Cargando…

A rapid and simple single‐step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites

This study describes a simple method for the large‐scale isolation of pure Toxoplasma gondii tachyzoites and bradyzoites. T. gondii tachyzoites were obtained from infected human foreskin fibroblasts (HFFs) and peritoneal exudates of mice, while tissue cysts containing bradyzoites were collected from...

Descripción completa

Detalles Bibliográficos
Autores principales:	El‐Ashram, Saeed, Zhang, Yu, Ji, Yongsheng, Salama, Dina, Mei, Kun, Zhili, Li, Shujian, Huang, Zhang, Haoji, Aboelhadid, Shawky M., Alajmi, Reem A., Metwally, Dina M., El‐Khadragy, Manal F., Hargis, Billy M., Tellez‐Isaias, Guillermo, Cenci‐Goga, Beniamino T., Karama, Musafiri, Marufu, Munyaradzi C., Abouhajer, Fathi, Ali Abdelhafez Hamady, Gamal, El Wakil, Abeer, Al Nasr, Ibrahim, Suo, Xun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2020
Materias:	Original Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025613/ https://www.ncbi.nlm.nih.gov/pubmed/32979302 http://dx.doi.org/10.1002/vms3.364

Ejemplares similares

Identification of compounds that suppress Toxoplasma gondii tachyzoites and bradyzoites
por: Murata, Yuho, et al.
Publicado: (2017)

Nanosilver Colloid Inhibits Toxoplasma gondii Tachyzoites and Bradyzoites in Vitro
por: SHOJAEE, Saeedeh, et al.
Publicado: (2019)

Toxoplasma gondii bradyzoites and tachyzoites isolation from vitreous of atypical necrotizing retinitis
por: Kharel(Sitaula), Ranju, et al.
Publicado: (2018)

Molecular profiling and antimicrobial resistance of Shiga toxin-producing Escherichia coli O26, O45, O103, O121, O145 and O157 isolates from cattle on cow-calf operations in South Africa
por: Karama, Musafiri, et al.
Publicado: (2019)

Occurrence, Serotypes and Virulence Characteristics of Shiga-Toxin-Producing Escherichia coli Isolates from Goats on Communal Rangeland in South Africa
por: Malahlela, Mogaugedi N., et al.
Publicado: (2022)

Evolution and antimicrobial resistance of enterococci isolated from Pecorino and goat cheese manufactured on-farm in an area facing constraints as per EU Regulation 1305/2013 in Umbria, Italy
por: Grispoldi, Luca, et al.
Publicado: (2022)

TgLaforin, a glucan phosphatase, reveals the dynamic role of storage polysaccharides in Toxoplasma gondii tachyzoites and bradyzoites
por: Murphy, Robert D., et al.
Publicado: (2023)

Silver Nanoparticles Biosynthesized With Salvia officinalis Leaf Exert Protective Effect on Hepatic Tissue Injury Induced by Plasmodium chabaudi
por: Metwally, Dina M., et al.
Publicado: (2021)

Toxoplasma gondii Cyclic AMP-Dependent Protein Kinase Subunit 3 Is Involved in the Switch from Tachyzoite to Bradyzoite Development
por: Sugi, Tatsuki, et al.
Publicado: (2016)

Molecular identification of Campanulotes bidentatus Scopoli, 1763 (Phthiraptera, Philopteridae) infecting the domestic pigeon Columba livia from Saudi Arabia
por: Alajmi, Reem A., et al.
Publicado: (2021)

Prevalence and risk factors associated with Campylobacter spp. occurrence in healthy dogs visiting four rural community veterinary clinics in South Africa
por: Karama, Musafiri, et al.
Publicado: (2019)

Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques
por: Metwally, Dina M., et al.
Publicado: (2021)

Virulence Characteristics and Antimicrobial Resistance Profiles of Shiga Toxin-Producing Escherichia coli Isolates from Humans in South Africa: 2006–2013
por: Karama, Musafiri, et al.
Publicado: (2019)

Prevalence of eimeriosis in the one-humped camels (Camelus dromedarius) from Riyadh and Al-Qassim, Saudi Arabia
por: Metwally, Dina M., et al.
Publicado: (2020)

Effectiveness of naturally occurring Aphis gossypii on tomato plants as a bio-indicator for heavy metals in Riyadh and Hafar Al-Batin, Saudi Arabia
por: Alajmi, Reem, et al.
Publicado: (2021)

Identification of Sarcocystis spp. in One-humped Camels (Camelus dromedarius) from Riyadh and Dammam, Saudi Arabia, via Histological and Phylogenetic Approaches
por: Metwally, Dina M., et al.
Publicado: (2020)

Molecular diagnosis of acute and chronic infection of Trypanosoma evansi in experimental male and female mice
por: Behour, Tahani S., et al.
Publicado: (2019)

Effect of packaging and storage conditions on some quality traits of bovine meat
por: Grispoldi, Luca, et al.
Publicado: (2022)

Diversity of Parasitic Diarrhea Associated with Buxtonella Sulcata in Cattle and Buffalo Calves with Control of Buxtonellosis
por: El-Ashram, Saeed, et al.
Publicado: (2019)

First Report of Cattle Tick Rhipicephalus (Boophilus) annulatus in Egypt Resistant to Ivermectin
por: El-Ashram, Saeed, et al.
Publicado: (2019)

Anti-Leishmanial Activity (In Vitro and In Vivo) of Allicin and Allicin Cream Using Leishmania major (Sub-strain Zymowme LON4) and Balb/c Mice
por: Metwally, Dina M., et al.
Publicado: (2016)

Erratum: Saeed, E.-A.; Shawky, M.A.; El-Sayed, M.A.-K.; Shymaa, A.H.; Lilian, N.M.; Eman, M.F.; Asmaa, A.K. Investigation of Pre- and Post-Weaning Mortalities in Rabbits Bred in Egypt, with Reference to Parasitic and Bacterial Causes. Animals 2020, 10, 537
por: El-Ashram, Saeed, et al.
Publicado: (2020)

Investigation of Pre- and Post-Weaning Mortalities in Rabbits Bred in Egypt, with Reference to Parasitic and Bacterial Causes
por: El-Ashram, Saeed, et al.
Publicado: (2020)

In Vitro and In Vivo Control of Secondary Bacterial Infection Caused by Leishmania major
por: Yehia, Hany M., et al.
Publicado: (2017)

New insight on some selected nanoparticles as an effective adsorbent toward diminishing the health risk of deltamethrin contaminated water
por: Ibrahium, Samar M., et al.
Publicado: (2021)

Protective effects of pomegranate (Punica granatum) juice on testes against carbon tetrachloride intoxication in rats
por: Al-Olayan, Ebtesam M, et al.
Publicado: (2014)

Molecular Characterization of Sarcocystis Species Isolated from Sheep and Goats in Riyadh, Saudi Arabia
por: Metwally, Dina M., et al.
Publicado: (2019)

Effects of Eugenol on Haemoproteus columbae in domestic pigeons (Columba livia domestica) from Riyadh, Saudi Arabia
por: Metwally, Dina M., et al.
Publicado: (2019)

Attenuation Effect of UV on Haemonchus contortus Larvae in Experimentally Infected Goats
por: ABOELHADID, Aboelhadid Shawky Mohamed, et al.
Publicado: (2013)

Gene-based molecular analysis of COX1 in Echinococcus granulosus cysts isolated from naturally infected livestock in Riyadh, Saudi Arabia
por: Metwally, Dina M., et al.
Publicado: (2018)

The determinants regulating Toxoplasma gondii bradyzoite development
por: Pan, Ming, et al.
Publicado: (2022)

Anti-Toxoplasma activity of silver nanoparticles green synthesized with Phoenix dactylifera and Ziziphus spina-christi extracts which inhibits inflammation through liver regulation of cytokines in Balb/c mice
por: Alajmi, Reem A., et al.
Publicado: (2019)

Prophylactic and Therapeutic Efficacy of Prebiotic Supplementation against Intestinal Coccidiosis in Rabbits
por: El-Ashram, Saeed A., et al.
Publicado: (2019)

Protective Effect of Litchi chinensis Peel Extract-Prepared Nanoparticles on Rabbits Experimentally Infected with Eimeria stiedae
por: Metwally, Dina M., et al.
Publicado: (2022)

Effect of Delayed Refrigeration on the Microbial Carcass Contamination of Wild Boars (Sus scrofa)
por: Cenci-Goga, Beniamino, et al.
Publicado: (2021)

Acquisition of Host Cytosolic Protein by Toxoplasma gondii Bradyzoites
por: Kannan, Geetha, et al.
Publicado: (2021)

Restriction Checkpoint Controls Bradyzoite Development in Toxoplasma gondii
por: Naumov, Anatoli V., et al.
Publicado: (2022)

Invasion of Toxoplasma gondii bradyzoites: Molecular dissection of the moving junction proteins and effective vaccination targets
por: Najm, Rania, et al.
Publicado: (2023)

ANK1 and DnaK-TPR, Two Tetratricopeptide Repeat-Containing Proteins Primarily Expressed in Toxoplasma Bradyzoites, Do Not Contribute to Bradyzoite Differentiation
por: Yang, Jichao, et al.
Publicado: (2017)

Prevalence Rate and Molecular Characteristics of Oestrus ovis L. (Diptera, Oestridae) in Sheep and Goats from Riyadh, Saudi Arabia
por: Metwally, Dina M., et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_gn8c4amv6vrs42ieomvcuf1ge1