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The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference

Animal behaviours are demonstrably governed by sensory stimulation, previous experience and internal states like hunger. With increasing hunger, priorities shift towards foraging and feeding. During foraging, flies are known to employ efficient path integration strategies. However, general long-term...

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Autores principales: Mahishi, Deepthi, Triphan, Tilman, Hesse, Ricarda, Huetteroth, Wolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026880/
https://www.ncbi.nlm.nih.gov/pubmed/33841109
http://dx.doi.org/10.3389/fnbeh.2021.640146
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author Mahishi, Deepthi
Triphan, Tilman
Hesse, Ricarda
Huetteroth, Wolf
author_facet Mahishi, Deepthi
Triphan, Tilman
Hesse, Ricarda
Huetteroth, Wolf
author_sort Mahishi, Deepthi
collection PubMed
description Animal behaviours are demonstrably governed by sensory stimulation, previous experience and internal states like hunger. With increasing hunger, priorities shift towards foraging and feeding. During foraging, flies are known to employ efficient path integration strategies. However, general long-term activity patterns for both hungry and satiated flies in conditions of foraging remain to be better understood. Similarly, little is known about how permanent contact chemosensory stimulation affects locomotion. To address these questions, we have developed a novel, simplistic fly activity tracking setup—the Panopticon. Using a 3D-printed Petri dish inset, our assay allows recording of walking behaviour, of several flies in parallel, with all arena surfaces covered by a uniform substrate layer. We tested two constellations of providing food: (i) in single patches and (ii) omnipresent within the substrate layer. Fly tracking is done with FIJI, further assessment, analysis and presentation is done with a custom-built MATLAB analysis framework. We find that starvation history leads to a long-lasting reduction in locomotion, as well as a delayed place preference for food patches which seems to be not driven by immediate hunger motivation.
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spelling pubmed-80268802021-04-09 The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference Mahishi, Deepthi Triphan, Tilman Hesse, Ricarda Huetteroth, Wolf Front Behav Neurosci Neuroscience Animal behaviours are demonstrably governed by sensory stimulation, previous experience and internal states like hunger. With increasing hunger, priorities shift towards foraging and feeding. During foraging, flies are known to employ efficient path integration strategies. However, general long-term activity patterns for both hungry and satiated flies in conditions of foraging remain to be better understood. Similarly, little is known about how permanent contact chemosensory stimulation affects locomotion. To address these questions, we have developed a novel, simplistic fly activity tracking setup—the Panopticon. Using a 3D-printed Petri dish inset, our assay allows recording of walking behaviour, of several flies in parallel, with all arena surfaces covered by a uniform substrate layer. We tested two constellations of providing food: (i) in single patches and (ii) omnipresent within the substrate layer. Fly tracking is done with FIJI, further assessment, analysis and presentation is done with a custom-built MATLAB analysis framework. We find that starvation history leads to a long-lasting reduction in locomotion, as well as a delayed place preference for food patches which seems to be not driven by immediate hunger motivation. Frontiers Media S.A. 2021-03-25 /pmc/articles/PMC8026880/ /pubmed/33841109 http://dx.doi.org/10.3389/fnbeh.2021.640146 Text en Copyright © 2021 Mahishi, Triphan, Hesse and Huetteroth. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Mahishi, Deepthi
Triphan, Tilman
Hesse, Ricarda
Huetteroth, Wolf
The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference
title The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference
title_full The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference
title_fullStr The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference
title_full_unstemmed The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference
title_short The Panopticon—Assessing the Effect of Starvation on Prolonged Fly Activity and Place Preference
title_sort panopticon—assessing the effect of starvation on prolonged fly activity and place preference
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8026880/
https://www.ncbi.nlm.nih.gov/pubmed/33841109
http://dx.doi.org/10.3389/fnbeh.2021.640146
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