Using RELION software within the Scipion framework

Scipion is a modular image-processing framework that integrates several software packages under a unified interface while taking care of file formats and conversions. Here, new developments and capabilities of the Scipion plugin for the widely used RELION software package are presented and illustrat...

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Detalles Bibliográficos
Autores principales: Sharov, Grigory, Morado, Dustin R., Carroni, Marta, de la Rosa-Trevín, José Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2021
Materias:
Ccp-EM
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025880/
https://www.ncbi.nlm.nih.gov/pubmed/33825701
http://dx.doi.org/10.1107/S2059798321001856
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author Sharov, Grigory
Morado, Dustin R.
Carroni, Marta
de la Rosa-Trevín, José Miguel
author_facet Sharov, Grigory
Morado, Dustin R.
Carroni, Marta
de la Rosa-Trevín, José Miguel
author_sort Sharov, Grigory
collection PubMed
description Scipion is a modular image-processing framework that integrates several software packages under a unified interface while taking care of file formats and conversions. Here, new developments and capabilities of the Scipion plugin for the widely used RELION software package are presented and illustrated with an image-processing pipeline for published data. The user interfaces of Scipion and RELION are compared and the key differences are highlighted, allowing this manuscript to be used as a guide for both new and experienced users of this software. Different on-the-fly image-processing options are also discussed, demonstrating the flexibility of the Scipion framework.
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spelling pubmed-80258802021-04-30 Using RELION software within the Scipion framework Sharov, Grigory Morado, Dustin R. Carroni, Marta de la Rosa-Trevín, José Miguel Acta Crystallogr D Struct Biol Ccp-EM Scipion is a modular image-processing framework that integrates several software packages under a unified interface while taking care of file formats and conversions. Here, new developments and capabilities of the Scipion plugin for the widely used RELION software package are presented and illustrated with an image-processing pipeline for published data. The user interfaces of Scipion and RELION are compared and the key differences are highlighted, allowing this manuscript to be used as a guide for both new and experienced users of this software. Different on-the-fly image-processing options are also discussed, demonstrating the flexibility of the Scipion framework. International Union of Crystallography 2021-03-30 /pmc/articles/PMC8025880/ /pubmed/33825701 http://dx.doi.org/10.1107/S2059798321001856 Text en © Sharov et al. 2021 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Ccp-EM
Sharov, Grigory
Morado, Dustin R.
Carroni, Marta
de la Rosa-Trevín, José Miguel
Using RELION software within the Scipion framework
title Using RELION software within the Scipion framework
title_full Using RELION software within the Scipion framework
title_fullStr Using RELION software within the Scipion framework
title_full_unstemmed Using RELION software within the Scipion framework
title_short Using RELION software within the Scipion framework
title_sort using relion software within the scipion framework
topic Ccp-EM
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025880/
https://www.ncbi.nlm.nih.gov/pubmed/33825701
http://dx.doi.org/10.1107/S2059798321001856
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