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5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models
Gastrointestinal stromal tumor (GIST) diagnosis using conventional gastrointestinal endoscopy is difficult because such malignancies cannot be distinguished from other types of submucosal tumors. Photodynamic diagnosis (PDD) is based on the preferential uptake of photosensitizers by tumor tissues an...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026038/ https://www.ncbi.nlm.nih.gov/pubmed/33826667 http://dx.doi.org/10.1371/journal.pone.0249650 |
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author | Sasaki, Makiko Tanaka, Mamoru Ichikawa, Hiroshi Suzuki, Taketo Nishie, Hirotada Ozeki, Keiji Shimura, Takaya Kubota, Eiji Tanida, Satoshi Kataoka, Hiromi |
author_facet | Sasaki, Makiko Tanaka, Mamoru Ichikawa, Hiroshi Suzuki, Taketo Nishie, Hirotada Ozeki, Keiji Shimura, Takaya Kubota, Eiji Tanida, Satoshi Kataoka, Hiromi |
author_sort | Sasaki, Makiko |
collection | PubMed |
description | Gastrointestinal stromal tumor (GIST) diagnosis using conventional gastrointestinal endoscopy is difficult because such malignancies cannot be distinguished from other types of submucosal tumors. Photodynamic diagnosis (PDD) is based on the preferential uptake of photosensitizers by tumor tissues and its detection by fluorescence emission upon laser excitation. In this study, we investigated whether PDD using 5-aminolevulinic acid (5-ALA), a standard photosensitizer used worldwide, could be used for GIST diagnosis. 5-ALA is metabolized to endogenous fluorescent protoporphyrin IX (PpIX). We examined the accumulation of PpIX in GIST-T1 cells using flow cytometry and immunofluorescent staining. Furthermore, we established GIST-T1 xenograft mouse models and examined PpIX accumulation in the resultant tumors. PpIX accumulated in GIST-T1 cells and was localized mainly to lysosomes. PpIX accumulation was also observed in murine xenograft tumors. Moreover, tumor and normal tissues could be distinctly identified by relative PpIX fluorescence. Thus, our results demonstrated that PDD with 5-ALA has substantial clinical potential for GIST diagnosis. |
format | Online Article Text |
id | pubmed-8026038 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-80260382021-04-15 5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models Sasaki, Makiko Tanaka, Mamoru Ichikawa, Hiroshi Suzuki, Taketo Nishie, Hirotada Ozeki, Keiji Shimura, Takaya Kubota, Eiji Tanida, Satoshi Kataoka, Hiromi PLoS One Research Article Gastrointestinal stromal tumor (GIST) diagnosis using conventional gastrointestinal endoscopy is difficult because such malignancies cannot be distinguished from other types of submucosal tumors. Photodynamic diagnosis (PDD) is based on the preferential uptake of photosensitizers by tumor tissues and its detection by fluorescence emission upon laser excitation. In this study, we investigated whether PDD using 5-aminolevulinic acid (5-ALA), a standard photosensitizer used worldwide, could be used for GIST diagnosis. 5-ALA is metabolized to endogenous fluorescent protoporphyrin IX (PpIX). We examined the accumulation of PpIX in GIST-T1 cells using flow cytometry and immunofluorescent staining. Furthermore, we established GIST-T1 xenograft mouse models and examined PpIX accumulation in the resultant tumors. PpIX accumulated in GIST-T1 cells and was localized mainly to lysosomes. PpIX accumulation was also observed in murine xenograft tumors. Moreover, tumor and normal tissues could be distinctly identified by relative PpIX fluorescence. Thus, our results demonstrated that PDD with 5-ALA has substantial clinical potential for GIST diagnosis. Public Library of Science 2021-04-07 /pmc/articles/PMC8026038/ /pubmed/33826667 http://dx.doi.org/10.1371/journal.pone.0249650 Text en © 2021 Sasaki et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Sasaki, Makiko Tanaka, Mamoru Ichikawa, Hiroshi Suzuki, Taketo Nishie, Hirotada Ozeki, Keiji Shimura, Takaya Kubota, Eiji Tanida, Satoshi Kataoka, Hiromi 5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models |
title | 5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models |
title_full | 5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models |
title_fullStr | 5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models |
title_full_unstemmed | 5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models |
title_short | 5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models |
title_sort | 5-aminolaevulinic acid (5-ala) accumulates in gist-t1 cells and photodynamic diagnosis using 5-ala identifies gastrointestinal stromal tumors (gists) in xenograft tumor models |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026038/ https://www.ncbi.nlm.nih.gov/pubmed/33826667 http://dx.doi.org/10.1371/journal.pone.0249650 |
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