The effect of regular resistance exercise, vitamin D, and calcium supplements on the gastrocnemius muscle in rats in the post-menopausal period: An experimental study

BACKGROUND: Menopause is the natural termination of menstruation which affects the quality and important aspects of women's life. OBJECTIVE: To evaluate the effect of regular resistance training (Ex) with vitamin D (Vit. D) and calcium (Ca) supplements in the postmenopausal period on muscle tissue in rats. MATERIALS AND METHODS: In this experimental study, 72 female Wistar rats (8-10-wk old) were randomly divided into control, placebo, Vit. D, Ca, Ex, Ca + Vit. D, Ex + Ca, Ex + Vit. D, and Ex + Ca + Vit. D groups. Control and placebo groups were fed with a standard diet and sesame oil, respectively. Two month after the ovariectomy, Ex, Ca (35 mg/kg), and Vit. D (10000 IU) were administred in all groups except the control. The number of muscle and inflammatory cells, fiber diameter, endomysium thickness, and degenerative collagen fiber area were assessed through hematoxylin-eosin staining. RESULTS: Muscle cell number was increased in the Ex + Vit. D + Ca, Vit. D + Ex, and Vit. D groups compared to the control group; also, inflammatory cell number showed significant increase in the Ex + Vit. D + Ca (12 [Formula: see text] 5.46), Vit. D + Ex (14 [Formula: see text] 3.25), Ex (13 [Formula: see text] 4.08), Vit. D (11 [Formula: see text] 3.26), Ca + Vit. D (10 [Formula: see text] 1.01), and Ca + Ex (9 [Formula: see text] 2.87) groups. Muscle fiber diameter in the Ex + Vit. D + Ca and Vit. D + Ex groups was higher than the other groups. Endomysium thickness was significantly decreased in the Ex + Vit. D + Ca and Vit. D + Ex groups compared to the control and placebo groups (p [Formula: see text] 0.001). Degenerative collagen fiber area showed a significant increase in the Ex + Vit. D + Ca and Vit. D + Ex groups (p [Formula: see text] 0.001) comparison with the control group. CONCLUSION: Regular resistance exercise, Vit. D, and Ca supplements can improve muscle morphological features in the postmenopausal period.