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5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models

Gastrointestinal stromal tumor (GIST) diagnosis using conventional gastrointestinal endoscopy is difficult because such malignancies cannot be distinguished from other types of submucosal tumors. Photodynamic diagnosis (PDD) is based on the preferential uptake of photosensitizers by tumor tissues an...

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Autores principales: Sasaki, Makiko, Tanaka, Mamoru, Ichikawa, Hiroshi, Suzuki, Taketo, Nishie, Hirotada, Ozeki, Keiji, Shimura, Takaya, Kubota, Eiji, Tanida, Satoshi, Kataoka, Hiromi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026038/
https://www.ncbi.nlm.nih.gov/pubmed/33826667
http://dx.doi.org/10.1371/journal.pone.0249650
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author Sasaki, Makiko
Tanaka, Mamoru
Ichikawa, Hiroshi
Suzuki, Taketo
Nishie, Hirotada
Ozeki, Keiji
Shimura, Takaya
Kubota, Eiji
Tanida, Satoshi
Kataoka, Hiromi
author_facet Sasaki, Makiko
Tanaka, Mamoru
Ichikawa, Hiroshi
Suzuki, Taketo
Nishie, Hirotada
Ozeki, Keiji
Shimura, Takaya
Kubota, Eiji
Tanida, Satoshi
Kataoka, Hiromi
author_sort Sasaki, Makiko
collection PubMed
description Gastrointestinal stromal tumor (GIST) diagnosis using conventional gastrointestinal endoscopy is difficult because such malignancies cannot be distinguished from other types of submucosal tumors. Photodynamic diagnosis (PDD) is based on the preferential uptake of photosensitizers by tumor tissues and its detection by fluorescence emission upon laser excitation. In this study, we investigated whether PDD using 5-aminolevulinic acid (5-ALA), a standard photosensitizer used worldwide, could be used for GIST diagnosis. 5-ALA is metabolized to endogenous fluorescent protoporphyrin IX (PpIX). We examined the accumulation of PpIX in GIST-T1 cells using flow cytometry and immunofluorescent staining. Furthermore, we established GIST-T1 xenograft mouse models and examined PpIX accumulation in the resultant tumors. PpIX accumulated in GIST-T1 cells and was localized mainly to lysosomes. PpIX accumulation was also observed in murine xenograft tumors. Moreover, tumor and normal tissues could be distinctly identified by relative PpIX fluorescence. Thus, our results demonstrated that PDD with 5-ALA has substantial clinical potential for GIST diagnosis.
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spelling pubmed-80260382021-04-15 5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models Sasaki, Makiko Tanaka, Mamoru Ichikawa, Hiroshi Suzuki, Taketo Nishie, Hirotada Ozeki, Keiji Shimura, Takaya Kubota, Eiji Tanida, Satoshi Kataoka, Hiromi PLoS One Research Article Gastrointestinal stromal tumor (GIST) diagnosis using conventional gastrointestinal endoscopy is difficult because such malignancies cannot be distinguished from other types of submucosal tumors. Photodynamic diagnosis (PDD) is based on the preferential uptake of photosensitizers by tumor tissues and its detection by fluorescence emission upon laser excitation. In this study, we investigated whether PDD using 5-aminolevulinic acid (5-ALA), a standard photosensitizer used worldwide, could be used for GIST diagnosis. 5-ALA is metabolized to endogenous fluorescent protoporphyrin IX (PpIX). We examined the accumulation of PpIX in GIST-T1 cells using flow cytometry and immunofluorescent staining. Furthermore, we established GIST-T1 xenograft mouse models and examined PpIX accumulation in the resultant tumors. PpIX accumulated in GIST-T1 cells and was localized mainly to lysosomes. PpIX accumulation was also observed in murine xenograft tumors. Moreover, tumor and normal tissues could be distinctly identified by relative PpIX fluorescence. Thus, our results demonstrated that PDD with 5-ALA has substantial clinical potential for GIST diagnosis. Public Library of Science 2021-04-07 /pmc/articles/PMC8026038/ /pubmed/33826667 http://dx.doi.org/10.1371/journal.pone.0249650 Text en © 2021 Sasaki et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sasaki, Makiko
Tanaka, Mamoru
Ichikawa, Hiroshi
Suzuki, Taketo
Nishie, Hirotada
Ozeki, Keiji
Shimura, Takaya
Kubota, Eiji
Tanida, Satoshi
Kataoka, Hiromi
5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models
title 5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models
title_full 5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models
title_fullStr 5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models
title_full_unstemmed 5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models
title_short 5-aminolaevulinic acid (5-ALA) accumulates in GIST-T1 cells and photodynamic diagnosis using 5-ALA identifies gastrointestinal stromal tumors (GISTs) in xenograft tumor models
title_sort 5-aminolaevulinic acid (5-ala) accumulates in gist-t1 cells and photodynamic diagnosis using 5-ala identifies gastrointestinal stromal tumors (gists) in xenograft tumor models
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026038/
https://www.ncbi.nlm.nih.gov/pubmed/33826667
http://dx.doi.org/10.1371/journal.pone.0249650
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