Age-related changes in NG2-expressing telocytes of rat stomach

NG2 immunoreactive cells (NG2 cells) are found in the brain and peripheral tissues including the skin, intestinal tracts, and bladder. In a previous study, we observed the presence of NG2 cells in the stomach using bioluminescence imaging techniques in NG2-firefly luciferase (fLuc) transgenic (Tg) r...

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Autores principales: Tamura, Yasuhisa, Takata, Kumi, Eguchi, Asami, Maeda, Mitsuyo, Kataoka, Yosky
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023479/
https://www.ncbi.nlm.nih.gov/pubmed/33822814
http://dx.doi.org/10.1371/journal.pone.0249729
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author Tamura, Yasuhisa
Takata, Kumi
Eguchi, Asami
Maeda, Mitsuyo
Kataoka, Yosky
author_facet Tamura, Yasuhisa
Takata, Kumi
Eguchi, Asami
Maeda, Mitsuyo
Kataoka, Yosky
author_sort Tamura, Yasuhisa
collection PubMed
description NG2 immunoreactive cells (NG2 cells) are found in the brain and peripheral tissues including the skin, intestinal tracts, and bladder. In a previous study, we observed the presence of NG2 cells in the stomach using bioluminescence imaging techniques in NG2-firefly luciferase (fLuc) transgenic (Tg) rats. Here, we aimed to identify and characterize NG2 cells in the adult rat stomach. Immunohistochemical studies showed that NG2 cells were mainly present in the lamina propria and most of the cells were gastric telocytes, co-expressing CD34, and platelet-derived growth factor receptor alpha (PDGFRα), with a small oval-shaped cell body and extremely long and thin cellular prolongations. In the rat stomach, NG2-expressing telocytes comprised two subpopulations: NG2+/CD34+/PDGFRα+ and NG2+/CD34+/PDGFRα-. Furthermore, we showed that the expression of NG2 gene in the aged rat stomach decreased relative to that of the young rat stomach and the decline of NG2 expression in aged rats was mainly observed in NG2+/CD34+/PDGFRα+ telocytes. These findings suggested age-related alterations in NG2+/CD34+/PDGFRα+ telocytes of rat stomach.
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spelling pubmed-80234792021-04-15 Age-related changes in NG2-expressing telocytes of rat stomach Tamura, Yasuhisa Takata, Kumi Eguchi, Asami Maeda, Mitsuyo Kataoka, Yosky PLoS One Research Article NG2 immunoreactive cells (NG2 cells) are found in the brain and peripheral tissues including the skin, intestinal tracts, and bladder. In a previous study, we observed the presence of NG2 cells in the stomach using bioluminescence imaging techniques in NG2-firefly luciferase (fLuc) transgenic (Tg) rats. Here, we aimed to identify and characterize NG2 cells in the adult rat stomach. Immunohistochemical studies showed that NG2 cells were mainly present in the lamina propria and most of the cells were gastric telocytes, co-expressing CD34, and platelet-derived growth factor receptor alpha (PDGFRα), with a small oval-shaped cell body and extremely long and thin cellular prolongations. In the rat stomach, NG2-expressing telocytes comprised two subpopulations: NG2+/CD34+/PDGFRα+ and NG2+/CD34+/PDGFRα-. Furthermore, we showed that the expression of NG2 gene in the aged rat stomach decreased relative to that of the young rat stomach and the decline of NG2 expression in aged rats was mainly observed in NG2+/CD34+/PDGFRα+ telocytes. These findings suggested age-related alterations in NG2+/CD34+/PDGFRα+ telocytes of rat stomach. Public Library of Science 2021-04-06 /pmc/articles/PMC8023479/ /pubmed/33822814 http://dx.doi.org/10.1371/journal.pone.0249729 Text en © 2021 Tamura et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tamura, Yasuhisa
Takata, Kumi
Eguchi, Asami
Maeda, Mitsuyo
Kataoka, Yosky
Age-related changes in NG2-expressing telocytes of rat stomach
title Age-related changes in NG2-expressing telocytes of rat stomach
title_full Age-related changes in NG2-expressing telocytes of rat stomach
title_fullStr Age-related changes in NG2-expressing telocytes of rat stomach
title_full_unstemmed Age-related changes in NG2-expressing telocytes of rat stomach
title_short Age-related changes in NG2-expressing telocytes of rat stomach
title_sort age-related changes in ng2-expressing telocytes of rat stomach
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023479/
https://www.ncbi.nlm.nih.gov/pubmed/33822814
http://dx.doi.org/10.1371/journal.pone.0249729
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AT maedamitsuyo agerelatedchangesinng2expressingtelocytesofratstomach
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