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The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study
BACKGROUND: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among women. OBJECTIVE: The aim of this study was to investigate the therapeutic effect of hydroalcoholic extract of Nigella sativa (N. sativa) seed as a plant, the consumption of which has been recommended in...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Knowledge E
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023008/ https://www.ncbi.nlm.nih.gov/pubmed/33842824 http://dx.doi.org/10.18502/ijrm.v19i3.8575 |
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author | Khani, Samira Abdollahi, Maasoume Khalaj, Azam Heidari, Hamid Zohali, Somaye |
author_facet | Khani, Samira Abdollahi, Maasoume Khalaj, Azam Heidari, Hamid Zohali, Somaye |
author_sort | Khani, Samira |
collection | PubMed |
description | BACKGROUND: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among women. OBJECTIVE: The aim of this study was to investigate the therapeutic effect of hydroalcoholic extract of Nigella sativa (N. sativa) seed as a plant, the consumption of which has been recommended in Islam, on dehydroepiandrosterone (DHEA)-induced PCOS rats. MATERIALS AND METHODS: This experimental study was carried out on 36 Wistar female rats (3 wk, 60 [Formula: see text] 10 gr). Then rats were divided into 6 groups (n = 6/each): control; PCOS-induced (DHEA 60 mg/kg/sc); PCOS+ Metformine (30 mg/kg); and three experimental groups receiving DHEA + hydroalcoholic extract of N. sativa seeds in doses of 50, 100 and 200 mg/kg, respectively. Blood samples were taken for the evaluation of sexual hormones, oxidative stress, glucose, and insulin after 30 days of treatment. Ovarian tissue was used for histopathological study. RESULTS: The serum levels of luteinizing hormone, testosterone, glucose, insulin resistance, malondialdehyde, and insulin (p [Formula: see text] 0.001) and estrogen increased while the levels of progesterone (p = 0.01) and antioxidant enzymes in the PCOS group decreased (p [Formula: see text] 0.001). CONCLUSION: The administration of the N. sativa extract to the PCOS rats resulted in remarkable changes in the serumic factors relative to the PCOS group. In addition, the extract improved the structure of the ovarian tissue in the PCOS rat. The histopathological results which are in accordance with biochemical findings imply that N. sativa seed could be useful in the treatment of PCOS, the higher doses of the extract being more effective. |
format | Online Article Text |
id | pubmed-8023008 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Knowledge E |
record_format | MEDLINE/PubMed |
spelling | pubmed-80230082021-04-08 The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study Khani, Samira Abdollahi, Maasoume Khalaj, Azam Heidari, Hamid Zohali, Somaye Int J Reprod Biomed Original Article BACKGROUND: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among women. OBJECTIVE: The aim of this study was to investigate the therapeutic effect of hydroalcoholic extract of Nigella sativa (N. sativa) seed as a plant, the consumption of which has been recommended in Islam, on dehydroepiandrosterone (DHEA)-induced PCOS rats. MATERIALS AND METHODS: This experimental study was carried out on 36 Wistar female rats (3 wk, 60 [Formula: see text] 10 gr). Then rats were divided into 6 groups (n = 6/each): control; PCOS-induced (DHEA 60 mg/kg/sc); PCOS+ Metformine (30 mg/kg); and three experimental groups receiving DHEA + hydroalcoholic extract of N. sativa seeds in doses of 50, 100 and 200 mg/kg, respectively. Blood samples were taken for the evaluation of sexual hormones, oxidative stress, glucose, and insulin after 30 days of treatment. Ovarian tissue was used for histopathological study. RESULTS: The serum levels of luteinizing hormone, testosterone, glucose, insulin resistance, malondialdehyde, and insulin (p [Formula: see text] 0.001) and estrogen increased while the levels of progesterone (p = 0.01) and antioxidant enzymes in the PCOS group decreased (p [Formula: see text] 0.001). CONCLUSION: The administration of the N. sativa extract to the PCOS rats resulted in remarkable changes in the serumic factors relative to the PCOS group. In addition, the extract improved the structure of the ovarian tissue in the PCOS rat. The histopathological results which are in accordance with biochemical findings imply that N. sativa seed could be useful in the treatment of PCOS, the higher doses of the extract being more effective. Knowledge E 2021-03-21 /pmc/articles/PMC8023008/ /pubmed/33842824 http://dx.doi.org/10.18502/ijrm.v19i3.8575 Text en Copyright © 2021 Khani et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Original Article Khani, Samira Abdollahi, Maasoume Khalaj, Azam Heidari, Hamid Zohali, Somaye The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study |
title | The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study |
title_full | The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study |
title_fullStr | The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study |
title_full_unstemmed | The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study |
title_short | The effect of hydroalcoholic extract of Nigella Sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: An experimental study |
title_sort | effect of hydroalcoholic extract of nigella sativa seed on dehydroepiandrosterone-induced polycystic ovarian syndrome in rats: an experimental study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023008/ https://www.ncbi.nlm.nih.gov/pubmed/33842824 http://dx.doi.org/10.18502/ijrm.v19i3.8575 |
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