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Tetraspanin 1 promotes endometriosis leading to ovarian clear cell carcinoma
Ovarian clear cell carcinoma (OCCC) reportedly develops from endometriosis. However, the molecular mechanism underlying its malignant progression to OCCC remains elusive. This study aimed to identify an essential gene in the malignant transformation of endometriosis to OCCC. We performed RNA sequenc...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024726/ https://www.ncbi.nlm.nih.gov/pubmed/33331115 http://dx.doi.org/10.1002/1878-0261.12884 |
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author | Shin, Ha‐Yeon Yang, Wookyeom Chay, Doo Byung Lee, Eun‐ju Chung, Joon‐Yong Kim, Hyun‐Soo Kim, Jae‐Hoon |
author_facet | Shin, Ha‐Yeon Yang, Wookyeom Chay, Doo Byung Lee, Eun‐ju Chung, Joon‐Yong Kim, Hyun‐Soo Kim, Jae‐Hoon |
author_sort | Shin, Ha‐Yeon |
collection | PubMed |
description | Ovarian clear cell carcinoma (OCCC) reportedly develops from endometriosis. However, the molecular mechanism underlying its malignant progression to OCCC remains elusive. This study aimed to identify an essential gene in the malignant transformation of endometriosis to OCCC. We performed RNA sequencing in formalin‐fixed, paraffin‐embedded (FFPE) tissues of endometriosis (n = 9), atypical endometriosis (AtyEm) (n = 18), adjacent endometriosis to OCCC (AdjEm) (n = 7), and OCCC (n = 17). We found that tetraspanin 1 (TSPAN1) mRNA level was significantly increased by 2.4‐ (DESeq2) and 3.4‐fold (edgeR) in AtyEm and by 80.7‐ (DESeq2) and 101‐fold (edgeR) in OCCC relative to endometriosis. We confirmed that TSPAN1 protein level was similarly overexpressed in OCCC tissues and cell lines. In immortalized endometriosis cell lines, TSPAN1 overexpression enhanced cell growth and invasion. Mechanistically, TSPAN1 triggered AMP‐activated protein kinase (AMPK) activity, promoting endometriosis and cell growth. Upregulated levels of TSPAN1 are considered an early event in the development of high‐risk endometriosis that could progress to ovarian cancer. Our study suggests the potential of TSPAN1 as a screening candidate for high‐risk endometriosis. |
format | Online Article Text |
id | pubmed-8024726 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80247262021-04-12 Tetraspanin 1 promotes endometriosis leading to ovarian clear cell carcinoma Shin, Ha‐Yeon Yang, Wookyeom Chay, Doo Byung Lee, Eun‐ju Chung, Joon‐Yong Kim, Hyun‐Soo Kim, Jae‐Hoon Mol Oncol Research Articles Ovarian clear cell carcinoma (OCCC) reportedly develops from endometriosis. However, the molecular mechanism underlying its malignant progression to OCCC remains elusive. This study aimed to identify an essential gene in the malignant transformation of endometriosis to OCCC. We performed RNA sequencing in formalin‐fixed, paraffin‐embedded (FFPE) tissues of endometriosis (n = 9), atypical endometriosis (AtyEm) (n = 18), adjacent endometriosis to OCCC (AdjEm) (n = 7), and OCCC (n = 17). We found that tetraspanin 1 (TSPAN1) mRNA level was significantly increased by 2.4‐ (DESeq2) and 3.4‐fold (edgeR) in AtyEm and by 80.7‐ (DESeq2) and 101‐fold (edgeR) in OCCC relative to endometriosis. We confirmed that TSPAN1 protein level was similarly overexpressed in OCCC tissues and cell lines. In immortalized endometriosis cell lines, TSPAN1 overexpression enhanced cell growth and invasion. Mechanistically, TSPAN1 triggered AMP‐activated protein kinase (AMPK) activity, promoting endometriosis and cell growth. Upregulated levels of TSPAN1 are considered an early event in the development of high‐risk endometriosis that could progress to ovarian cancer. Our study suggests the potential of TSPAN1 as a screening candidate for high‐risk endometriosis. John Wiley and Sons Inc. 2021-01-07 2021-04 /pmc/articles/PMC8024726/ /pubmed/33331115 http://dx.doi.org/10.1002/1878-0261.12884 Text en © 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Shin, Ha‐Yeon Yang, Wookyeom Chay, Doo Byung Lee, Eun‐ju Chung, Joon‐Yong Kim, Hyun‐Soo Kim, Jae‐Hoon Tetraspanin 1 promotes endometriosis leading to ovarian clear cell carcinoma |
title | Tetraspanin 1 promotes endometriosis leading to ovarian clear cell carcinoma |
title_full | Tetraspanin 1 promotes endometriosis leading to ovarian clear cell carcinoma |
title_fullStr | Tetraspanin 1 promotes endometriosis leading to ovarian clear cell carcinoma |
title_full_unstemmed | Tetraspanin 1 promotes endometriosis leading to ovarian clear cell carcinoma |
title_short | Tetraspanin 1 promotes endometriosis leading to ovarian clear cell carcinoma |
title_sort | tetraspanin 1 promotes endometriosis leading to ovarian clear cell carcinoma |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024726/ https://www.ncbi.nlm.nih.gov/pubmed/33331115 http://dx.doi.org/10.1002/1878-0261.12884 |
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