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Digital scoring of EpCAM and slug expression as prognostic markers in head and neck squamous cell carcinomas

Head and neck squamous cell carcinomas (HNSCCs) have poor clinical outcome owing to therapy resistance and frequent recurrences that are among others attributable to tumor cells in partial epithelial‐to‐mesenchymal transition (pEMT). We compared side‐by‐side software‐based and visual quantification...

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Autores principales: Schinke, Henrik, Heider, Theresa, Herkommer, Timm, Simon, Florian, Blancke Soares, Alexandra, Kranz, Gisela, Samaga, Daniel, Dajka, Laura, Feuchtinger, Annette, Walch, Axel, Valeanu, Laura, Walz, Christoph, Kirchner, Thomas, Canis, Martin, Baumeister, Philipp, Belka, Claus, Maihöfer, Cornelius, Marschner, Sebastian, Pflugradt, Ulrike, Ganswindt, Ute, Hess, Julia, Zitzelsberger, Horst, Gires, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024715/
https://www.ncbi.nlm.nih.gov/pubmed/33340247
http://dx.doi.org/10.1002/1878-0261.12886
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author Schinke, Henrik
Heider, Theresa
Herkommer, Timm
Simon, Florian
Blancke Soares, Alexandra
Kranz, Gisela
Samaga, Daniel
Dajka, Laura
Feuchtinger, Annette
Walch, Axel
Valeanu, Laura
Walz, Christoph
Kirchner, Thomas
Canis, Martin
Baumeister, Philipp
Belka, Claus
Maihöfer, Cornelius
Marschner, Sebastian
Pflugradt, Ulrike
Ganswindt, Ute
Hess, Julia
Zitzelsberger, Horst
Gires, Olivier
author_facet Schinke, Henrik
Heider, Theresa
Herkommer, Timm
Simon, Florian
Blancke Soares, Alexandra
Kranz, Gisela
Samaga, Daniel
Dajka, Laura
Feuchtinger, Annette
Walch, Axel
Valeanu, Laura
Walz, Christoph
Kirchner, Thomas
Canis, Martin
Baumeister, Philipp
Belka, Claus
Maihöfer, Cornelius
Marschner, Sebastian
Pflugradt, Ulrike
Ganswindt, Ute
Hess, Julia
Zitzelsberger, Horst
Gires, Olivier
author_sort Schinke, Henrik
collection PubMed
description Head and neck squamous cell carcinomas (HNSCCs) have poor clinical outcome owing to therapy resistance and frequent recurrences that are among others attributable to tumor cells in partial epithelial‐to‐mesenchymal transition (pEMT). We compared side‐by‐side software‐based and visual quantification of immunohistochemistry (IHC) staining of epithelial marker EpCAM and EMT regulator Slug in n = 102 primary HNSCC to assess optimal analysis protocols. IHC scores incorporated expression levels and percentages of positive cells. Digital and visual evaluation of membrane‐associated EpCAM yielded correlating scorings, whereas visual evaluation of nuclear Slug resulted in significantly higher overall scores. Multivariable Cox proportional hazard analysis defined the median EpCAM expression levels resulting from visual quantification as an independent prognostic factor of overall survival. Slug expression levels resulting from digital quantification were an independent prognostic factor of recurrence‐free survival, locoregional recurrence‐free survival, and disease‐specific survival. Hence, we propose to use visual assessment for the membrane‐associated EpCAM protein, whereas nuclear protein Slug assessment was more accurate following digital measurement.
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spelling pubmed-80247152021-04-12 Digital scoring of EpCAM and slug expression as prognostic markers in head and neck squamous cell carcinomas Schinke, Henrik Heider, Theresa Herkommer, Timm Simon, Florian Blancke Soares, Alexandra Kranz, Gisela Samaga, Daniel Dajka, Laura Feuchtinger, Annette Walch, Axel Valeanu, Laura Walz, Christoph Kirchner, Thomas Canis, Martin Baumeister, Philipp Belka, Claus Maihöfer, Cornelius Marschner, Sebastian Pflugradt, Ulrike Ganswindt, Ute Hess, Julia Zitzelsberger, Horst Gires, Olivier Mol Oncol Research Articles Head and neck squamous cell carcinomas (HNSCCs) have poor clinical outcome owing to therapy resistance and frequent recurrences that are among others attributable to tumor cells in partial epithelial‐to‐mesenchymal transition (pEMT). We compared side‐by‐side software‐based and visual quantification of immunohistochemistry (IHC) staining of epithelial marker EpCAM and EMT regulator Slug in n = 102 primary HNSCC to assess optimal analysis protocols. IHC scores incorporated expression levels and percentages of positive cells. Digital and visual evaluation of membrane‐associated EpCAM yielded correlating scorings, whereas visual evaluation of nuclear Slug resulted in significantly higher overall scores. Multivariable Cox proportional hazard analysis defined the median EpCAM expression levels resulting from visual quantification as an independent prognostic factor of overall survival. Slug expression levels resulting from digital quantification were an independent prognostic factor of recurrence‐free survival, locoregional recurrence‐free survival, and disease‐specific survival. Hence, we propose to use visual assessment for the membrane‐associated EpCAM protein, whereas nuclear protein Slug assessment was more accurate following digital measurement. John Wiley and Sons Inc. 2020-12-29 2021-04 /pmc/articles/PMC8024715/ /pubmed/33340247 http://dx.doi.org/10.1002/1878-0261.12886 Text en © 2020 The Authors. Molecular Oncology published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Schinke, Henrik
Heider, Theresa
Herkommer, Timm
Simon, Florian
Blancke Soares, Alexandra
Kranz, Gisela
Samaga, Daniel
Dajka, Laura
Feuchtinger, Annette
Walch, Axel
Valeanu, Laura
Walz, Christoph
Kirchner, Thomas
Canis, Martin
Baumeister, Philipp
Belka, Claus
Maihöfer, Cornelius
Marschner, Sebastian
Pflugradt, Ulrike
Ganswindt, Ute
Hess, Julia
Zitzelsberger, Horst
Gires, Olivier
Digital scoring of EpCAM and slug expression as prognostic markers in head and neck squamous cell carcinomas
title Digital scoring of EpCAM and slug expression as prognostic markers in head and neck squamous cell carcinomas
title_full Digital scoring of EpCAM and slug expression as prognostic markers in head and neck squamous cell carcinomas
title_fullStr Digital scoring of EpCAM and slug expression as prognostic markers in head and neck squamous cell carcinomas
title_full_unstemmed Digital scoring of EpCAM and slug expression as prognostic markers in head and neck squamous cell carcinomas
title_short Digital scoring of EpCAM and slug expression as prognostic markers in head and neck squamous cell carcinomas
title_sort digital scoring of epcam and slug expression as prognostic markers in head and neck squamous cell carcinomas
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024715/
https://www.ncbi.nlm.nih.gov/pubmed/33340247
http://dx.doi.org/10.1002/1878-0261.12886
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