Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons

Calcium regulation is a critical process in neurons, and Ca(2+) signaling is a major contributor to neurological disorders including Parkinson’s disease (PD). Here, combining calcium imaging with whole-cell Ca(2+) current recording, we provide a detailed protocol for measuring Ca(2+) homeostasis in...

Descripción completa

Detalles Bibliográficos
Autores principales: Yin, Xiling, Kim, Jungwoo Wren, Liu, Shiyu, Dawson, Ted M., Dawson, Valina L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024766/
https://www.ncbi.nlm.nih.gov/pubmed/33855307
http://dx.doi.org/10.1016/j.xpro.2021.100405
Descripción
Sumario:Calcium regulation is a critical process in neurons, and Ca(2+) signaling is a major contributor to neurological disorders including Parkinson’s disease (PD). Here, combining calcium imaging with whole-cell Ca(2+) current recording, we provide a detailed protocol for measuring Ca(2+) homeostasis in dopaminergic (DA) neurons derived from human induced pluripotent stem cells (hiPSCs). This approach can be applied to investigate the role of Ca(2+) homeostasis in neuronal functionality as well as in disease processes. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020).

Ejemplares similares