Genetic engineering in organoids

Three-dimensional organoids have been widely used for developmental and disease modeling. Organoids are derived from both adult and pluripotent stem cells. Various types are available for mimicking almost all major organs and tissues in the mouse and human. While culture protocols for stepwise diffe...

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Detalles Bibliográficos
Autores principales: Teriyapirom, Isaree, Batista-Rocha, Andreia S., Koo, Bon-Kyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026415/
https://www.ncbi.nlm.nih.gov/pubmed/33459801
http://dx.doi.org/10.1007/s00109-020-02029-z
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author Teriyapirom, Isaree
Batista-Rocha, Andreia S.
Koo, Bon-Kyoung
author_facet Teriyapirom, Isaree
Batista-Rocha, Andreia S.
Koo, Bon-Kyoung
author_sort Teriyapirom, Isaree
collection PubMed
description Three-dimensional organoids have been widely used for developmental and disease modeling. Organoids are derived from both adult and pluripotent stem cells. Various types are available for mimicking almost all major organs and tissues in the mouse and human. While culture protocols for stepwise differentiation and long-term expansion are well established, methods for genetic manipulation in organoids still need further standardization. In this review, we summarized different methods for organoid genetics and provide the pros and cons of each method for designing an optimal strategy.
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spelling pubmed-80264152021-04-26 Genetic engineering in organoids Teriyapirom, Isaree Batista-Rocha, Andreia S. Koo, Bon-Kyoung J Mol Med (Berl) Review Three-dimensional organoids have been widely used for developmental and disease modeling. Organoids are derived from both adult and pluripotent stem cells. Various types are available for mimicking almost all major organs and tissues in the mouse and human. While culture protocols for stepwise differentiation and long-term expansion are well established, methods for genetic manipulation in organoids still need further standardization. In this review, we summarized different methods for organoid genetics and provide the pros and cons of each method for designing an optimal strategy. Springer Berlin Heidelberg 2021-01-18 2021 /pmc/articles/PMC8026415/ /pubmed/33459801 http://dx.doi.org/10.1007/s00109-020-02029-z Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Review
Teriyapirom, Isaree
Batista-Rocha, Andreia S.
Koo, Bon-Kyoung
Genetic engineering in organoids
title Genetic engineering in organoids
title_full Genetic engineering in organoids
title_fullStr Genetic engineering in organoids
title_full_unstemmed Genetic engineering in organoids
title_short Genetic engineering in organoids
title_sort genetic engineering in organoids
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026415/
https://www.ncbi.nlm.nih.gov/pubmed/33459801
http://dx.doi.org/10.1007/s00109-020-02029-z
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