Bradshaw, Aaron W Brannigan, Tierney G Cao, Boyu Cashell, Yancey Y Chai, Timothy Chan, Alex W Chan, Carissa Chang, Inho Chang, Jonathan Chang, Michael T Chang, Patrick W Chang, Stephen Chari, Neel Chassiakos, Alexander J Chen, Iris E Chen, Vivian K Chen, Zheying Cheng, Marsha R Chiang, Mimi Chiu, Vivian Choi, Sharon Chung, Jun Ho Contreras, Liset Corona, Edgar Cruz, Courtney J Cruz, Renae L Dang, Jefferson M Dasari, Suhas P De La Fuente, Justin R O Del Rio, Oscar M A Dennis, Emily R Dertsakyan, Petros S Dey, Ipsita Distler, Rachel S Dong, Zhiqiao Dorman, Leah C Douglass, Mark A Ehresman, Allysen B Fu, Ivy H Fua, Andrea Full, Sean M Ghaffari-Rafi, Arash Ghani, Asmar Abdul Giap, Bosco Gill, Sonia Gill, Zafar S Gills, Nicholas J Godavarthi, Sindhuja Golnazarian, Talin Goyal, Raghav Gray, Ricardo Grunfeld, Alexander M Gu, Kelly M Gutierrez, Natalia C Ha, An N Hamid, Iman Hanson, Ashley Hao, Celesti He, Chongbin He, Mengshi Hedtke, Joshua P Hernandez, Ysrael K Hlaing, Hnin Hobby, Faith A Hoi, Karen Hope, Ashley C Hosseinian, Sahra M Hsu, Alice Hsueh, Jennifer Hu, Eileen Hu, Spencer S Huang, Stephanie Huang, Wilson Huynh, Melanie Javier, Carmen Jeon, Na Eun Ji, Sunjong Johal, Jasmin John, Amala Johnson, Lauren Kadakia, Saurin Kakade, Namrata Kamel, Sarah Kaur, Ravinder Khatra, Jagteshwar S Kho, Jeffrey A Kim, Caleb Kim, Emily Jin-Kyung Kim, Hee Jong Kim, Hyun Wook Kim, Jin Hee Kim, Seong Ah Kim, Woo Kyeom Kit, Brian La, Cindy Lai, Jonathan Lam, Vivian Le, Nguyen Khoi Lee, Chi Ju Lee, Dana Lee, Dong Yeon Lee, James Lee, Jason Lee, Jessica Lee, Ju-Yeon Lee, Sharon Lee, Terrence C Lee, Victoria Li, Amber J Li, Jialing Libro, Alexandra M Lien, Irvin C Lim, Mia Lin, Jeffrey M Liu, Connie Y Liu, Steven C Louie, Irene Lu, Shijia W Luo, William Y Luu, Tiffany Madrigal, Josef T Mai, Yishan Miya, Darron I Mohammadi, Mina Mohanta, Sayonika Mokwena, Tebogo Montoya, Tonatiuh Mould, Dallas L Murata, Mark R Muthaiya, Janani Naicker, Seethim Neebe, Mallory R Ngo, Amy Ngo, Duy Q Ngo, Jamie A Nguyen, Anh T Nguyen, Huy C X Nguyen, Rina H Nguyen, Thao T T Nguyen, Vincent T Nishida, Kevin Oh, Seo-Kyung Omi, Kristen M Onglatco, Mary C Almazan, Guadalupe Ortega Paguntalan, Jahzeel Panchal, Maharshi Pang, Stephanie Parikh, Harin B Patel, Purvi D Patel, Trisha H Petersen, Julia E Pham, Steven Phan-Everson, Tien M Pokhriyal, Megha Popovich, Davis W Quaal, Adam T Querubin, Karl Resendiz, Anabel Riabkova, Nadezhda Rong, Fred Salarkia, Sarah Sama, Nateli Sang, Elaine Sanville, David A Schoen, Emily R Shen, Zhouyang Siangchin, Ken Sibal, Gabrielle Sin, Garuem Sjarif, Jasmine Smith, Christopher J Soeboer, Annisa N Sosa, Cristian Spitters, Derek Stender, Bryan Su, Chloe C Summapund, Jenny Sun, Beatrice J Sutanto, Christine Tan, Jaime S Tan, Nguon L Tangmatitam, Parich Trac, Cindy K Tran, Conny Tran, Daniel Tran, Duy Tran, Vina Truong, Patrick A Tsai, Brandon L Tsai, Pei-Hua Tsui, C Kimberly Uriu, Jackson K Venkatesh, Sanan Vo, Maique Vo, Nhat-Thi Vo, Phuong Voros, Timothy C Wan, Yuan Wang, Eric Wang, Jeffrey Wang, Michael K Wang, Yuxuan Wei, Siman Wilson, Matthew N Wong, Daniel Wu, Elliott Xing, Hanning Xu, Jason P Yaftaly, Sahar Yan, Kimberly Yang, Evan Yang, Rebecca Yao, Tony Yeo, Patricia Yip, Vivian Yogi, Puja Young, Gloria Chin Yung, Maggie M Zai, Alexander Zhang, Christine Zhang, Xiao X Zhao, Zijun Zhou, Raymond Zhou, Ziqi Abutouk, Mona Aguirre, Brian Ao, Chon Baranoff, Alexis Beniwal, Angad Cai, Zijie Chan, Ryan Chien, Kenneth Chang Chaudhary, Umar Chin, Patrick Chowdhury, Praptee Dalie, Jamlah Du, Eric Y Estrada, Alec Feng, Erwin Ghaly, Monica Graf, Rose Hernandez, Eduardo Herrera, Kevin Ho, Vivien W Honeychurch, Kaitlyn Hou, Yurianna Huang, Jo M Ishii, Momoko James, Nicholas Jang, Gah-Eun Jin, Daphne Juarez, Jesse Kesaf, Ayse Elif Khalsa, Sat Kartar Kim, Hannah Kovsky, Jenna Kuang, Chak Lon Kumar, Shraddha Lam, Gloria Lee, Ceejay Lee, Grace Li, Li Lin, Joshua Liu, Josephine Ly, Janice Ma, Austin Markovic, Hannah Medina, Cristian Mungcal, Jonelle Naranbaatar, Bilguudei Patel, Kayla Petersen, Lauren Phan, Amanda Phung, Malcolm Priasti, Nadiyah Ruano, Nancy Salim, Tanveer Schnell, Kristen Shah, Paras Shen, Jinhua Stutzman, Nathan Sukhina, Alisa Tian, Rayna Vega-Loza, Andrea Wang, Joyce Wang, Jun Watanabe, Rina Wei, Brandon Xie, Lillian Ye, Jessica Zhao, Jeffrey Zimmerman, Jill Bracken, Colton Capili, Jason Char, Andrew Chen, Michel Huang, Pingdi Ji, Sena Kim, Emily Kim, Kenneth Ko, Julie Laput, Sean Louise G Law, Sam Lee, Sang Kuk Lee, Olivia Lim, David Lin, Eric Marik, Kyle Mytych, Josh O'Laughlin, Andie Pak, Jensen Park, Claire Ryu, Ruth Shinde, Ashwin Sosa, Manny Waite, Nick Williams, Mane Wong, Richard Woo, Jocelyn Woo, Jonathan Yepuri, Vishaal Yim, Dorothy Huynh, Dan Wijiewarnasurya, Dinali Shapiro, Casey Levis-Fitzgerald, Marc Jaworski, Leslie Lopatto, David Clark, Ira E Johnson, Tracy Banerjee, Utpal A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience |
title | A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience |
title_full | A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience |
title_fullStr | A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience |
title_full_unstemmed | A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience |
title_short | A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience |
title_sort | functional genomics screen identifying blood cell development genes in drosophila by undergraduates participating in a course-based research experience |
topic | Investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022729/ https://www.ncbi.nlm.nih.gov/pubmed/33561251 http://dx.doi.org/10.1093/g3journal/jkaa028 |
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