Protocol for fractionation-assisted native ChIP (fanChIP) to capture protein-protein/DNA interactions on chromatin

Conventional chromatin immunoprecipitation (ChIP) includes many steps that need to be optimized. Here, we have described a protocol of fractionation-assisted native ChIP (fanChIP) which combines subfractionation and native ChIP to purify protein/chromatin complexes applicable for analyses of both pr...

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Detalles Bibliográficos
Autores principales: Miyamoto, Ryo, Yokoyama, Akihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024768/
https://www.ncbi.nlm.nih.gov/pubmed/33855306
http://dx.doi.org/10.1016/j.xpro.2021.100404

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024768/
https://www.ncbi.nlm.nih.gov/pubmed/33855306
http://dx.doi.org/10.1016/j.xpro.2021.100404

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