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Color morphs of the coral, Acropora tenuis, show different responses to environmental stress and different expression profiles of fluorescent-protein genes

Corals of the family Acroporidae are key structural components of reefs that support the most diverse marine ecosystems. Due to increasing anthropogenic stresses, coral reefs are in decline. Along the coast of Okinawa, Japan, three different color morphs of Acropora tenuis have been recognized for d...

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Autores principales: Satoh, Noriyuki, Kinjo, Koji, Shintaku, Kohei, Kezuka, Daisuke, Ishimori, Hiroo, Yokokura, Atsushi, Hagiwara, Kazutaka, Hisata, Kanako, Kawamitsu, Mayumi, Koizumi, Koji, Shinzato, Chuya, Zayasu, Yuna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022974/
https://www.ncbi.nlm.nih.gov/pubmed/33621334
http://dx.doi.org/10.1093/g3journal/jkab018
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author Satoh, Noriyuki
Kinjo, Koji
Shintaku, Kohei
Kezuka, Daisuke
Ishimori, Hiroo
Yokokura, Atsushi
Hagiwara, Kazutaka
Hisata, Kanako
Kawamitsu, Mayumi
Koizumi, Koji
Shinzato, Chuya
Zayasu, Yuna
author_facet Satoh, Noriyuki
Kinjo, Koji
Shintaku, Kohei
Kezuka, Daisuke
Ishimori, Hiroo
Yokokura, Atsushi
Hagiwara, Kazutaka
Hisata, Kanako
Kawamitsu, Mayumi
Koizumi, Koji
Shinzato, Chuya
Zayasu, Yuna
author_sort Satoh, Noriyuki
collection PubMed
description Corals of the family Acroporidae are key structural components of reefs that support the most diverse marine ecosystems. Due to increasing anthropogenic stresses, coral reefs are in decline. Along the coast of Okinawa, Japan, three different color morphs of Acropora tenuis have been recognized for decades. These include brown (N morph), yellow green (G), and purple (P) forms. The tips of axial polyps of each morph exhibit specific fluorescence spectra. This attribute is inherited asexually, and color morphs do not change seasonally. In Okinawa Prefecture, during the summer of 2017, N and P morphs experienced bleaching, in which many N morphs died. Dinoflagellates (Symbiodiniaceae) are essential partners of scleractinian corals, and photosynthetic activity of symbionts was reduced in N and P morphs. In contrast, G morphs successfully withstood the stress. Examination of the clade and type of Symbiodiniaceae indicated that the three color-morphs host similar sets of Clade-C symbionts, suggesting that beaching of N and P morphs is unlikely attributable to differences in the clade of Symbiodiniaceae the color morphs hosted. Fluorescent proteins play pivotal roles in physiological regulation of corals. Since the A. tenuis genome has been decoded, we identified five genes for green fluorescent proteins (GFPs), two for cyan fluorescent proteins (CFPs), three for red fluorescent proteins (RFPs), and seven genes for chromoprotein (ChrP). A summer survey of gene expression profiles under outdoor aquarium conditions demonstrated that (a) expression of CFP and REP was quite low during the summer in all three morphs, (b) P morphs expressed higher levels of ChrP than N and G morphs, (c) both N and G morphs expressed GFP more highly than P morphs, and (d) GFP expression in N morphs was reduced during summer whereas G morphs maintained high levels of GFP expression throughout the summer. Although further studies are required to understand the biological significance of these color morphs of A. tenuis, our results suggest that thermal stress resistance is modified by genetic mechanisms that coincidentally lead to diversification of color morphs of this coral.
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spelling pubmed-80229742021-04-09 Color morphs of the coral, Acropora tenuis, show different responses to environmental stress and different expression profiles of fluorescent-protein genes Satoh, Noriyuki Kinjo, Koji Shintaku, Kohei Kezuka, Daisuke Ishimori, Hiroo Yokokura, Atsushi Hagiwara, Kazutaka Hisata, Kanako Kawamitsu, Mayumi Koizumi, Koji Shinzato, Chuya Zayasu, Yuna G3 (Bethesda) Investigation Corals of the family Acroporidae are key structural components of reefs that support the most diverse marine ecosystems. Due to increasing anthropogenic stresses, coral reefs are in decline. Along the coast of Okinawa, Japan, three different color morphs of Acropora tenuis have been recognized for decades. These include brown (N morph), yellow green (G), and purple (P) forms. The tips of axial polyps of each morph exhibit specific fluorescence spectra. This attribute is inherited asexually, and color morphs do not change seasonally. In Okinawa Prefecture, during the summer of 2017, N and P morphs experienced bleaching, in which many N morphs died. Dinoflagellates (Symbiodiniaceae) are essential partners of scleractinian corals, and photosynthetic activity of symbionts was reduced in N and P morphs. In contrast, G morphs successfully withstood the stress. Examination of the clade and type of Symbiodiniaceae indicated that the three color-morphs host similar sets of Clade-C symbionts, suggesting that beaching of N and P morphs is unlikely attributable to differences in the clade of Symbiodiniaceae the color morphs hosted. Fluorescent proteins play pivotal roles in physiological regulation of corals. Since the A. tenuis genome has been decoded, we identified five genes for green fluorescent proteins (GFPs), two for cyan fluorescent proteins (CFPs), three for red fluorescent proteins (RFPs), and seven genes for chromoprotein (ChrP). A summer survey of gene expression profiles under outdoor aquarium conditions demonstrated that (a) expression of CFP and REP was quite low during the summer in all three morphs, (b) P morphs expressed higher levels of ChrP than N and G morphs, (c) both N and G morphs expressed GFP more highly than P morphs, and (d) GFP expression in N morphs was reduced during summer whereas G morphs maintained high levels of GFP expression throughout the summer. Although further studies are required to understand the biological significance of these color morphs of A. tenuis, our results suggest that thermal stress resistance is modified by genetic mechanisms that coincidentally lead to diversification of color morphs of this coral. Oxford University Press 2021-01-23 /pmc/articles/PMC8022974/ /pubmed/33621334 http://dx.doi.org/10.1093/g3journal/jkab018 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Investigation
Satoh, Noriyuki
Kinjo, Koji
Shintaku, Kohei
Kezuka, Daisuke
Ishimori, Hiroo
Yokokura, Atsushi
Hagiwara, Kazutaka
Hisata, Kanako
Kawamitsu, Mayumi
Koizumi, Koji
Shinzato, Chuya
Zayasu, Yuna
Color morphs of the coral, Acropora tenuis, show different responses to environmental stress and different expression profiles of fluorescent-protein genes
title Color morphs of the coral, Acropora tenuis, show different responses to environmental stress and different expression profiles of fluorescent-protein genes
title_full Color morphs of the coral, Acropora tenuis, show different responses to environmental stress and different expression profiles of fluorescent-protein genes
title_fullStr Color morphs of the coral, Acropora tenuis, show different responses to environmental stress and different expression profiles of fluorescent-protein genes
title_full_unstemmed Color morphs of the coral, Acropora tenuis, show different responses to environmental stress and different expression profiles of fluorescent-protein genes
title_short Color morphs of the coral, Acropora tenuis, show different responses to environmental stress and different expression profiles of fluorescent-protein genes
title_sort color morphs of the coral, acropora tenuis, show different responses to environmental stress and different expression profiles of fluorescent-protein genes
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8022974/
https://www.ncbi.nlm.nih.gov/pubmed/33621334
http://dx.doi.org/10.1093/g3journal/jkab018
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