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Post-synthesis nanostructuration of BSA-Capsaicin nanoparticles generated by sucrose excipient

In the pharmaceutical industry nano-hydrocolloid systems frequently coalesce or present nanoparticle aggregation after a long storage periods. Besides, the lyophilization process used to dry nanoparticles (NPs) produces loss of their original properties after dispersion. In this work we evaluated th...

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Autores principales: Carriles, Ramón, Zavala-García, Laura E., Nava-Coronel, Sofía, Sánchez-Arreguín, Alejandro, López, Mercedes G., Sánchez-Segura, Lino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024356/
https://www.ncbi.nlm.nih.gov/pubmed/33824363
http://dx.doi.org/10.1038/s41598-021-87241-8
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author Carriles, Ramón
Zavala-García, Laura E.
Nava-Coronel, Sofía
Sánchez-Arreguín, Alejandro
López, Mercedes G.
Sánchez-Segura, Lino
author_facet Carriles, Ramón
Zavala-García, Laura E.
Nava-Coronel, Sofía
Sánchez-Arreguín, Alejandro
López, Mercedes G.
Sánchez-Segura, Lino
author_sort Carriles, Ramón
collection PubMed
description In the pharmaceutical industry nano-hydrocolloid systems frequently coalesce or present nanoparticle aggregation after a long storage periods. Besides, the lyophilization process used to dry nanoparticles (NPs) produces loss of their original properties after dispersion. In this work we evaluated the effect on morphology and physicochemical properties of different protective excipients during drying of bovine serum albumin (BSA) NPs loaded with different concentrations of capsaicin. Capsaicin concentrations of 0, 812, 1625, 2437, and 3250 µg mL(−1) were used; subsequently, NPs were dried with deionized water (DW), NaCl (DN), sucrose (DS), and not dried (ND). We found that ND, DW, and DN treatments showed a negative effect on the NPs properties; while, DS reduced the aggregation and produced the formation of isolated nanoparticles at higher concentrations of capsaicin (3250 µg mL(−1)), improving their circular shape, morphometrical parameters, and ζ-potential. The stability of the BSA-capsaicin NPs was associated to complex capsaicin/amino acid/water, in which GLY/GLN, ALA/HIS, ARG, THR, TYR, and Iso/CYS amino acids are involved in the restructuration of capsaicin molecules into the surface of nanoparticles during the drying process. The secondary nanostructuration in the post-synthesis stage can improve the molecular stability of the particles and the capacity of entrapping hydrophobic drugs, like capsaicin.
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spelling pubmed-80243562021-04-08 Post-synthesis nanostructuration of BSA-Capsaicin nanoparticles generated by sucrose excipient Carriles, Ramón Zavala-García, Laura E. Nava-Coronel, Sofía Sánchez-Arreguín, Alejandro López, Mercedes G. Sánchez-Segura, Lino Sci Rep Article In the pharmaceutical industry nano-hydrocolloid systems frequently coalesce or present nanoparticle aggregation after a long storage periods. Besides, the lyophilization process used to dry nanoparticles (NPs) produces loss of their original properties after dispersion. In this work we evaluated the effect on morphology and physicochemical properties of different protective excipients during drying of bovine serum albumin (BSA) NPs loaded with different concentrations of capsaicin. Capsaicin concentrations of 0, 812, 1625, 2437, and 3250 µg mL(−1) were used; subsequently, NPs were dried with deionized water (DW), NaCl (DN), sucrose (DS), and not dried (ND). We found that ND, DW, and DN treatments showed a negative effect on the NPs properties; while, DS reduced the aggregation and produced the formation of isolated nanoparticles at higher concentrations of capsaicin (3250 µg mL(−1)), improving their circular shape, morphometrical parameters, and ζ-potential. The stability of the BSA-capsaicin NPs was associated to complex capsaicin/amino acid/water, in which GLY/GLN, ALA/HIS, ARG, THR, TYR, and Iso/CYS amino acids are involved in the restructuration of capsaicin molecules into the surface of nanoparticles during the drying process. The secondary nanostructuration in the post-synthesis stage can improve the molecular stability of the particles and the capacity of entrapping hydrophobic drugs, like capsaicin. Nature Publishing Group UK 2021-04-06 /pmc/articles/PMC8024356/ /pubmed/33824363 http://dx.doi.org/10.1038/s41598-021-87241-8 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Carriles, Ramón
Zavala-García, Laura E.
Nava-Coronel, Sofía
Sánchez-Arreguín, Alejandro
López, Mercedes G.
Sánchez-Segura, Lino
Post-synthesis nanostructuration of BSA-Capsaicin nanoparticles generated by sucrose excipient
title Post-synthesis nanostructuration of BSA-Capsaicin nanoparticles generated by sucrose excipient
title_full Post-synthesis nanostructuration of BSA-Capsaicin nanoparticles generated by sucrose excipient
title_fullStr Post-synthesis nanostructuration of BSA-Capsaicin nanoparticles generated by sucrose excipient
title_full_unstemmed Post-synthesis nanostructuration of BSA-Capsaicin nanoparticles generated by sucrose excipient
title_short Post-synthesis nanostructuration of BSA-Capsaicin nanoparticles generated by sucrose excipient
title_sort post-synthesis nanostructuration of bsa-capsaicin nanoparticles generated by sucrose excipient
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024356/
https://www.ncbi.nlm.nih.gov/pubmed/33824363
http://dx.doi.org/10.1038/s41598-021-87241-8
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