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Rapid Estimation of Binding Constants for Cucurbit[8]uril Ternary Complexes Using Electrochemistry

[Image: see text] Supramolecular complexes are of fundamental interests in biomedicines and adaptive materials, and thus facile methods to determine their binding affinity show usefulness in the design of novel drugs and materials. Herein, we report a novel approach to estimate the binding constants...

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Detalles Bibliográficos
Autores principales: Liu, Jia, Lambert, Hugues, Zhang, Yong-Wei, Lee, Tung-Chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8023530/
https://www.ncbi.nlm.nih.gov/pubmed/33595296
http://dx.doi.org/10.1021/acs.analchem.0c04887
Descripción
Sumario:[Image: see text] Supramolecular complexes are of fundamental interests in biomedicines and adaptive materials, and thus facile methods to determine their binding affinity show usefulness in the design of novel drugs and materials. Herein, we report a novel approach to estimate the binding constants K(G2) of cucurbit[8]uril-methyl viologen-based ternary complexes (CB8-MV(2+)-G2) using electrochemistry, achieving high precision (±0.03) and practical accuracy (±0.32) in logK(G2) and short measurement time (<10 min). In particular, we have uncovered a linear correlation (R(2) > 0.8) between the reduction potential of CB8-MV(2+)-G2 ternary complexes and their reported binding constants from isothermal titration calorimetry, which allow a calibration curve to be plotted based on 25 sample complexes. Mechanistic investigation using experimental and computational approaches reveals that this correlation stems from the dynamic host-guest exchange events occurring after the electron transfer step. Binding constants of unknown ternary complexes, where G2 = hydrocarbons, were estimated, illustrating potential applications for sparsely soluble second guests.