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Identification of differentially expressed genes and the role of PDK4 in CD14(+) monocytes of coronary artery disease

Background. Coronary artery disease (CAD) is a chronic inflammatory disease caused by development of atherosclerosis (AS), which is the leading cause of mortality and disability. Our study aimed to identify the differentially expressed genes (DEGs) in CD14(+) monocytes from CAD patients compared wit...

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Autores principales: Du, Pei, Guo, Ren, Gao, Keqin, Yang, Shuang, Yao, Baige, Cui, Haobo, Zhao, Ming, Jia, Sujie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024870/
https://www.ncbi.nlm.nih.gov/pubmed/33739370
http://dx.doi.org/10.1042/BSR20204124
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author Du, Pei
Guo, Ren
Gao, Keqin
Yang, Shuang
Yao, Baige
Cui, Haobo
Zhao, Ming
Jia, Sujie
author_facet Du, Pei
Guo, Ren
Gao, Keqin
Yang, Shuang
Yao, Baige
Cui, Haobo
Zhao, Ming
Jia, Sujie
author_sort Du, Pei
collection PubMed
description Background. Coronary artery disease (CAD) is a chronic inflammatory disease caused by development of atherosclerosis (AS), which is the leading cause of mortality and disability. Our study aimed to identify the differentially expressed genes (DEGs) in CD14(+) monocytes from CAD patients compared with those from non-CAD controls, which might pave the way to diagnosis and treatment for CAD. Methods. The RNA-sequencing (RNA-seq) was performed by BGISEQ-500, followed by analyzing with R package to screening DEGs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed by R package. In addition, we validated the results of RNA-seq using real-time quantitative polymerase chain reaction (RT-qPCR). Furthermore, we explored the function of selected ten genes in LDL-treated CD14(+) monocytes by RT-qPCR. Results. a total of 2897 DEGs were identified, including 753 up- and 2144 down-regulated genes in CD14(+) monocytes from CAD patients. These DEGs were mainly enriched in plasma membrane and cell periphery of cell component, immune system process of biological process, NF-κB signaling pathway, cell adhesion molecules signaling pathway and cytokine–cytokine receptor interaction signaling pathway. In LDL-treated CD14(+) monocytes, the mRNA expression of pyruvate dehydrogenase kinase 4 (PDK4) was significantly up-regulated. Conclusion. In the present study, we suggested that PDK4 might play a role in progression of CAD. The study will provide some pieces of evidence to investigate the role and mechanism of key genes in the pathogenesis of CAD.
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spelling pubmed-80248702021-04-15 Identification of differentially expressed genes and the role of PDK4 in CD14(+) monocytes of coronary artery disease Du, Pei Guo, Ren Gao, Keqin Yang, Shuang Yao, Baige Cui, Haobo Zhao, Ming Jia, Sujie Biosci Rep Cardiovascular System & Vascular Biology Background. Coronary artery disease (CAD) is a chronic inflammatory disease caused by development of atherosclerosis (AS), which is the leading cause of mortality and disability. Our study aimed to identify the differentially expressed genes (DEGs) in CD14(+) monocytes from CAD patients compared with those from non-CAD controls, which might pave the way to diagnosis and treatment for CAD. Methods. The RNA-sequencing (RNA-seq) was performed by BGISEQ-500, followed by analyzing with R package to screening DEGs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed by R package. In addition, we validated the results of RNA-seq using real-time quantitative polymerase chain reaction (RT-qPCR). Furthermore, we explored the function of selected ten genes in LDL-treated CD14(+) monocytes by RT-qPCR. Results. a total of 2897 DEGs were identified, including 753 up- and 2144 down-regulated genes in CD14(+) monocytes from CAD patients. These DEGs were mainly enriched in plasma membrane and cell periphery of cell component, immune system process of biological process, NF-κB signaling pathway, cell adhesion molecules signaling pathway and cytokine–cytokine receptor interaction signaling pathway. In LDL-treated CD14(+) monocytes, the mRNA expression of pyruvate dehydrogenase kinase 4 (PDK4) was significantly up-regulated. Conclusion. In the present study, we suggested that PDK4 might play a role in progression of CAD. The study will provide some pieces of evidence to investigate the role and mechanism of key genes in the pathogenesis of CAD. Portland Press Ltd. 2021-04-06 /pmc/articles/PMC8024870/ /pubmed/33739370 http://dx.doi.org/10.1042/BSR20204124 Text en © 2021 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Cardiovascular System & Vascular Biology
Du, Pei
Guo, Ren
Gao, Keqin
Yang, Shuang
Yao, Baige
Cui, Haobo
Zhao, Ming
Jia, Sujie
Identification of differentially expressed genes and the role of PDK4 in CD14(+) monocytes of coronary artery disease
title Identification of differentially expressed genes and the role of PDK4 in CD14(+) monocytes of coronary artery disease
title_full Identification of differentially expressed genes and the role of PDK4 in CD14(+) monocytes of coronary artery disease
title_fullStr Identification of differentially expressed genes and the role of PDK4 in CD14(+) monocytes of coronary artery disease
title_full_unstemmed Identification of differentially expressed genes and the role of PDK4 in CD14(+) monocytes of coronary artery disease
title_short Identification of differentially expressed genes and the role of PDK4 in CD14(+) monocytes of coronary artery disease
title_sort identification of differentially expressed genes and the role of pdk4 in cd14(+) monocytes of coronary artery disease
topic Cardiovascular System & Vascular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8024870/
https://www.ncbi.nlm.nih.gov/pubmed/33739370
http://dx.doi.org/10.1042/BSR20204124
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