Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons

Calcium regulation is a critical process in neurons, and Ca(2+) signaling is a major contributor to neurological disorders including Parkinson’s disease (PD). Here, combining calcium imaging with whole-cell Ca(2+) current recording, we provide a detailed protocol for measuring Ca(2+) homeostasis in...

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Detalles Bibliográficos
Autores principales: Yin, Xiling, Kim, Jungwoo Wren, Liu, Shiyu, Dawson, Ted M., Dawson, Valina L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024766/
https://www.ncbi.nlm.nih.gov/pubmed/33855307
http://dx.doi.org/10.1016/j.xpro.2021.100405
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author Yin, Xiling
Kim, Jungwoo Wren
Liu, Shiyu
Dawson, Ted M.
Dawson, Valina L.
author_facet Yin, Xiling
Kim, Jungwoo Wren
Liu, Shiyu
Dawson, Ted M.
Dawson, Valina L.
author_sort Yin, Xiling
collection PubMed
description Calcium regulation is a critical process in neurons, and Ca(2+) signaling is a major contributor to neurological disorders including Parkinson’s disease (PD). Here, combining calcium imaging with whole-cell Ca(2+) current recording, we provide a detailed protocol for measuring Ca(2+) homeostasis in dopaminergic (DA) neurons derived from human induced pluripotent stem cells (hiPSCs). This approach can be applied to investigate the role of Ca(2+) homeostasis in neuronal functionality as well as in disease processes. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020).
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spelling pubmed-80247662021-04-13 Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons Yin, Xiling Kim, Jungwoo Wren Liu, Shiyu Dawson, Ted M. Dawson, Valina L. STAR Protoc Protocol Calcium regulation is a critical process in neurons, and Ca(2+) signaling is a major contributor to neurological disorders including Parkinson’s disease (PD). Here, combining calcium imaging with whole-cell Ca(2+) current recording, we provide a detailed protocol for measuring Ca(2+) homeostasis in dopaminergic (DA) neurons derived from human induced pluripotent stem cells (hiPSCs). This approach can be applied to investigate the role of Ca(2+) homeostasis in neuronal functionality as well as in disease processes. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020). Elsevier 2021-03-23 /pmc/articles/PMC8024766/ /pubmed/33855307 http://dx.doi.org/10.1016/j.xpro.2021.100405 Text en © 2021 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Yin, Xiling
Kim, Jungwoo Wren
Liu, Shiyu
Dawson, Ted M.
Dawson, Valina L.
Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons
title Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons
title_full Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons
title_fullStr Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons
title_full_unstemmed Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons
title_short Protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons
title_sort protocol for measurement of calcium dysregulation in human induced pluripotent stem cell-derived dopaminergic neurons
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024766/
https://www.ncbi.nlm.nih.gov/pubmed/33855307
http://dx.doi.org/10.1016/j.xpro.2021.100405
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