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The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference
Animal behaviours are demonstrably governed by sensory stimulation, previous experience and internal states like hunger. With increasing hunger, priorities shift towards foraging and feeding. During foraging, flies are known to employ efficient path integration strategies. However, general long-term...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026880/ https://www.ncbi.nlm.nih.gov/pubmed/33841109 http://dx.doi.org/10.3389/fnbeh.2021.640146 |
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author | Mahishi, Deepthi Triphan, Tilman Hesse, Ricarda Huetteroth, Wolf |
author_facet | Mahishi, Deepthi Triphan, Tilman Hesse, Ricarda Huetteroth, Wolf |
author_sort | Mahishi, Deepthi |
collection | PubMed |
description | Animal behaviours are demonstrably governed by sensory stimulation, previous experience and internal states like hunger. With increasing hunger, priorities shift towards foraging and feeding. During foraging, flies are known to employ efficient path integration strategies. However, general long-term activity patterns for both hungry and satiated flies in conditions of foraging remain to be better understood. Similarly, little is known about how permanent contact chemosensory stimulation affects locomotion. To address these questions, we have developed a novel, simplistic fly activity tracking setup—the Panopticon. Using a 3D-printed Petri dish inset, our assay allows recording of walking behaviour, of several flies in parallel, with all arena surfaces covered by a uniform substrate layer. We tested two constellations of providing food: (i) in single patches and (ii) omnipresent within the substrate layer. Fly tracking is done with FIJI, further assessment, analysis and presentation is done with a custom-built MATLAB analysis framework. We find that starvation history leads to a long-lasting reduction in locomotion, as well as a delayed place preference for food patches which seems to be not driven by immediate hunger motivation. |
format | Online Article Text |
id | pubmed-8026880 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80268802021-04-09 The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference Mahishi, Deepthi Triphan, Tilman Hesse, Ricarda Huetteroth, Wolf Front Behav Neurosci Neuroscience Animal behaviours are demonstrably governed by sensory stimulation, previous experience and internal states like hunger. With increasing hunger, priorities shift towards foraging and feeding. During foraging, flies are known to employ efficient path integration strategies. However, general long-term activity patterns for both hungry and satiated flies in conditions of foraging remain to be better understood. Similarly, little is known about how permanent contact chemosensory stimulation affects locomotion. To address these questions, we have developed a novel, simplistic fly activity tracking setup—the Panopticon. Using a 3D-printed Petri dish inset, our assay allows recording of walking behaviour, of several flies in parallel, with all arena surfaces covered by a uniform substrate layer. We tested two constellations of providing food: (i) in single patches and (ii) omnipresent within the substrate layer. Fly tracking is done with FIJI, further assessment, analysis and presentation is done with a custom-built MATLAB analysis framework. We find that starvation history leads to a long-lasting reduction in locomotion, as well as a delayed place preference for food patches which seems to be not driven by immediate hunger motivation. Frontiers Media S.A. 2021-03-25 /pmc/articles/PMC8026880/ /pubmed/33841109 http://dx.doi.org/10.3389/fnbeh.2021.640146 Text en Copyright © 2021 Mahishi, Triphan, Hesse and Huetteroth. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Mahishi, Deepthi Triphan, Tilman Hesse, Ricarda Huetteroth, Wolf The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference |
title | The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference |
title_full | The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference |
title_fullStr | The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference |
title_full_unstemmed | The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference |
title_short | The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference |
title_sort | panopticon—assessing the effect of starvation on prolonged fly activity and place preference |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026880/ https://www.ncbi.nlm.nih.gov/pubmed/33841109 http://dx.doi.org/10.3389/fnbeh.2021.640146 |
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