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Arbuscular Mycorrhizal Fungi Alleviate Drought Stress in Trifoliate Orange by Regulating H(+)-ATPase Activity and Gene Expression
A feature of arbuscular mycorrhiza is enhanced drought tolerance of host plants, although it is unclear whether host H(+)-ATPase activity and gene expression are involved in the physiological process. The present study aimed to investigate the effects of an arbuscular mycorrhizal fungus (AMF), Funne...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027329/ https://www.ncbi.nlm.nih.gov/pubmed/33841484 http://dx.doi.org/10.3389/fpls.2021.659694 |
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author | Cheng, Hui-Qian Zou, Ying-Ning Wu, Qiang-Sheng Kuča, Kamil |
author_facet | Cheng, Hui-Qian Zou, Ying-Ning Wu, Qiang-Sheng Kuča, Kamil |
author_sort | Cheng, Hui-Qian |
collection | PubMed |
description | A feature of arbuscular mycorrhiza is enhanced drought tolerance of host plants, although it is unclear whether host H(+)-ATPase activity and gene expression are involved in the physiological process. The present study aimed to investigate the effects of an arbuscular mycorrhizal fungus (AMF), Funneliformis mosseae, on H(+)-ATPase activity, and gene expression of trifoliate orange (Poncirus trifoliata) seedlings subjected to well-watered (WW) and drought stress (DS), together with the changes in leaf gas exchange, root morphology, soil pH value, and ammonium content. Soil drought treatment dramatically increased H(+)-ATPase activity of leaf and root, and AMF inoculation further strengthened the increased effect. A plasma membrane (PM) H(+)-ATPase gene of trifoliate orange, PtAHA2 (MW239123), was cloned. The PtAHA2 expression was induced by mycorrhization in leaves and roots and also up-regulated by drought treatment in leaves of AMF-inoculated seedlings and in roots of AMF- and non-AMF-inoculated seedlings. And, the induced expression of PtAHA2 under mycorrhization was more prominent under DS than under WW. Mycorrhizal plants also showed greater photosynthetic rate, stomatal conductance, intercellular CO(2) concentration, and transpiration rate and better root volume and diameter than non-mycorrhizal plants under DS. AMF inoculation significantly increased leaf and root ammonium content, especially under DS, whereas it dramatically reduced soil pH value. In addition, H(+)-ATPase activity was significantly positively correlated with ammonium contents in leaves and roots, and root H(+)-ATPase activity was significantly negatively correlated with soil pH value. Our results concluded that AMF stimulated H(+)-ATPase activity and PtAHA2 gene expression in response to DS, which resulted in great nutrient (e.g., ammonium) uptake and root growth, as well as low soil pH microenvironment. |
format | Online Article Text |
id | pubmed-8027329 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-80273292021-04-09 Arbuscular Mycorrhizal Fungi Alleviate Drought Stress in Trifoliate Orange by Regulating H(+)-ATPase Activity and Gene Expression Cheng, Hui-Qian Zou, Ying-Ning Wu, Qiang-Sheng Kuča, Kamil Front Plant Sci Plant Science A feature of arbuscular mycorrhiza is enhanced drought tolerance of host plants, although it is unclear whether host H(+)-ATPase activity and gene expression are involved in the physiological process. The present study aimed to investigate the effects of an arbuscular mycorrhizal fungus (AMF), Funneliformis mosseae, on H(+)-ATPase activity, and gene expression of trifoliate orange (Poncirus trifoliata) seedlings subjected to well-watered (WW) and drought stress (DS), together with the changes in leaf gas exchange, root morphology, soil pH value, and ammonium content. Soil drought treatment dramatically increased H(+)-ATPase activity of leaf and root, and AMF inoculation further strengthened the increased effect. A plasma membrane (PM) H(+)-ATPase gene of trifoliate orange, PtAHA2 (MW239123), was cloned. The PtAHA2 expression was induced by mycorrhization in leaves and roots and also up-regulated by drought treatment in leaves of AMF-inoculated seedlings and in roots of AMF- and non-AMF-inoculated seedlings. And, the induced expression of PtAHA2 under mycorrhization was more prominent under DS than under WW. Mycorrhizal plants also showed greater photosynthetic rate, stomatal conductance, intercellular CO(2) concentration, and transpiration rate and better root volume and diameter than non-mycorrhizal plants under DS. AMF inoculation significantly increased leaf and root ammonium content, especially under DS, whereas it dramatically reduced soil pH value. In addition, H(+)-ATPase activity was significantly positively correlated with ammonium contents in leaves and roots, and root H(+)-ATPase activity was significantly negatively correlated with soil pH value. Our results concluded that AMF stimulated H(+)-ATPase activity and PtAHA2 gene expression in response to DS, which resulted in great nutrient (e.g., ammonium) uptake and root growth, as well as low soil pH microenvironment. Frontiers Media S.A. 2021-03-25 /pmc/articles/PMC8027329/ /pubmed/33841484 http://dx.doi.org/10.3389/fpls.2021.659694 Text en Copyright © 2021 Cheng, Zou, Wu and Kuča. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Cheng, Hui-Qian Zou, Ying-Ning Wu, Qiang-Sheng Kuča, Kamil Arbuscular Mycorrhizal Fungi Alleviate Drought Stress in Trifoliate Orange by Regulating H(+)-ATPase Activity and Gene Expression |
title | Arbuscular Mycorrhizal Fungi Alleviate Drought Stress in Trifoliate Orange by Regulating H(+)-ATPase Activity and Gene Expression |
title_full | Arbuscular Mycorrhizal Fungi Alleviate Drought Stress in Trifoliate Orange by Regulating H(+)-ATPase Activity and Gene Expression |
title_fullStr | Arbuscular Mycorrhizal Fungi Alleviate Drought Stress in Trifoliate Orange by Regulating H(+)-ATPase Activity and Gene Expression |
title_full_unstemmed | Arbuscular Mycorrhizal Fungi Alleviate Drought Stress in Trifoliate Orange by Regulating H(+)-ATPase Activity and Gene Expression |
title_short | Arbuscular Mycorrhizal Fungi Alleviate Drought Stress in Trifoliate Orange by Regulating H(+)-ATPase Activity and Gene Expression |
title_sort | arbuscular mycorrhizal fungi alleviate drought stress in trifoliate orange by regulating h(+)-atpase activity and gene expression |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027329/ https://www.ncbi.nlm.nih.gov/pubmed/33841484 http://dx.doi.org/10.3389/fpls.2021.659694 |
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