Chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid Angomonas deanei

Angomonas deanei is an endosymbiont-bearing trypanosomatid with several highly fragmented genome assemblies and unknown chromosome number. We present an assembly of the A. deanei nuclear genome based on Oxford Nanopore sequence that resolves into 29 complete or close-to-complete chromosomes. The ass...

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Detalles Bibliográficos
Autores principales: Davey, John W, Catta-Preta, Carolina M C, James, Sally, Forrester, Sarah, Motta, Maria Cristina M, Ashton, Peter D, Mottram, Jeremy C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Genome Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022732/
https://www.ncbi.nlm.nih.gov/pubmed/33561222
http://dx.doi.org/10.1093/g3journal/jkaa018
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author Davey, John W
Catta-Preta, Carolina M C
James, Sally
Forrester, Sarah
Motta, Maria Cristina M
Ashton, Peter D
Mottram, Jeremy C
author_facet Davey, John W
Catta-Preta, Carolina M C
James, Sally
Forrester, Sarah
Motta, Maria Cristina M
Ashton, Peter D
Mottram, Jeremy C
author_sort Davey, John W
collection PubMed
description Angomonas deanei is an endosymbiont-bearing trypanosomatid with several highly fragmented genome assemblies and unknown chromosome number. We present an assembly of the A. deanei nuclear genome based on Oxford Nanopore sequence that resolves into 29 complete or close-to-complete chromosomes. The assembly has several previously unknown special features; it has a supernumerary chromosome, a chromosome with a 340-kb inversion, and there is a translocation between two chromosomes. We also present an updated annotation of the chromosomal genome with 10,365 protein-coding genes, 59 transfer RNAs, 26 ribosomal RNAs, and 62 noncoding RNAs.
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spelling pubmed-80227322021-04-09 Chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid Angomonas deanei Davey, John W Catta-Preta, Carolina M C James, Sally Forrester, Sarah Motta, Maria Cristina M Ashton, Peter D Mottram, Jeremy C G3 (Bethesda) Genome Report Angomonas deanei is an endosymbiont-bearing trypanosomatid with several highly fragmented genome assemblies and unknown chromosome number. We present an assembly of the A. deanei nuclear genome based on Oxford Nanopore sequence that resolves into 29 complete or close-to-complete chromosomes. The assembly has several previously unknown special features; it has a supernumerary chromosome, a chromosome with a 340-kb inversion, and there is a translocation between two chromosomes. We also present an updated annotation of the chromosomal genome with 10,365 protein-coding genes, 59 transfer RNAs, 26 ribosomal RNAs, and 62 noncoding RNAs. Oxford University Press 2020-11-27 /pmc/articles/PMC8022732/ /pubmed/33561222 http://dx.doi.org/10.1093/g3journal/jkaa018 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genome Report
Davey, John W
Catta-Preta, Carolina M C
James, Sally
Forrester, Sarah
Motta, Maria Cristina M
Ashton, Peter D
Mottram, Jeremy C
Chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid Angomonas deanei
title Chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid Angomonas deanei
title_full Chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid Angomonas deanei
title_fullStr Chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid Angomonas deanei
title_full_unstemmed Chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid Angomonas deanei
title_short Chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid Angomonas deanei
title_sort chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid angomonas deanei
topic Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022732/
https://www.ncbi.nlm.nih.gov/pubmed/33561222
http://dx.doi.org/10.1093/g3journal/jkaa018
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