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Exosomes containing miR‐451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury

AIM: To study the role of exosomes in the protective effect of cerebral ischemic preconditioning (cerebral‐IPC) against cerebral I/R injury. METHOD: Mouse models of cerebral‐IPC and MCAO/R were established as described previously, and their behavioral, pathological, and proteomic changes were analyz...

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Autores principales: Li, He, Luo, Yin, Liu, Peng, Liu, Pei, Hua, Weilong, Zhang, Yongxin, Zhang, Lei, Li, Zifu, Xing, Pengfei, Zhang, Yongwei, Hong, Bo, Yang, Pengfei, Liu, Jianmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025619/
https://www.ncbi.nlm.nih.gov/pubmed/33533575
http://dx.doi.org/10.1111/cns.13612
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author Li, He
Luo, Yin
Liu, Peng
Liu, Pei
Hua, Weilong
Zhang, Yongxin
Zhang, Lei
Li, Zifu
Xing, Pengfei
Zhang, Yongwei
Hong, Bo
Yang, Pengfei
Liu, Jianmin
author_facet Li, He
Luo, Yin
Liu, Peng
Liu, Pei
Hua, Weilong
Zhang, Yongxin
Zhang, Lei
Li, Zifu
Xing, Pengfei
Zhang, Yongwei
Hong, Bo
Yang, Pengfei
Liu, Jianmin
author_sort Li, He
collection PubMed
description AIM: To study the role of exosomes in the protective effect of cerebral ischemic preconditioning (cerebral‐IPC) against cerebral I/R injury. METHOD: Mouse models of cerebral‐IPC and MCAO/R were established as described previously, and their behavioral, pathological, and proteomic changes were analyzed. Neuro‐2a subjected to OGD/R were treated with exosomes isolated from the plasma of sham‐operated and cerebral‐IPC mice. The differentially expressed miRNAs between exosomes derived from sham‐operated (S‐exosomes) and preconditioned (IPC‐exosomes) mice were identified through miRNA array, and their targets were identified through database search. The control and OGD/R cells were treated with the IPC‐exosomes, miRNA mimic or target protein inhibitor, and their viability, oxidative, stress and apoptosis rates were measured. The activated pathways were identified by analyzing the levels of relevant proteins. RESULTS: Cerebral‐IPC mitigated the cerebral injury following ischemia and reperfusion, and increased the number of plasma exosomes. IPC‐exosomes increased the survival of Neuro‐2a cells after OGD/R. The miR‐451a targeting Rac1 was upregulated in the IPC‐exosomes relative to S‐exosomes. The miR‐451a mimic and the Rac1 inhibitor NSC23766 reversed OGD/R‐mediated activation of Rac1 and its downstream pathways. CONCLUSION: Cerebral‐IPC ameliorated cerebral I/R injury by inducing the release of exosomes containing miR‐451a.
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spelling pubmed-80256192021-04-13 Exosomes containing miR‐451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury Li, He Luo, Yin Liu, Peng Liu, Pei Hua, Weilong Zhang, Yongxin Zhang, Lei Li, Zifu Xing, Pengfei Zhang, Yongwei Hong, Bo Yang, Pengfei Liu, Jianmin CNS Neurosci Ther Original Articles AIM: To study the role of exosomes in the protective effect of cerebral ischemic preconditioning (cerebral‐IPC) against cerebral I/R injury. METHOD: Mouse models of cerebral‐IPC and MCAO/R were established as described previously, and their behavioral, pathological, and proteomic changes were analyzed. Neuro‐2a subjected to OGD/R were treated with exosomes isolated from the plasma of sham‐operated and cerebral‐IPC mice. The differentially expressed miRNAs between exosomes derived from sham‐operated (S‐exosomes) and preconditioned (IPC‐exosomes) mice were identified through miRNA array, and their targets were identified through database search. The control and OGD/R cells were treated with the IPC‐exosomes, miRNA mimic or target protein inhibitor, and their viability, oxidative, stress and apoptosis rates were measured. The activated pathways were identified by analyzing the levels of relevant proteins. RESULTS: Cerebral‐IPC mitigated the cerebral injury following ischemia and reperfusion, and increased the number of plasma exosomes. IPC‐exosomes increased the survival of Neuro‐2a cells after OGD/R. The miR‐451a targeting Rac1 was upregulated in the IPC‐exosomes relative to S‐exosomes. The miR‐451a mimic and the Rac1 inhibitor NSC23766 reversed OGD/R‐mediated activation of Rac1 and its downstream pathways. CONCLUSION: Cerebral‐IPC ameliorated cerebral I/R injury by inducing the release of exosomes containing miR‐451a. John Wiley and Sons Inc. 2021-02-03 /pmc/articles/PMC8025619/ /pubmed/33533575 http://dx.doi.org/10.1111/cns.13612 Text en © 2021 The Authors. CNS Neuroscience & Therapeutics Published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Li, He
Luo, Yin
Liu, Peng
Liu, Pei
Hua, Weilong
Zhang, Yongxin
Zhang, Lei
Li, Zifu
Xing, Pengfei
Zhang, Yongwei
Hong, Bo
Yang, Pengfei
Liu, Jianmin
Exosomes containing miR‐451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury
title Exosomes containing miR‐451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury
title_full Exosomes containing miR‐451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury
title_fullStr Exosomes containing miR‐451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury
title_full_unstemmed Exosomes containing miR‐451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury
title_short Exosomes containing miR‐451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury
title_sort exosomes containing mir‐451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025619/
https://www.ncbi.nlm.nih.gov/pubmed/33533575
http://dx.doi.org/10.1111/cns.13612
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