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USP18-deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal-dependent manner
Ubiquitin-specific peptidase (USP)18 belongs to the USP family, and is involved in cleaving and removing ubiquitin or ubiquitin-like molecules from their target molecules. Recently, increasing evidence has suggested that USP18 is constitutively expressed in different types of human tumors, and ectop...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025074/ https://www.ncbi.nlm.nih.gov/pubmed/33850562 http://dx.doi.org/10.3892/ol.2021.12682 |
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author | Pan, Aonan Li, Yue Guan, Jian Zhang, Pengxia Zhang, Chunbin Han, Yupeng Zhang, Tao Cheng, Yao Sun, Luo Lu, Shizhen Weng, Jinru Ren, Qiaosheng Fan, Shengjie Wang, Weiqun Wang, Jingtao |
author_facet | Pan, Aonan Li, Yue Guan, Jian Zhang, Pengxia Zhang, Chunbin Han, Yupeng Zhang, Tao Cheng, Yao Sun, Luo Lu, Shizhen Weng, Jinru Ren, Qiaosheng Fan, Shengjie Wang, Weiqun Wang, Jingtao |
author_sort | Pan, Aonan |
collection | PubMed |
description | Ubiquitin-specific peptidase (USP)18 belongs to the USP family, and is involved in cleaving and removing ubiquitin or ubiquitin-like molecules from their target molecules. Recently, increasing evidence has suggested that USP18 is constitutively expressed in different types of human tumors, and ectopic expression or downregulation of USP18 expression may contribute to tumorigenesis. However, the role of USP18 in uterine cervical cancer (UCC) remains unclear. Thus, the present study aimed to investigate USP18 expression in a human tissue microarray constructed using UCC and non-cancer cervical tissues, and to determine the potential role and molecular mechanism by which USP18 is implicated in the tumor biology of human UCC HeLa cells. Microarray analysis demonstrated that USP18 protein expression was downregulated in tumor tissues compared with in normal tissues. In addition, in vitro analysis revealed that USP18-knockdown markedly promoted the proliferation, colony formation, migration and aggressiveness of HeLa cells. Mechanistic analysis demonstrated that USP18-knockdown increased the levels of Bcl-2, STAT3 and phosphorylated-ERK in HeLa cells. Notably, USP18 silencing-induced malignant phenotypes were interrupted following exogenous administration of the ERK1/2 inhibitor PD98059. Overall, the results of the present study suggested that USP18 may be a potent inhibitor involved in UCC tumor-associated biological behaviors, which are associated with the ERK signaling pathway. |
format | Online Article Text |
id | pubmed-8025074 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-80250742021-04-12 USP18-deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal-dependent manner Pan, Aonan Li, Yue Guan, Jian Zhang, Pengxia Zhang, Chunbin Han, Yupeng Zhang, Tao Cheng, Yao Sun, Luo Lu, Shizhen Weng, Jinru Ren, Qiaosheng Fan, Shengjie Wang, Weiqun Wang, Jingtao Oncol Lett Articles Ubiquitin-specific peptidase (USP)18 belongs to the USP family, and is involved in cleaving and removing ubiquitin or ubiquitin-like molecules from their target molecules. Recently, increasing evidence has suggested that USP18 is constitutively expressed in different types of human tumors, and ectopic expression or downregulation of USP18 expression may contribute to tumorigenesis. However, the role of USP18 in uterine cervical cancer (UCC) remains unclear. Thus, the present study aimed to investigate USP18 expression in a human tissue microarray constructed using UCC and non-cancer cervical tissues, and to determine the potential role and molecular mechanism by which USP18 is implicated in the tumor biology of human UCC HeLa cells. Microarray analysis demonstrated that USP18 protein expression was downregulated in tumor tissues compared with in normal tissues. In addition, in vitro analysis revealed that USP18-knockdown markedly promoted the proliferation, colony formation, migration and aggressiveness of HeLa cells. Mechanistic analysis demonstrated that USP18-knockdown increased the levels of Bcl-2, STAT3 and phosphorylated-ERK in HeLa cells. Notably, USP18 silencing-induced malignant phenotypes were interrupted following exogenous administration of the ERK1/2 inhibitor PD98059. Overall, the results of the present study suggested that USP18 may be a potent inhibitor involved in UCC tumor-associated biological behaviors, which are associated with the ERK signaling pathway. D.A. Spandidos 2021-05 2021-03-29 /pmc/articles/PMC8025074/ /pubmed/33850562 http://dx.doi.org/10.3892/ol.2021.12682 Text en Copyright: © Pan et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Pan, Aonan Li, Yue Guan, Jian Zhang, Pengxia Zhang, Chunbin Han, Yupeng Zhang, Tao Cheng, Yao Sun, Luo Lu, Shizhen Weng, Jinru Ren, Qiaosheng Fan, Shengjie Wang, Weiqun Wang, Jingtao USP18-deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal-dependent manner |
title | USP18-deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal-dependent manner |
title_full | USP18-deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal-dependent manner |
title_fullStr | USP18-deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal-dependent manner |
title_full_unstemmed | USP18-deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal-dependent manner |
title_short | USP18-deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an ERK signal-dependent manner |
title_sort | usp18-deficiency in cervical carcinoma is crucial for the malignant behavior of tumor cells in an erk signal-dependent manner |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025074/ https://www.ncbi.nlm.nih.gov/pubmed/33850562 http://dx.doi.org/10.3892/ol.2021.12682 |
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