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Transcriptional Responses of Beauveria bassiana Blastospores Cultured Under Varying Glucose Concentrations

Culturing the entomopathogenic fungus, Beauveria bassiana, under high glucose concentrations coupled with high aeration results in a fungal developmental shift from hyphal growth to mostly blastospores (yeast-like cells). The underlying molecular mechanisms involved in this shift remain elusive. A s...

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Autores principales: Mascarin, Gabriel Moura, Iwanicki, Natasha Sant’Anna, Ramirez, Jose Luis, Delalibera, Ítalo, Dunlap, Christopher A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024584/
https://www.ncbi.nlm.nih.gov/pubmed/33842391
http://dx.doi.org/10.3389/fcimb.2021.644372
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author Mascarin, Gabriel Moura
Iwanicki, Natasha Sant’Anna
Ramirez, Jose Luis
Delalibera, Ítalo
Dunlap, Christopher A.
author_facet Mascarin, Gabriel Moura
Iwanicki, Natasha Sant’Anna
Ramirez, Jose Luis
Delalibera, Ítalo
Dunlap, Christopher A.
author_sort Mascarin, Gabriel Moura
collection PubMed
description Culturing the entomopathogenic fungus, Beauveria bassiana, under high glucose concentrations coupled with high aeration results in a fungal developmental shift from hyphal growth to mostly blastospores (yeast-like cells). The underlying molecular mechanisms involved in this shift remain elusive. A systematic transcriptome analysis of the differential gene expression was preformed to uncover the fungal transcriptomic response to osmotic and oxidative stresses associated with the resulting high blastospore yield. Differential gene expression was compared under moderate (10% w/v) and high (20% w/v) glucose concentrations daily for three days. The RNAseq-based transcriptomic results depicted a higher proportion of downregulated genes when the fungus was grown under 20% glucose than 10%. Additional experiments explored a broader glucose range (4, 8, 12, 16, 20% w/v) with phenotype assessment and qRT-PCR transcript abundance measurements of selected genes. Antioxidant, calcium transport, conidiation, and osmosensor-related genes were highly upregulated in higher glucose titers (16-20%) compared to growth in lower glucose (4-6%) concentrations. The class 1 hydrophobin gene (Hyd1) was highly expressed throughout the culturing. Hyd1 is known to be involved in spore coat rodlet layer assembly, and indicates that blastospores or another cell type containing hydrophobin 1 is expressed in the haemocoel during the infection process. Furthermore, we found implications of the HOG signaling pathway with upregulation of homologous genes Ssk2 and Hog1 for all fermentation time points under hyperosmotic medium (20% glucose). These findings expand our knowledge of the molecular mechanisms behind blastospore development and may help facilitate large-scale industrial production of B. bassiana blastospores for pest control applications.
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spelling pubmed-80245842021-04-08 Transcriptional Responses of Beauveria bassiana Blastospores Cultured Under Varying Glucose Concentrations Mascarin, Gabriel Moura Iwanicki, Natasha Sant’Anna Ramirez, Jose Luis Delalibera, Ítalo Dunlap, Christopher A. Front Cell Infect Microbiol Cellular and Infection Microbiology Culturing the entomopathogenic fungus, Beauveria bassiana, under high glucose concentrations coupled with high aeration results in a fungal developmental shift from hyphal growth to mostly blastospores (yeast-like cells). The underlying molecular mechanisms involved in this shift remain elusive. A systematic transcriptome analysis of the differential gene expression was preformed to uncover the fungal transcriptomic response to osmotic and oxidative stresses associated with the resulting high blastospore yield. Differential gene expression was compared under moderate (10% w/v) and high (20% w/v) glucose concentrations daily for three days. The RNAseq-based transcriptomic results depicted a higher proportion of downregulated genes when the fungus was grown under 20% glucose than 10%. Additional experiments explored a broader glucose range (4, 8, 12, 16, 20% w/v) with phenotype assessment and qRT-PCR transcript abundance measurements of selected genes. Antioxidant, calcium transport, conidiation, and osmosensor-related genes were highly upregulated in higher glucose titers (16-20%) compared to growth in lower glucose (4-6%) concentrations. The class 1 hydrophobin gene (Hyd1) was highly expressed throughout the culturing. Hyd1 is known to be involved in spore coat rodlet layer assembly, and indicates that blastospores or another cell type containing hydrophobin 1 is expressed in the haemocoel during the infection process. Furthermore, we found implications of the HOG signaling pathway with upregulation of homologous genes Ssk2 and Hog1 for all fermentation time points under hyperosmotic medium (20% glucose). These findings expand our knowledge of the molecular mechanisms behind blastospore development and may help facilitate large-scale industrial production of B. bassiana blastospores for pest control applications. Frontiers Media S.A. 2021-03-24 /pmc/articles/PMC8024584/ /pubmed/33842391 http://dx.doi.org/10.3389/fcimb.2021.644372 Text en Copyright © 2021 Mascarin, Iwanicki, Ramirez, Delalibera and Dunlap http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Mascarin, Gabriel Moura
Iwanicki, Natasha Sant’Anna
Ramirez, Jose Luis
Delalibera, Ítalo
Dunlap, Christopher A.
Transcriptional Responses of Beauveria bassiana Blastospores Cultured Under Varying Glucose Concentrations
title Transcriptional Responses of Beauveria bassiana Blastospores Cultured Under Varying Glucose Concentrations
title_full Transcriptional Responses of Beauveria bassiana Blastospores Cultured Under Varying Glucose Concentrations
title_fullStr Transcriptional Responses of Beauveria bassiana Blastospores Cultured Under Varying Glucose Concentrations
title_full_unstemmed Transcriptional Responses of Beauveria bassiana Blastospores Cultured Under Varying Glucose Concentrations
title_short Transcriptional Responses of Beauveria bassiana Blastospores Cultured Under Varying Glucose Concentrations
title_sort transcriptional responses of beauveria bassiana blastospores cultured under varying glucose concentrations
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024584/
https://www.ncbi.nlm.nih.gov/pubmed/33842391
http://dx.doi.org/10.3389/fcimb.2021.644372
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