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Re-Evaluation of the Podosphaera tridactyla Species Complex in Australia
The Podosphaera tridactyla species complex is highly variable morphologically and causes powdery mildew on a wide range of Prunus species, including stone fruit. A taxonomic revision of the Po. tridactyla species complex in 2020 identified 12 species, seven of which were newly characterised. In orde...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025908/ https://www.ncbi.nlm.nih.gov/pubmed/33652636 http://dx.doi.org/10.3390/jof7030171 |
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author | Smith, Reannon L. May, Tom W. Kaur, Jatinder Sawbridge, Tim I. Mann, Ross C. Pascoe, Ian G. Edwards, Jacqueline |
author_facet | Smith, Reannon L. May, Tom W. Kaur, Jatinder Sawbridge, Tim I. Mann, Ross C. Pascoe, Ian G. Edwards, Jacqueline |
author_sort | Smith, Reannon L. |
collection | PubMed |
description | The Podosphaera tridactyla species complex is highly variable morphologically and causes powdery mildew on a wide range of Prunus species, including stone fruit. A taxonomic revision of the Po. tridactyla species complex in 2020 identified 12 species, seven of which were newly characterised. In order to clarify which species of this complex are present in Australia, next generation sequencing was used to isolate the fungal ITS+28S and host matK chloroplast gene regions from 56 powdery mildew specimens of stone fruit and ornamental Prunus species accessioned as Po. tridactyla or Oidium sp. in Australian reference collections. The specimens were collected in Australia, Switzerland, Italy and Korea and were collected from 1953 to 2018. Host species were confirmed using matK phylogenetic analysis, which identified that four had been misidentified as Prunus but were actually Malus prunifolia. Podosphaera species were identified using ITS+28S phylogenetic analysis, recognising three Podosphaera species on stone fruit and related ornamental Prunus hosts in Australia. These were Po. pannosa, the rose powdery mildew, and two species in the Po. tridactyla species complex: Po. ampla, which was the predominant species, and a previously unidentified species from peach, which we describe here as Po. cunningtonii. |
format | Online Article Text |
id | pubmed-8025908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-80259082021-04-08 Re-Evaluation of the Podosphaera tridactyla Species Complex in Australia Smith, Reannon L. May, Tom W. Kaur, Jatinder Sawbridge, Tim I. Mann, Ross C. Pascoe, Ian G. Edwards, Jacqueline J Fungi (Basel) Article The Podosphaera tridactyla species complex is highly variable morphologically and causes powdery mildew on a wide range of Prunus species, including stone fruit. A taxonomic revision of the Po. tridactyla species complex in 2020 identified 12 species, seven of which were newly characterised. In order to clarify which species of this complex are present in Australia, next generation sequencing was used to isolate the fungal ITS+28S and host matK chloroplast gene regions from 56 powdery mildew specimens of stone fruit and ornamental Prunus species accessioned as Po. tridactyla or Oidium sp. in Australian reference collections. The specimens were collected in Australia, Switzerland, Italy and Korea and were collected from 1953 to 2018. Host species were confirmed using matK phylogenetic analysis, which identified that four had been misidentified as Prunus but were actually Malus prunifolia. Podosphaera species were identified using ITS+28S phylogenetic analysis, recognising three Podosphaera species on stone fruit and related ornamental Prunus hosts in Australia. These were Po. pannosa, the rose powdery mildew, and two species in the Po. tridactyla species complex: Po. ampla, which was the predominant species, and a previously unidentified species from peach, which we describe here as Po. cunningtonii. MDPI 2021-02-26 /pmc/articles/PMC8025908/ /pubmed/33652636 http://dx.doi.org/10.3390/jof7030171 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Article Smith, Reannon L. May, Tom W. Kaur, Jatinder Sawbridge, Tim I. Mann, Ross C. Pascoe, Ian G. Edwards, Jacqueline Re-Evaluation of the Podosphaera tridactyla Species Complex in Australia |
title | Re-Evaluation of the Podosphaera tridactyla Species Complex in Australia |
title_full | Re-Evaluation of the Podosphaera tridactyla Species Complex in Australia |
title_fullStr | Re-Evaluation of the Podosphaera tridactyla Species Complex in Australia |
title_full_unstemmed | Re-Evaluation of the Podosphaera tridactyla Species Complex in Australia |
title_short | Re-Evaluation of the Podosphaera tridactyla Species Complex in Australia |
title_sort | re-evaluation of the podosphaera tridactyla species complex in australia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8025908/ https://www.ncbi.nlm.nih.gov/pubmed/33652636 http://dx.doi.org/10.3390/jof7030171 |
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