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Dissecting Murine Muscle Stem Cell Aging through Regeneration Using Integrative Genomic Analysis
During aging, there is a progressive loss of volume and function in skeletal muscle that impacts mobility and quality of life. The repair of skeletal muscle is regulated by tissue-resident stem cells called satellite cells (or muscle stem cells [MuSCs]), but in aging, MuSCs decrease in numbers and r...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025697/ https://www.ncbi.nlm.nih.gov/pubmed/32726628 http://dx.doi.org/10.1016/j.celrep.2020.107964 |
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author | Shcherbina, Anna Larouche, Jacqueline Fraczek, Paula Yang, Benjamin A. Brown, Lemuel A. Markworth, James F. Chung, Carolina H. Khaliq, Mehwish de Silva, Kanishka Choi, Jeongmoon J. Fallahi-Sichani, Mohammad Chandrasekaran, Sriram Jang, Young C. Brooks, Susan V. Aguilar, Carlos A. |
author_facet | Shcherbina, Anna Larouche, Jacqueline Fraczek, Paula Yang, Benjamin A. Brown, Lemuel A. Markworth, James F. Chung, Carolina H. Khaliq, Mehwish de Silva, Kanishka Choi, Jeongmoon J. Fallahi-Sichani, Mohammad Chandrasekaran, Sriram Jang, Young C. Brooks, Susan V. Aguilar, Carlos A. |
author_sort | Shcherbina, Anna |
collection | PubMed |
description | During aging, there is a progressive loss of volume and function in skeletal muscle that impacts mobility and quality of life. The repair of skeletal muscle is regulated by tissue-resident stem cells called satellite cells (or muscle stem cells [MuSCs]), but in aging, MuSCs decrease in numbers and regenerative capacity. The transcriptional networks and epigenetic changes that confer diminished regenerative function in MuSCs as a result of natural aging are only partially understood. Herein, we use an integrative genomics approach to profile MuSCs from young and aged animals before and after injury. Integration of these datasets reveals aging impacts multiple regulatory changes through significant differences in gene expression, metabolic flux, chromatin accessibility, and patterns of transcription factor (TF) binding activities. Collectively, these datasets facilitate a deeper understanding of the regulation tissue-resident stem cells use during aging and healing. |
format | Online Article Text |
id | pubmed-8025697 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
record_format | MEDLINE/PubMed |
spelling | pubmed-80256972021-04-07 Dissecting Murine Muscle Stem Cell Aging through Regeneration Using Integrative Genomic Analysis Shcherbina, Anna Larouche, Jacqueline Fraczek, Paula Yang, Benjamin A. Brown, Lemuel A. Markworth, James F. Chung, Carolina H. Khaliq, Mehwish de Silva, Kanishka Choi, Jeongmoon J. Fallahi-Sichani, Mohammad Chandrasekaran, Sriram Jang, Young C. Brooks, Susan V. Aguilar, Carlos A. Cell Rep Article During aging, there is a progressive loss of volume and function in skeletal muscle that impacts mobility and quality of life. The repair of skeletal muscle is regulated by tissue-resident stem cells called satellite cells (or muscle stem cells [MuSCs]), but in aging, MuSCs decrease in numbers and regenerative capacity. The transcriptional networks and epigenetic changes that confer diminished regenerative function in MuSCs as a result of natural aging are only partially understood. Herein, we use an integrative genomics approach to profile MuSCs from young and aged animals before and after injury. Integration of these datasets reveals aging impacts multiple regulatory changes through significant differences in gene expression, metabolic flux, chromatin accessibility, and patterns of transcription factor (TF) binding activities. Collectively, these datasets facilitate a deeper understanding of the regulation tissue-resident stem cells use during aging and healing. 2020-07-28 /pmc/articles/PMC8025697/ /pubmed/32726628 http://dx.doi.org/10.1016/j.celrep.2020.107964 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Shcherbina, Anna Larouche, Jacqueline Fraczek, Paula Yang, Benjamin A. Brown, Lemuel A. Markworth, James F. Chung, Carolina H. Khaliq, Mehwish de Silva, Kanishka Choi, Jeongmoon J. Fallahi-Sichani, Mohammad Chandrasekaran, Sriram Jang, Young C. Brooks, Susan V. Aguilar, Carlos A. Dissecting Murine Muscle Stem Cell Aging through Regeneration Using Integrative Genomic Analysis |
title | Dissecting Murine Muscle Stem Cell Aging through Regeneration Using Integrative Genomic Analysis |
title_full | Dissecting Murine Muscle Stem Cell Aging through Regeneration Using Integrative Genomic Analysis |
title_fullStr | Dissecting Murine Muscle Stem Cell Aging through Regeneration Using Integrative Genomic Analysis |
title_full_unstemmed | Dissecting Murine Muscle Stem Cell Aging through Regeneration Using Integrative Genomic Analysis |
title_short | Dissecting Murine Muscle Stem Cell Aging through Regeneration Using Integrative Genomic Analysis |
title_sort | dissecting murine muscle stem cell aging through regeneration using integrative genomic analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025697/ https://www.ncbi.nlm.nih.gov/pubmed/32726628 http://dx.doi.org/10.1016/j.celrep.2020.107964 |
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