Cargando…

MicroRNA biomarkers of type 2 diabetes: A protocol for corroborating evidence by computational genomics and meta-analyses

BACKGROUND: Few microRNAs were found consistently dysregulated in type 2 diabetes (T2D) that would gain confidence from Big Pharma to develop diagnostic or therapeutic biomarkers. This study aimed to corroborate evidence from eligible microRNAs-T2D association studies according to stringent quality...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Hongmei, Leung, Siu-wai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023490/
https://www.ncbi.nlm.nih.gov/pubmed/33822793
http://dx.doi.org/10.1371/journal.pone.0247556
_version_ 1783675124618100736
author Zhu, Hongmei
Leung, Siu-wai
author_facet Zhu, Hongmei
Leung, Siu-wai
author_sort Zhu, Hongmei
collection PubMed
description BACKGROUND: Few microRNAs were found consistently dysregulated in type 2 diabetes (T2D) that would gain confidence from Big Pharma to develop diagnostic or therapeutic biomarkers. This study aimed to corroborate evidence from eligible microRNAs-T2D association studies according to stringent quality criteria covering both biological and statistical significance in T2D for biomarker development. METHODS AND ANALYSES: Controlled microRNA expression profiling studies on human with T2D will be retrieved from PubMed, ScienceDirect, and Embase for selecting the statistically significant microRNAs according to pre-specified search strategies and inclusion criteria. Multiple meta-analyses with restricted maximum-likelihood estimation and empirical Bayes estimation under the random-effects model will be conducted by metafor package in R. Subgroup and sensitivity analyses further examine the microRNA candidates for their disease specificity, tissue specificity, blood fraction specificity, and statistical robustness of evidence. Biologically relevant microRNAs will then be selected through genomic database corroboration. Their association with T2D is further measured by area under the curve (AUC) of receive operating characteristic (ROC). Meta-analysis of AUC of potential biomarkers will also be conducted. Enrichment analysis on potential microRNA biomarkers and their target genes will be performed by iPathwayGuide and clusterProfiler, respectively. The corresponding reporting guidelines will be used to assess the quality of included studies according to their profiling methods (microarray, RT-PCR, and RNA-Seq). ETHICS AND DISSEMINATION: No ethics approval is required since this study does not include identifiable personal patient data. PROTOCOL REGISTRATION NUMBER: CRD42017081659.
format Online
Article
Text
id pubmed-8023490
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-80234902021-04-15 MicroRNA biomarkers of type 2 diabetes: A protocol for corroborating evidence by computational genomics and meta-analyses Zhu, Hongmei Leung, Siu-wai PLoS One Registered Report Protocol BACKGROUND: Few microRNAs were found consistently dysregulated in type 2 diabetes (T2D) that would gain confidence from Big Pharma to develop diagnostic or therapeutic biomarkers. This study aimed to corroborate evidence from eligible microRNAs-T2D association studies according to stringent quality criteria covering both biological and statistical significance in T2D for biomarker development. METHODS AND ANALYSES: Controlled microRNA expression profiling studies on human with T2D will be retrieved from PubMed, ScienceDirect, and Embase for selecting the statistically significant microRNAs according to pre-specified search strategies and inclusion criteria. Multiple meta-analyses with restricted maximum-likelihood estimation and empirical Bayes estimation under the random-effects model will be conducted by metafor package in R. Subgroup and sensitivity analyses further examine the microRNA candidates for their disease specificity, tissue specificity, blood fraction specificity, and statistical robustness of evidence. Biologically relevant microRNAs will then be selected through genomic database corroboration. Their association with T2D is further measured by area under the curve (AUC) of receive operating characteristic (ROC). Meta-analysis of AUC of potential biomarkers will also be conducted. Enrichment analysis on potential microRNA biomarkers and their target genes will be performed by iPathwayGuide and clusterProfiler, respectively. The corresponding reporting guidelines will be used to assess the quality of included studies according to their profiling methods (microarray, RT-PCR, and RNA-Seq). ETHICS AND DISSEMINATION: No ethics approval is required since this study does not include identifiable personal patient data. PROTOCOL REGISTRATION NUMBER: CRD42017081659. Public Library of Science 2021-04-06 /pmc/articles/PMC8023490/ /pubmed/33822793 http://dx.doi.org/10.1371/journal.pone.0247556 Text en © 2021 Zhu, Leung http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Registered Report Protocol
Zhu, Hongmei
Leung, Siu-wai
MicroRNA biomarkers of type 2 diabetes: A protocol for corroborating evidence by computational genomics and meta-analyses
title MicroRNA biomarkers of type 2 diabetes: A protocol for corroborating evidence by computational genomics and meta-analyses
title_full MicroRNA biomarkers of type 2 diabetes: A protocol for corroborating evidence by computational genomics and meta-analyses
title_fullStr MicroRNA biomarkers of type 2 diabetes: A protocol for corroborating evidence by computational genomics and meta-analyses
title_full_unstemmed MicroRNA biomarkers of type 2 diabetes: A protocol for corroborating evidence by computational genomics and meta-analyses
title_short MicroRNA biomarkers of type 2 diabetes: A protocol for corroborating evidence by computational genomics and meta-analyses
title_sort microrna biomarkers of type 2 diabetes: a protocol for corroborating evidence by computational genomics and meta-analyses
topic Registered Report Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023490/
https://www.ncbi.nlm.nih.gov/pubmed/33822793
http://dx.doi.org/10.1371/journal.pone.0247556
work_keys_str_mv AT zhuhongmei micrornabiomarkersoftype2diabetesaprotocolforcorroboratingevidencebycomputationalgenomicsandmetaanalyses
AT leungsiuwai micrornabiomarkersoftype2diabetesaprotocolforcorroboratingevidencebycomputationalgenomicsandmetaanalyses