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Recombinant unpurified rETX(H106P)/CTB-rETX(Y196E) protects rabbits against Clostridium perfringens epsilon toxin
Epsilon toxin (ETX), produced by Clostridium perfringens types B and D, has been touted as a potential biological weapon and is known to induce fatal enterotoxemia in a variety of livestock animals. For the efficient production of recombinant proteins with the objective of investigating the effects...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Japanese Society of Veterinary Science
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025428/ https://www.ncbi.nlm.nih.gov/pubmed/33551442 http://dx.doi.org/10.1292/jvms.20-0385 |
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author | PENG, Xiaobing LI, Xuni PENG, Guorui FENG, Lifang JIANG, Yuwen LUO, Yufeng |
author_facet | PENG, Xiaobing LI, Xuni PENG, Guorui FENG, Lifang JIANG, Yuwen LUO, Yufeng |
author_sort | PENG, Xiaobing |
collection | PubMed |
description | Epsilon toxin (ETX), produced by Clostridium perfringens types B and D, has been touted as a potential biological weapon and is known to induce fatal enterotoxemia in a variety of livestock animals. For the efficient production of recombinant proteins with the objective of investigating the effects of different recombinant vaccines against ETX, a bicistronic design (BCD) expression system including the ETX coding sequence with mutation of amino acid 106 from Histidine to Proline (ETX(H106P)) in the first cistron, followed by Cholera Toxin B (CTB) linked with the ETX coding sequence with mutation of amino acid 196 from Tyrosine to Glutamic acid (ETX(Y196E)) in the second cistron, was generated under the control of a single promoter. Rabbits were immunized twice with five inactivated recombinant Escherichia coli (E. coli) vaccines containing 100 µg/ml of the recombinant mutant rETX(H106P)/CTB-rETX(Y196E) proteins mixed with different adjuvants. Apart from rETX(H106P)/CTB-rETX(Y196E)-IMS1313-vaccinated rabbits, the neutralizing antibody titers of rETX(H106P)/CTB-rETX(Y196E)-vaccinated rabbits were higher after the initial immunization than those administered the ETX toxoid or current commercial vaccines. rETX(H106P)/CTB-rETX(Y196E) mixed with ISA201 induced the highest neutralizing antibody titer of 120 after the first immunization, suggesting that 0.1 ml of pooled sera could neutralize 120× mouse LD(100) (100% lethal dose) of ETX. Following the second vaccination, rETX(H106P)/CTB-rETX(Y196E) mixed with ISA201 or GR208 produced the highest neutralizing titer of 800. Rabbits from all vaccinated groups were completely protected from a 2× rabbit LD(100) of ETX challenge. These results show that these novel recombinant proteins can induce a strong immune response and represent potential targets for the development of a commercial vaccine against the C. perfringens epsilon toxin. |
format | Online Article Text |
id | pubmed-8025428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-80254282021-04-13 Recombinant unpurified rETX(H106P)/CTB-rETX(Y196E) protects rabbits against Clostridium perfringens epsilon toxin PENG, Xiaobing LI, Xuni PENG, Guorui FENG, Lifang JIANG, Yuwen LUO, Yufeng J Vet Med Sci Immunology Epsilon toxin (ETX), produced by Clostridium perfringens types B and D, has been touted as a potential biological weapon and is known to induce fatal enterotoxemia in a variety of livestock animals. For the efficient production of recombinant proteins with the objective of investigating the effects of different recombinant vaccines against ETX, a bicistronic design (BCD) expression system including the ETX coding sequence with mutation of amino acid 106 from Histidine to Proline (ETX(H106P)) in the first cistron, followed by Cholera Toxin B (CTB) linked with the ETX coding sequence with mutation of amino acid 196 from Tyrosine to Glutamic acid (ETX(Y196E)) in the second cistron, was generated under the control of a single promoter. Rabbits were immunized twice with five inactivated recombinant Escherichia coli (E. coli) vaccines containing 100 µg/ml of the recombinant mutant rETX(H106P)/CTB-rETX(Y196E) proteins mixed with different adjuvants. Apart from rETX(H106P)/CTB-rETX(Y196E)-IMS1313-vaccinated rabbits, the neutralizing antibody titers of rETX(H106P)/CTB-rETX(Y196E)-vaccinated rabbits were higher after the initial immunization than those administered the ETX toxoid or current commercial vaccines. rETX(H106P)/CTB-rETX(Y196E) mixed with ISA201 induced the highest neutralizing antibody titer of 120 after the first immunization, suggesting that 0.1 ml of pooled sera could neutralize 120× mouse LD(100) (100% lethal dose) of ETX. Following the second vaccination, rETX(H106P)/CTB-rETX(Y196E) mixed with ISA201 or GR208 produced the highest neutralizing titer of 800. Rabbits from all vaccinated groups were completely protected from a 2× rabbit LD(100) of ETX challenge. These results show that these novel recombinant proteins can induce a strong immune response and represent potential targets for the development of a commercial vaccine against the C. perfringens epsilon toxin. The Japanese Society of Veterinary Science 2021-02-08 2021-03 /pmc/articles/PMC8025428/ /pubmed/33551442 http://dx.doi.org/10.1292/jvms.20-0385 Text en ©2021 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Immunology PENG, Xiaobing LI, Xuni PENG, Guorui FENG, Lifang JIANG, Yuwen LUO, Yufeng Recombinant unpurified rETX(H106P)/CTB-rETX(Y196E) protects rabbits against Clostridium perfringens epsilon toxin |
title | Recombinant unpurified rETX(H106P)/CTB-rETX(Y196E) protects rabbits against Clostridium perfringens epsilon
toxin |
title_full | Recombinant unpurified rETX(H106P)/CTB-rETX(Y196E) protects rabbits against Clostridium perfringens epsilon
toxin |
title_fullStr | Recombinant unpurified rETX(H106P)/CTB-rETX(Y196E) protects rabbits against Clostridium perfringens epsilon
toxin |
title_full_unstemmed | Recombinant unpurified rETX(H106P)/CTB-rETX(Y196E) protects rabbits against Clostridium perfringens epsilon
toxin |
title_short | Recombinant unpurified rETX(H106P)/CTB-rETX(Y196E) protects rabbits against Clostridium perfringens epsilon
toxin |
title_sort | recombinant unpurified retx(h106p)/ctb-retx(y196e) protects rabbits against clostridium perfringens epsilon
toxin |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025428/ https://www.ncbi.nlm.nih.gov/pubmed/33551442 http://dx.doi.org/10.1292/jvms.20-0385 |
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