Cargando…
Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model
BACKGROUND: To describe the effect of mechanical ventilation on diaphragm mitochondrial oxygen consumption, ATP production, reactive oxygen species (ROS) generation, and cytochrome c oxidase activity and content, and their relationship to diaphragm strength in an experimental model of sepsis. METHOD...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025065/ https://www.ncbi.nlm.nih.gov/pubmed/33825987 http://dx.doi.org/10.1186/s40635-021-00384-w |
_version_ | 1783675438128693248 |
---|---|
author | Eyenga, P. Roussel, D. Rey, B. Ndille, P. Teulier, L. Eyenga, F. Romestaing, C. Morel, J. Gueguen-Chaignon, V. Sheu, S-S. |
author_facet | Eyenga, P. Roussel, D. Rey, B. Ndille, P. Teulier, L. Eyenga, F. Romestaing, C. Morel, J. Gueguen-Chaignon, V. Sheu, S-S. |
author_sort | Eyenga, P. |
collection | PubMed |
description | BACKGROUND: To describe the effect of mechanical ventilation on diaphragm mitochondrial oxygen consumption, ATP production, reactive oxygen species (ROS) generation, and cytochrome c oxidase activity and content, and their relationship to diaphragm strength in an experimental model of sepsis. METHODS: A cecal ligation and puncture (CLP) protocol was performed in 12 rats while 12 controls underwent sham operation. Half of the rats in each group were paralyzed and mechanically ventilated. We performed blood gas analysis and lactic acid assays 6 h after surgery. Afterwards, we measured diaphragm strength and mitochondrial oxygen consumption, ATP and ROS generation, and cytochrome c oxidase activity. We also measured malondialdehyde (MDA) content as an index of lipid peroxidation, and mRNA expression of the proinflammatory interleukin-1β (IL-1β) in diaphragms. RESULTS: CLP rats showed severe hypotension, metabolic acidosis, and upregulation of diaphragm IL-1β mRNA expression. Compared to sham controls, spontaneously breathing CLP rats showed lower diaphragm force and increased susceptibility to fatigue, along with depressed mitochondrial oxygen consumption and ATP production and cytochrome c oxidase activity. These rats also showed increased mitochondrial ROS generation and MDA content. Mechanical ventilation markedly restored mitochondrial oxygen consumption and ATP production in CLP rats; lowered mitochondrial ROS production by the complex 3; and preserved cytochrome c oxidase activity. CONCLUSION: In an experimental model of sepsis, early initiation of mechanical ventilation restores diaphragm mitochondrial function. |
format | Online Article Text |
id | pubmed-8025065 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-80250652021-04-07 Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model Eyenga, P. Roussel, D. Rey, B. Ndille, P. Teulier, L. Eyenga, F. Romestaing, C. Morel, J. Gueguen-Chaignon, V. Sheu, S-S. Intensive Care Med Exp Research Articles BACKGROUND: To describe the effect of mechanical ventilation on diaphragm mitochondrial oxygen consumption, ATP production, reactive oxygen species (ROS) generation, and cytochrome c oxidase activity and content, and their relationship to diaphragm strength in an experimental model of sepsis. METHODS: A cecal ligation and puncture (CLP) protocol was performed in 12 rats while 12 controls underwent sham operation. Half of the rats in each group were paralyzed and mechanically ventilated. We performed blood gas analysis and lactic acid assays 6 h after surgery. Afterwards, we measured diaphragm strength and mitochondrial oxygen consumption, ATP and ROS generation, and cytochrome c oxidase activity. We also measured malondialdehyde (MDA) content as an index of lipid peroxidation, and mRNA expression of the proinflammatory interleukin-1β (IL-1β) in diaphragms. RESULTS: CLP rats showed severe hypotension, metabolic acidosis, and upregulation of diaphragm IL-1β mRNA expression. Compared to sham controls, spontaneously breathing CLP rats showed lower diaphragm force and increased susceptibility to fatigue, along with depressed mitochondrial oxygen consumption and ATP production and cytochrome c oxidase activity. These rats also showed increased mitochondrial ROS generation and MDA content. Mechanical ventilation markedly restored mitochondrial oxygen consumption and ATP production in CLP rats; lowered mitochondrial ROS production by the complex 3; and preserved cytochrome c oxidase activity. CONCLUSION: In an experimental model of sepsis, early initiation of mechanical ventilation restores diaphragm mitochondrial function. Springer International Publishing 2021-04-07 /pmc/articles/PMC8025065/ /pubmed/33825987 http://dx.doi.org/10.1186/s40635-021-00384-w Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Articles Eyenga, P. Roussel, D. Rey, B. Ndille, P. Teulier, L. Eyenga, F. Romestaing, C. Morel, J. Gueguen-Chaignon, V. Sheu, S-S. Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model |
title | Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model |
title_full | Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model |
title_fullStr | Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model |
title_full_unstemmed | Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model |
title_short | Mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model |
title_sort | mechanical ventilation preserves diaphragm mitochondrial function in a rat sepsis model |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025065/ https://www.ncbi.nlm.nih.gov/pubmed/33825987 http://dx.doi.org/10.1186/s40635-021-00384-w |
work_keys_str_mv | AT eyengap mechanicalventilationpreservesdiaphragmmitochondrialfunctioninaratsepsismodel AT rousseld mechanicalventilationpreservesdiaphragmmitochondrialfunctioninaratsepsismodel AT reyb mechanicalventilationpreservesdiaphragmmitochondrialfunctioninaratsepsismodel AT ndillep mechanicalventilationpreservesdiaphragmmitochondrialfunctioninaratsepsismodel AT teulierl mechanicalventilationpreservesdiaphragmmitochondrialfunctioninaratsepsismodel AT eyengaf mechanicalventilationpreservesdiaphragmmitochondrialfunctioninaratsepsismodel AT romestaingc mechanicalventilationpreservesdiaphragmmitochondrialfunctioninaratsepsismodel AT morelj mechanicalventilationpreservesdiaphragmmitochondrialfunctioninaratsepsismodel AT gueguenchaignonv mechanicalventilationpreservesdiaphragmmitochondrialfunctioninaratsepsismodel AT sheuss mechanicalventilationpreservesdiaphragmmitochondrialfunctioninaratsepsismodel |