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Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi –proven vector of zoonotic cutaneous leishmaniasis in Iran
BACKGROUND: Zoonotic Cutaneous Leishmaniasis is increasing in the world and Phlebotomus papatasi as a proven vector was considered in different aspects for disease control. Sandfly saliva contains proteins which provoke host immune system. These proteins are candidates for developing vaccines. OBJEC...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025609/ https://www.ncbi.nlm.nih.gov/pubmed/32969601 http://dx.doi.org/10.1002/vms3.368 |
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author | Maryam Ghafari, Seyedeh Ebrahimi, Sahar Nateghi Rostami, Mahmoud Bordbar, Ali Parvizi, Parviz |
author_facet | Maryam Ghafari, Seyedeh Ebrahimi, Sahar Nateghi Rostami, Mahmoud Bordbar, Ali Parvizi, Parviz |
author_sort | Maryam Ghafari, Seyedeh |
collection | PubMed |
description | BACKGROUND: Zoonotic Cutaneous Leishmaniasis is increasing in the world and Phlebotomus papatasi as a proven vector was considered in different aspects for disease control. Sandfly saliva contains proteins which provoke host immune system. These proteins are candidates for developing vaccines. OBJECTIVES: The main purpose of this research was comparing evaluation of salivary glands proteomes from wild P. papatasi. Extracting these proteins and purifying of original SP15 as inducer agent in vector salivary glands from endemic leishmaniasis foci were other objectives. METHODS: Adult sandflies were sampled using aspirators and funnel traps from three endemic foci in 2017–2018. Each pair of salivary glands of unfed females was dissected and proteins were extracted using thermal shocking and sonication methods. Purification was performed through RP‐HPLC. All equivalent fractions were added together in order to reach sufficient protein concentration. Protein content and profile determination were examined with SDS‐PAGE. RESULTS: The protein concentration of whole‐salivary glands of specimens was determined approximately 1.6 µg/µl (Isfahan) and 1 µg/µl (Varamin and Kashan). SDS‐PAGE revealed 10 distinct bands between 10 and 63 kDa. Analysis of proteomes showed some similarities and differences in the chromatograms of different foci. SDS‐PAGE of all collected fractions revealed SP15‐like proteins were isolated in 24 min from Varamin, 26 to 30 min from Kashan and 29.4 min from Isfahan and were around 15 kDa. CONCLUSIONS: Isolation of salivary components of Iranian wild P. papatasi is very important for finding potential proteins in vaccine development and measuring control strategy of zoonotic cutaneous leishmaniasis in Iran and this could be concluded elsewhere in the world. |
format | Online Article Text |
id | pubmed-8025609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80256092021-04-13 Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi –proven vector of zoonotic cutaneous leishmaniasis in Iran Maryam Ghafari, Seyedeh Ebrahimi, Sahar Nateghi Rostami, Mahmoud Bordbar, Ali Parvizi, Parviz Vet Med Sci Original Articles BACKGROUND: Zoonotic Cutaneous Leishmaniasis is increasing in the world and Phlebotomus papatasi as a proven vector was considered in different aspects for disease control. Sandfly saliva contains proteins which provoke host immune system. These proteins are candidates for developing vaccines. OBJECTIVES: The main purpose of this research was comparing evaluation of salivary glands proteomes from wild P. papatasi. Extracting these proteins and purifying of original SP15 as inducer agent in vector salivary glands from endemic leishmaniasis foci were other objectives. METHODS: Adult sandflies were sampled using aspirators and funnel traps from three endemic foci in 2017–2018. Each pair of salivary glands of unfed females was dissected and proteins were extracted using thermal shocking and sonication methods. Purification was performed through RP‐HPLC. All equivalent fractions were added together in order to reach sufficient protein concentration. Protein content and profile determination were examined with SDS‐PAGE. RESULTS: The protein concentration of whole‐salivary glands of specimens was determined approximately 1.6 µg/µl (Isfahan) and 1 µg/µl (Varamin and Kashan). SDS‐PAGE revealed 10 distinct bands between 10 and 63 kDa. Analysis of proteomes showed some similarities and differences in the chromatograms of different foci. SDS‐PAGE of all collected fractions revealed SP15‐like proteins were isolated in 24 min from Varamin, 26 to 30 min from Kashan and 29.4 min from Isfahan and were around 15 kDa. CONCLUSIONS: Isolation of salivary components of Iranian wild P. papatasi is very important for finding potential proteins in vaccine development and measuring control strategy of zoonotic cutaneous leishmaniasis in Iran and this could be concluded elsewhere in the world. John Wiley and Sons Inc. 2020-09-24 /pmc/articles/PMC8025609/ /pubmed/32969601 http://dx.doi.org/10.1002/vms3.368 Text en © 2020 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Maryam Ghafari, Seyedeh Ebrahimi, Sahar Nateghi Rostami, Mahmoud Bordbar, Ali Parvizi, Parviz Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi –proven vector of zoonotic cutaneous leishmaniasis in Iran |
title | Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi
–proven vector of zoonotic cutaneous leishmaniasis in Iran |
title_full | Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi
–proven vector of zoonotic cutaneous leishmaniasis in Iran |
title_fullStr | Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi
–proven vector of zoonotic cutaneous leishmaniasis in Iran |
title_full_unstemmed | Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi
–proven vector of zoonotic cutaneous leishmaniasis in Iran |
title_short | Comparative evaluation of salivary glands proteomes from wild Phlebotomus papatasi
–proven vector of zoonotic cutaneous leishmaniasis in Iran |
title_sort | comparative evaluation of salivary glands proteomes from wild phlebotomus papatasi
–proven vector of zoonotic cutaneous leishmaniasis in iran |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025609/ https://www.ncbi.nlm.nih.gov/pubmed/32969601 http://dx.doi.org/10.1002/vms3.368 |
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