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RNAi efficacy is enhanced by chronic dsRNA feeding in pollen beetle

Double-stranded RNAs (dsRNAs) represent a promising class of biosafe insecticidal compounds. We examined the ability to induce RNA interference (RNAi) in the pollen beetle Brassicogethes aeneus via anther feeding, and compared short-term (3 d) to chronic (17 d) feeding of various concentrations of d...

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Autores principales: Willow, Jonathan, Soonvald, Liina, Sulg, Silva, Kaasik, Riina, Silva, Ana Isabel, Taning, Clauvis Nji Tizi, Christiaens, Olivier, Smagghe, Guy, Veromann, Eve
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024372/
https://www.ncbi.nlm.nih.gov/pubmed/33824392
http://dx.doi.org/10.1038/s42003-021-01975-9
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author Willow, Jonathan
Soonvald, Liina
Sulg, Silva
Kaasik, Riina
Silva, Ana Isabel
Taning, Clauvis Nji Tizi
Christiaens, Olivier
Smagghe, Guy
Veromann, Eve
author_facet Willow, Jonathan
Soonvald, Liina
Sulg, Silva
Kaasik, Riina
Silva, Ana Isabel
Taning, Clauvis Nji Tizi
Christiaens, Olivier
Smagghe, Guy
Veromann, Eve
author_sort Willow, Jonathan
collection PubMed
description Double-stranded RNAs (dsRNAs) represent a promising class of biosafe insecticidal compounds. We examined the ability to induce RNA interference (RNAi) in the pollen beetle Brassicogethes aeneus via anther feeding, and compared short-term (3 d) to chronic (17 d) feeding of various concentrations of dsRNA targeting αCOP (dsαCOP). In short-term dsαCOP feeding, only the highest concentration resulted in significant reductions in B. aeneus survival; whereas in chronic dsαCOP feeding, all three concentrations resulted in significant mortality. Chronic dsαCOP feeding also resulted in significantly greater mortality compared to short-term feeding of equivalent dsαCOP concentrations. Our results have implications for the economics and development of dsRNA spray approaches for managing crop pests, in that multiple lower-concentration dsRNA spray treatments across crop growth stages may result in greater pest management efficacy, compared to single treatments using higher dsRNA concentrations. Furthermore, our results highlight the need for research into the development of RNAi cultivars for oilseed rape protection, given the enhanced RNAi efficacy resulting from chronic, compared to short-term, dsRNA feeding in B. aeneus.
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spelling pubmed-80243722021-04-21 RNAi efficacy is enhanced by chronic dsRNA feeding in pollen beetle Willow, Jonathan Soonvald, Liina Sulg, Silva Kaasik, Riina Silva, Ana Isabel Taning, Clauvis Nji Tizi Christiaens, Olivier Smagghe, Guy Veromann, Eve Commun Biol Article Double-stranded RNAs (dsRNAs) represent a promising class of biosafe insecticidal compounds. We examined the ability to induce RNA interference (RNAi) in the pollen beetle Brassicogethes aeneus via anther feeding, and compared short-term (3 d) to chronic (17 d) feeding of various concentrations of dsRNA targeting αCOP (dsαCOP). In short-term dsαCOP feeding, only the highest concentration resulted in significant reductions in B. aeneus survival; whereas in chronic dsαCOP feeding, all three concentrations resulted in significant mortality. Chronic dsαCOP feeding also resulted in significantly greater mortality compared to short-term feeding of equivalent dsαCOP concentrations. Our results have implications for the economics and development of dsRNA spray approaches for managing crop pests, in that multiple lower-concentration dsRNA spray treatments across crop growth stages may result in greater pest management efficacy, compared to single treatments using higher dsRNA concentrations. Furthermore, our results highlight the need for research into the development of RNAi cultivars for oilseed rape protection, given the enhanced RNAi efficacy resulting from chronic, compared to short-term, dsRNA feeding in B. aeneus. Nature Publishing Group UK 2021-04-06 /pmc/articles/PMC8024372/ /pubmed/33824392 http://dx.doi.org/10.1038/s42003-021-01975-9 Text en © The Author(s) 2021, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Willow, Jonathan
Soonvald, Liina
Sulg, Silva
Kaasik, Riina
Silva, Ana Isabel
Taning, Clauvis Nji Tizi
Christiaens, Olivier
Smagghe, Guy
Veromann, Eve
RNAi efficacy is enhanced by chronic dsRNA feeding in pollen beetle
title RNAi efficacy is enhanced by chronic dsRNA feeding in pollen beetle
title_full RNAi efficacy is enhanced by chronic dsRNA feeding in pollen beetle
title_fullStr RNAi efficacy is enhanced by chronic dsRNA feeding in pollen beetle
title_full_unstemmed RNAi efficacy is enhanced by chronic dsRNA feeding in pollen beetle
title_short RNAi efficacy is enhanced by chronic dsRNA feeding in pollen beetle
title_sort rnai efficacy is enhanced by chronic dsrna feeding in pollen beetle
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024372/
https://www.ncbi.nlm.nih.gov/pubmed/33824392
http://dx.doi.org/10.1038/s42003-021-01975-9
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