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Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing

BACKGROUND: The leather industry generates huge volume of waste each year. Keratin is the principal constituents of this waste that is resistant to degradation. Some bacteria have the ability to degrade keratin through synthesis of a protease called keratinase that can be used as sources of animal f...

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Autores principales: Moonnee, Yeasmin Akter, Foysal, Md Javed, Hashem, Abu, Miah, Md Faruque
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024431/
https://www.ncbi.nlm.nih.gov/pubmed/33825074
http://dx.doi.org/10.1186/s43141-021-00149-8
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author Moonnee, Yeasmin Akter
Foysal, Md Javed
Hashem, Abu
Miah, Md Faruque
author_facet Moonnee, Yeasmin Akter
Foysal, Md Javed
Hashem, Abu
Miah, Md Faruque
author_sort Moonnee, Yeasmin Akter
collection PubMed
description BACKGROUND: The leather industry generates huge volume of waste each year. Keratin is the principal constituents of this waste that is resistant to degradation. Some bacteria have the ability to degrade keratin through synthesis of a protease called keratinase that can be used as sources of animal feed and industrial production of biodiesel, biofertilizer, and bioplastic. Majority of the studies focused on keratin degradation using gram-positive bacteria. Not much of studies are currently available on production of keratinase from gram-negative bacteria and selection of best parameters for the maximum production of enzyme. The aim of this study was to isolate and characterize both groups of bacteria from soil for keratinase and optimize the production parameters. RESULTS: A total of 50 isolates were used for initial screening of enzyme production in skim milk, casein, and feather meal agar. Out of 50, five isolates showed significantly higher enzyme production in preliminary screening assays. Morphological and biochemical characterization revealed 60% of the isolates as gram-negative bacteria including two highest enzyme-producing isolates. The isolates were identified as Pseudomonas aeruginosa through sequencing of 16S rRNA gene. Maximum production of enzyme from P. aeruginosa YK17 was achieved with 2% chicken feather, beef extract, and ammonium nitrate as organic and inorganic nitrogen sources and glucose as a carbon source. Further analysis revealed that 3% inoculum, 40 °C growth temperature and 72-h incubation, resulted in maximum production of keratinase. CONCLUSION: The overall results showed significant higher production of enzyme by the P. aeruginosa YK17 that can be used for the degradation of recalcitrant keratin waste and chemical dehairing in leather industries, thereby preventing environmental pollution. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43141-021-00149-8.
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spelling pubmed-80244312021-04-12 Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing Moonnee, Yeasmin Akter Foysal, Md Javed Hashem, Abu Miah, Md Faruque J Genet Eng Biotechnol Research BACKGROUND: The leather industry generates huge volume of waste each year. Keratin is the principal constituents of this waste that is resistant to degradation. Some bacteria have the ability to degrade keratin through synthesis of a protease called keratinase that can be used as sources of animal feed and industrial production of biodiesel, biofertilizer, and bioplastic. Majority of the studies focused on keratin degradation using gram-positive bacteria. Not much of studies are currently available on production of keratinase from gram-negative bacteria and selection of best parameters for the maximum production of enzyme. The aim of this study was to isolate and characterize both groups of bacteria from soil for keratinase and optimize the production parameters. RESULTS: A total of 50 isolates were used for initial screening of enzyme production in skim milk, casein, and feather meal agar. Out of 50, five isolates showed significantly higher enzyme production in preliminary screening assays. Morphological and biochemical characterization revealed 60% of the isolates as gram-negative bacteria including two highest enzyme-producing isolates. The isolates were identified as Pseudomonas aeruginosa through sequencing of 16S rRNA gene. Maximum production of enzyme from P. aeruginosa YK17 was achieved with 2% chicken feather, beef extract, and ammonium nitrate as organic and inorganic nitrogen sources and glucose as a carbon source. Further analysis revealed that 3% inoculum, 40 °C growth temperature and 72-h incubation, resulted in maximum production of keratinase. CONCLUSION: The overall results showed significant higher production of enzyme by the P. aeruginosa YK17 that can be used for the degradation of recalcitrant keratin waste and chemical dehairing in leather industries, thereby preventing environmental pollution. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s43141-021-00149-8. Springer Berlin Heidelberg 2021-04-06 /pmc/articles/PMC8024431/ /pubmed/33825074 http://dx.doi.org/10.1186/s43141-021-00149-8 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Research
Moonnee, Yeasmin Akter
Foysal, Md Javed
Hashem, Abu
Miah, Md Faruque
Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing
title Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing
title_full Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing
title_fullStr Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing
title_full_unstemmed Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing
title_short Keratinolytic protease from Pseudomonas aeruginosa for leather skin processing
title_sort keratinolytic protease from pseudomonas aeruginosa for leather skin processing
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024431/
https://www.ncbi.nlm.nih.gov/pubmed/33825074
http://dx.doi.org/10.1186/s43141-021-00149-8
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