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Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi –proven vector of zoonotic cutaneous leishmaniasis in Iran

BACKGROUND: Zoonotic Cutaneous Leishmaniasis is increasing in the world and Phlebotomus papatasi as a proven vector was considered in different aspects for disease control. Sandfly saliva contains proteins which provoke host immune system. These proteins are candidates for developing vaccines. OBJEC...

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Autores principales: Maryam Ghafari, Seyedeh, Ebrahimi, Sahar, Nateghi Rostami, Mahmoud, Bordbar, Ali, Parvizi, Parviz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025609/
https://www.ncbi.nlm.nih.gov/pubmed/32969601
http://dx.doi.org/10.1002/vms3.368
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author Maryam Ghafari, Seyedeh
Ebrahimi, Sahar
Nateghi Rostami, Mahmoud
Bordbar, Ali
Parvizi, Parviz
author_facet Maryam Ghafari, Seyedeh
Ebrahimi, Sahar
Nateghi Rostami, Mahmoud
Bordbar, Ali
Parvizi, Parviz
author_sort Maryam Ghafari, Seyedeh
collection PubMed
description BACKGROUND: Zoonotic Cutaneous Leishmaniasis is increasing in the world and Phlebotomus papatasi as a proven vector was considered in different aspects for disease control. Sandfly saliva contains proteins which provoke host immune system. These proteins are candidates for developing vaccines. OBJECTIVES: The main purpose of this research was comparing evaluation of salivary glands proteomes from wild P. papatasi. Extracting these proteins and purifying of original SP15 as inducer agent in vector salivary glands from endemic leishmaniasis foci were other objectives. METHODS: Adult sandflies were sampled using aspirators and funnel traps from three endemic foci in 2017–2018. Each pair of salivary glands of unfed females was dissected and proteins were extracted using thermal shocking and sonication methods. Purification was performed through RP‐HPLC. All equivalent fractions were added together in order to reach sufficient protein concentration. Protein content and profile determination were examined with SDS‐PAGE. RESULTS: The protein concentration of whole‐salivary glands of specimens was determined approximately 1.6 µg/µl (Isfahan) and 1 µg/µl (Varamin and Kashan). SDS‐PAGE revealed 10 distinct bands between 10 and 63 kDa. Analysis of proteomes showed some similarities and differences in the chromatograms of different foci. SDS‐PAGE of all collected fractions revealed SP15‐like proteins were isolated in 24 min from Varamin, 26 to 30 min from Kashan and 29.4 min from Isfahan and were around 15 kDa. CONCLUSIONS: Isolation of salivary components of Iranian wild P. papatasi is very important for finding potential proteins in vaccine development and measuring control strategy of zoonotic cutaneous leishmaniasis in Iran and this could be concluded elsewhere in the world.
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spelling pubmed-80256092021-04-13 Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi –proven vector of zoonotic cutaneous leishmaniasis in Iran Maryam Ghafari, Seyedeh Ebrahimi, Sahar Nateghi Rostami, Mahmoud Bordbar, Ali Parvizi, Parviz Vet Med Sci Original Articles BACKGROUND: Zoonotic Cutaneous Leishmaniasis is increasing in the world and Phlebotomus papatasi as a proven vector was considered in different aspects for disease control. Sandfly saliva contains proteins which provoke host immune system. These proteins are candidates for developing vaccines. OBJECTIVES: The main purpose of this research was comparing evaluation of salivary glands proteomes from wild P. papatasi. Extracting these proteins and purifying of original SP15 as inducer agent in vector salivary glands from endemic leishmaniasis foci were other objectives. METHODS: Adult sandflies were sampled using aspirators and funnel traps from three endemic foci in 2017–2018. Each pair of salivary glands of unfed females was dissected and proteins were extracted using thermal shocking and sonication methods. Purification was performed through RP‐HPLC. All equivalent fractions were added together in order to reach sufficient protein concentration. Protein content and profile determination were examined with SDS‐PAGE. RESULTS: The protein concentration of whole‐salivary glands of specimens was determined approximately 1.6 µg/µl (Isfahan) and 1 µg/µl (Varamin and Kashan). SDS‐PAGE revealed 10 distinct bands between 10 and 63 kDa. Analysis of proteomes showed some similarities and differences in the chromatograms of different foci. SDS‐PAGE of all collected fractions revealed SP15‐like proteins were isolated in 24 min from Varamin, 26 to 30 min from Kashan and 29.4 min from Isfahan and were around 15 kDa. CONCLUSIONS: Isolation of salivary components of Iranian wild P. papatasi is very important for finding potential proteins in vaccine development and measuring control strategy of zoonotic cutaneous leishmaniasis in Iran and this could be concluded elsewhere in the world. John Wiley and Sons Inc. 2020-09-24 /pmc/articles/PMC8025609/ /pubmed/32969601 http://dx.doi.org/10.1002/vms3.368 Text en © 2020 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Maryam Ghafari, Seyedeh
Ebrahimi, Sahar
Nateghi Rostami, Mahmoud
Bordbar, Ali
Parvizi, Parviz
Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi –proven vector of zoonotic cutaneous leishmaniasis in Iran
title Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi –proven vector of zoonotic cutaneous leishmaniasis in Iran
title_full Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi –proven vector of zoonotic cutaneous leishmaniasis in Iran
title_fullStr Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi –proven vector of zoonotic cutaneous leishmaniasis in Iran
title_full_unstemmed Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi –proven vector of zoonotic cutaneous leishmaniasis in Iran
title_short Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi –proven vector of zoonotic cutaneous leishmaniasis in Iran
title_sort comparative evaluation of salivary glands proteomes from wild phlebotomus papatasi –proven vector of zoonotic cutaneous leishmaniasis in iran
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025609/
https://www.ncbi.nlm.nih.gov/pubmed/32969601
http://dx.doi.org/10.1002/vms3.368
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