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Virological surveillance of SARS-CoV-2 in an Italian northern area: comparison of Real Time RT PCR cycle threshold (Ct) values in three epidemic periods

Aim of the study was to investigate the differences in Ct values in nasopharingeal swabs collected in three SARS-CoV-2 epidemic periods: first one from February 23 to March 25 (14 days from lockdown started on March 11); the second one from March 26 to May 18 (14 days from the end of strict lockdown...

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Autores principales: Veronesi, Licia, Colucci, Maria Eugenia, Pasquarella, Cesira, Caruso, Luca, Mohieldin Mahgoub Ibrahim, Mostafa, Zoni, Roberta, Pergreffi, Massimiliano, Arcuri, Carlo, Seidenari, Chiara, Viani, Isabella, Capobianco, Emanuela, Mezzetta, Sandra, Affanni, Paola
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mattioli 1885 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023099/
https://www.ncbi.nlm.nih.gov/pubmed/32701912
http://dx.doi.org/10.23750/abm.v91i9-S.10138
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author Veronesi, Licia
Colucci, Maria Eugenia
Pasquarella, Cesira
Caruso, Luca
Mohieldin Mahgoub Ibrahim, Mostafa
Zoni, Roberta
Pergreffi, Massimiliano
Arcuri, Carlo
Seidenari, Chiara
Viani, Isabella
Capobianco, Emanuela
Mezzetta, Sandra
Affanni, Paola
author_facet Veronesi, Licia
Colucci, Maria Eugenia
Pasquarella, Cesira
Caruso, Luca
Mohieldin Mahgoub Ibrahim, Mostafa
Zoni, Roberta
Pergreffi, Massimiliano
Arcuri, Carlo
Seidenari, Chiara
Viani, Isabella
Capobianco, Emanuela
Mezzetta, Sandra
Affanni, Paola
author_sort Veronesi, Licia
collection PubMed
description Aim of the study was to investigate the differences in Ct values in nasopharingeal swabs collected in three SARS-CoV-2 epidemic periods: first one from February 23 to March 25 (14 days from lockdown started on March 11); the second one from March 26 to May 18 (14 days from the end of strict lockdown on May 4) and the third one from May 19 until June 15. Viral RNA was detected in nasopharyngeal swabs obtained both from inpatients and outpatients. COVID-19 infection was confirmed according to the Ct values for N1 and N2 genes ascertained by Real-Time RT-PCR assay as described by the CDC. We calculated the prevalence of nasopharyngeal swabs tested positive for SARS-CoV-2, the mean and median of the Cts and the percentage of samples equal or below the Ct value of 25 in the 3 periods considered. The average value of Ct increased, going from 24.80 in the first epidemic period to 26.64 in the second period to 28.50 in the third period (p <0.001). The percentage of samples with Ct lower than or equal to 25 also decreased sharply from 54.7% to 20.0%. These findings need to be integrated with epidemiological and clinical data. (www.actabiomedica.it)
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spelling pubmed-80230992021-04-07 Virological surveillance of SARS-CoV-2 in an Italian northern area: comparison of Real Time RT PCR cycle threshold (Ct) values in three epidemic periods Veronesi, Licia Colucci, Maria Eugenia Pasquarella, Cesira Caruso, Luca Mohieldin Mahgoub Ibrahim, Mostafa Zoni, Roberta Pergreffi, Massimiliano Arcuri, Carlo Seidenari, Chiara Viani, Isabella Capobianco, Emanuela Mezzetta, Sandra Affanni, Paola Acta Biomed Original Article Aim of the study was to investigate the differences in Ct values in nasopharingeal swabs collected in three SARS-CoV-2 epidemic periods: first one from February 23 to March 25 (14 days from lockdown started on March 11); the second one from March 26 to May 18 (14 days from the end of strict lockdown on May 4) and the third one from May 19 until June 15. Viral RNA was detected in nasopharyngeal swabs obtained both from inpatients and outpatients. COVID-19 infection was confirmed according to the Ct values for N1 and N2 genes ascertained by Real-Time RT-PCR assay as described by the CDC. We calculated the prevalence of nasopharyngeal swabs tested positive for SARS-CoV-2, the mean and median of the Cts and the percentage of samples equal or below the Ct value of 25 in the 3 periods considered. The average value of Ct increased, going from 24.80 in the first epidemic period to 26.64 in the second period to 28.50 in the third period (p <0.001). The percentage of samples with Ct lower than or equal to 25 also decreased sharply from 54.7% to 20.0%. These findings need to be integrated with epidemiological and clinical data. (www.actabiomedica.it) Mattioli 1885 2020 2020-07-20 /pmc/articles/PMC8023099/ /pubmed/32701912 http://dx.doi.org/10.23750/abm.v91i9-S.10138 Text en Copyright: © 2020 ACTA BIO MEDICA SOCIETY OF MEDICINE AND NATURAL SCIENCES OF PARMA http://creativecommons.org/licenses/by-nc-sa/4.0 This work is licensed under a Creative Commons Attribution 4.0 International License
spellingShingle Original Article
Veronesi, Licia
Colucci, Maria Eugenia
Pasquarella, Cesira
Caruso, Luca
Mohieldin Mahgoub Ibrahim, Mostafa
Zoni, Roberta
Pergreffi, Massimiliano
Arcuri, Carlo
Seidenari, Chiara
Viani, Isabella
Capobianco, Emanuela
Mezzetta, Sandra
Affanni, Paola
Virological surveillance of SARS-CoV-2 in an Italian northern area: comparison of Real Time RT PCR cycle threshold (Ct) values in three epidemic periods
title Virological surveillance of SARS-CoV-2 in an Italian northern area: comparison of Real Time RT PCR cycle threshold (Ct) values in three epidemic periods
title_full Virological surveillance of SARS-CoV-2 in an Italian northern area: comparison of Real Time RT PCR cycle threshold (Ct) values in three epidemic periods
title_fullStr Virological surveillance of SARS-CoV-2 in an Italian northern area: comparison of Real Time RT PCR cycle threshold (Ct) values in three epidemic periods
title_full_unstemmed Virological surveillance of SARS-CoV-2 in an Italian northern area: comparison of Real Time RT PCR cycle threshold (Ct) values in three epidemic periods
title_short Virological surveillance of SARS-CoV-2 in an Italian northern area: comparison of Real Time RT PCR cycle threshold (Ct) values in three epidemic periods
title_sort virological surveillance of sars-cov-2 in an italian northern area: comparison of real time rt pcr cycle threshold (ct) values in three epidemic periods
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023099/
https://www.ncbi.nlm.nih.gov/pubmed/32701912
http://dx.doi.org/10.23750/abm.v91i9-S.10138
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