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Differential Viral Distribution Patterns in Reproductive Tissues of Apis mellifera and Apis cerana Drones

Honeybee drones are male bees that mate with virgin queens during the mating flight, consequently transferring their genes to offspring. Therefore, the health of drones affects the overall fitness of the offspring and ultimately the survivability of the colony. Honeybee viruses are considered to be...

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Autores principales: Phokasem, Patcharin, Liuhao, Wang, Panjad, Poonnawat, Yujie, Tang, Li, Jilian, Chantawannakul, Panuwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024463/
https://www.ncbi.nlm.nih.gov/pubmed/33842568
http://dx.doi.org/10.3389/fvets.2021.608700
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author Phokasem, Patcharin
Liuhao, Wang
Panjad, Poonnawat
Yujie, Tang
Li, Jilian
Chantawannakul, Panuwan
author_facet Phokasem, Patcharin
Liuhao, Wang
Panjad, Poonnawat
Yujie, Tang
Li, Jilian
Chantawannakul, Panuwan
author_sort Phokasem, Patcharin
collection PubMed
description Honeybee drones are male bees that mate with virgin queens during the mating flight, consequently transferring their genes to offspring. Therefore, the health of drones affects the overall fitness of the offspring and ultimately the survivability of the colony. Honeybee viruses are considered to be a major threat to the health of honeybees. In the present study, we demonstrated the pattern of common honeybee viruses in various tissues of drones in the western honeybee, Apis mellifera, and the eastern honeybee, Apis cerana. Drones were collected during the mating flight and analyzed using quantitative real-time (qRT-PCR) to detect the presence of seven honeybee viruses. The qRT-PCR result revealed that three honeybee viruses, namely Black Queen Cell Virus (BQCV), Deformed Wing Virus (DWV), and Chinese Sacbrood Virus (CSBV), were detected in the reproductive tissues of A. mellifera and A. cerana drones. The results from qRT-PCR showed that the Israeli Acute Paralysis Virus (IAPV) was only detected in A. mellifera drone body tissues. Moreover, the prevalence of DWV and BQCV in the drones collected from A. mellifera colonies was significantly higher than that of A. cerana. In addition, virus multiple infections were higher in A. mellifera drones compared to those in A. cerana. CSBV was found predominantly in the reproductive tissues of A. cerana drones. This study is the first report describing the presence of the CSBV in reproductive tissues of A. mellifera drones. Our results may reflect the preference of honeybee viruses in honeybee species and may provide a piece of interesting evidence for understanding the virus transmission in A. cerana.
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spelling pubmed-80244632021-04-08 Differential Viral Distribution Patterns in Reproductive Tissues of Apis mellifera and Apis cerana Drones Phokasem, Patcharin Liuhao, Wang Panjad, Poonnawat Yujie, Tang Li, Jilian Chantawannakul, Panuwan Front Vet Sci Veterinary Science Honeybee drones are male bees that mate with virgin queens during the mating flight, consequently transferring their genes to offspring. Therefore, the health of drones affects the overall fitness of the offspring and ultimately the survivability of the colony. Honeybee viruses are considered to be a major threat to the health of honeybees. In the present study, we demonstrated the pattern of common honeybee viruses in various tissues of drones in the western honeybee, Apis mellifera, and the eastern honeybee, Apis cerana. Drones were collected during the mating flight and analyzed using quantitative real-time (qRT-PCR) to detect the presence of seven honeybee viruses. The qRT-PCR result revealed that three honeybee viruses, namely Black Queen Cell Virus (BQCV), Deformed Wing Virus (DWV), and Chinese Sacbrood Virus (CSBV), were detected in the reproductive tissues of A. mellifera and A. cerana drones. The results from qRT-PCR showed that the Israeli Acute Paralysis Virus (IAPV) was only detected in A. mellifera drone body tissues. Moreover, the prevalence of DWV and BQCV in the drones collected from A. mellifera colonies was significantly higher than that of A. cerana. In addition, virus multiple infections were higher in A. mellifera drones compared to those in A. cerana. CSBV was found predominantly in the reproductive tissues of A. cerana drones. This study is the first report describing the presence of the CSBV in reproductive tissues of A. mellifera drones. Our results may reflect the preference of honeybee viruses in honeybee species and may provide a piece of interesting evidence for understanding the virus transmission in A. cerana. Frontiers Media S.A. 2021-03-24 /pmc/articles/PMC8024463/ /pubmed/33842568 http://dx.doi.org/10.3389/fvets.2021.608700 Text en Copyright © 2021 Phokasem, Liuhao, Panjad, Yujie, Li and Chantawannakul. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Phokasem, Patcharin
Liuhao, Wang
Panjad, Poonnawat
Yujie, Tang
Li, Jilian
Chantawannakul, Panuwan
Differential Viral Distribution Patterns in Reproductive Tissues of Apis mellifera and Apis cerana Drones
title Differential Viral Distribution Patterns in Reproductive Tissues of Apis mellifera and Apis cerana Drones
title_full Differential Viral Distribution Patterns in Reproductive Tissues of Apis mellifera and Apis cerana Drones
title_fullStr Differential Viral Distribution Patterns in Reproductive Tissues of Apis mellifera and Apis cerana Drones
title_full_unstemmed Differential Viral Distribution Patterns in Reproductive Tissues of Apis mellifera and Apis cerana Drones
title_short Differential Viral Distribution Patterns in Reproductive Tissues of Apis mellifera and Apis cerana Drones
title_sort differential viral distribution patterns in reproductive tissues of apis mellifera and apis cerana drones
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024463/
https://www.ncbi.nlm.nih.gov/pubmed/33842568
http://dx.doi.org/10.3389/fvets.2021.608700
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