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Transcriptomic Changes Induced by Deletion of Transcriptional Regulator GCR2 on Pentose Sugar Metabolism in Saccharomyces cerevisiae

Being a microbial host for lignocellulosic biofuel production, Saccharomyces cerevisiae needs to be engineered to express a heterologous xylose pathway; however, it has been challenging to optimize the engineered strain for efficient and rapid fermentation of xylose. Deletion of PHO13 (Δpho13) has b...

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Autores principales: Shin, Minhye, Park, Heeyoung, Kim, Sooah, Oh, Eun Joong, Jeong, Deokyeol, Florencia, Clarissa, Kim, Kyoung Heon, Jin, Yong-Su, Kim, Soo Rin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027353/
https://www.ncbi.nlm.nih.gov/pubmed/33842449
http://dx.doi.org/10.3389/fbioe.2021.654177
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author Shin, Minhye
Park, Heeyoung
Kim, Sooah
Oh, Eun Joong
Jeong, Deokyeol
Florencia, Clarissa
Kim, Kyoung Heon
Jin, Yong-Su
Kim, Soo Rin
author_facet Shin, Minhye
Park, Heeyoung
Kim, Sooah
Oh, Eun Joong
Jeong, Deokyeol
Florencia, Clarissa
Kim, Kyoung Heon
Jin, Yong-Su
Kim, Soo Rin
author_sort Shin, Minhye
collection PubMed
description Being a microbial host for lignocellulosic biofuel production, Saccharomyces cerevisiae needs to be engineered to express a heterologous xylose pathway; however, it has been challenging to optimize the engineered strain for efficient and rapid fermentation of xylose. Deletion of PHO13 (Δpho13) has been reported to be a crucial genetic perturbation in improving xylose fermentation. A confirmed mechanism of the Δpho13 effect on xylose fermentation is that the Δpho13 transcriptionally activates the genes in the non-oxidative pentose phosphate pathway (PPP). In the current study, we found a couple of engineered strains, of which phenotypes were not affected by Δpho13 (Δpho13-negative), among many others we examined. Genome resequencing of the Δpho13-negative strains revealed that a loss-of-function mutation in GCR2 was responsible for the phenotype. Gcr2 is a global transcriptional factor involved in glucose metabolism. The results of RNA-seq confirmed that the deletion of GCR2 (Δgcr2) led to the upregulation of PPP genes as well as downregulation of glycolytic genes, and changes were more significant under xylose conditions than those under glucose conditions. Although there was no synergistic effect between Δpho13 and Δgcr2 in improving xylose fermentation, these results suggested that GCR2 is a novel knockout target in improving lignocellulosic ethanol production.
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spelling pubmed-80273532021-04-09 Transcriptomic Changes Induced by Deletion of Transcriptional Regulator GCR2 on Pentose Sugar Metabolism in Saccharomyces cerevisiae Shin, Minhye Park, Heeyoung Kim, Sooah Oh, Eun Joong Jeong, Deokyeol Florencia, Clarissa Kim, Kyoung Heon Jin, Yong-Su Kim, Soo Rin Front Bioeng Biotechnol Bioengineering and Biotechnology Being a microbial host for lignocellulosic biofuel production, Saccharomyces cerevisiae needs to be engineered to express a heterologous xylose pathway; however, it has been challenging to optimize the engineered strain for efficient and rapid fermentation of xylose. Deletion of PHO13 (Δpho13) has been reported to be a crucial genetic perturbation in improving xylose fermentation. A confirmed mechanism of the Δpho13 effect on xylose fermentation is that the Δpho13 transcriptionally activates the genes in the non-oxidative pentose phosphate pathway (PPP). In the current study, we found a couple of engineered strains, of which phenotypes were not affected by Δpho13 (Δpho13-negative), among many others we examined. Genome resequencing of the Δpho13-negative strains revealed that a loss-of-function mutation in GCR2 was responsible for the phenotype. Gcr2 is a global transcriptional factor involved in glucose metabolism. The results of RNA-seq confirmed that the deletion of GCR2 (Δgcr2) led to the upregulation of PPP genes as well as downregulation of glycolytic genes, and changes were more significant under xylose conditions than those under glucose conditions. Although there was no synergistic effect between Δpho13 and Δgcr2 in improving xylose fermentation, these results suggested that GCR2 is a novel knockout target in improving lignocellulosic ethanol production. Frontiers Media S.A. 2021-03-25 /pmc/articles/PMC8027353/ /pubmed/33842449 http://dx.doi.org/10.3389/fbioe.2021.654177 Text en Copyright © 2021 Shin, Park, Kim, Oh, Jeong, Florencia, Kim, Jin and Kim. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Shin, Minhye
Park, Heeyoung
Kim, Sooah
Oh, Eun Joong
Jeong, Deokyeol
Florencia, Clarissa
Kim, Kyoung Heon
Jin, Yong-Su
Kim, Soo Rin
Transcriptomic Changes Induced by Deletion of Transcriptional Regulator GCR2 on Pentose Sugar Metabolism in Saccharomyces cerevisiae
title Transcriptomic Changes Induced by Deletion of Transcriptional Regulator GCR2 on Pentose Sugar Metabolism in Saccharomyces cerevisiae
title_full Transcriptomic Changes Induced by Deletion of Transcriptional Regulator GCR2 on Pentose Sugar Metabolism in Saccharomyces cerevisiae
title_fullStr Transcriptomic Changes Induced by Deletion of Transcriptional Regulator GCR2 on Pentose Sugar Metabolism in Saccharomyces cerevisiae
title_full_unstemmed Transcriptomic Changes Induced by Deletion of Transcriptional Regulator GCR2 on Pentose Sugar Metabolism in Saccharomyces cerevisiae
title_short Transcriptomic Changes Induced by Deletion of Transcriptional Regulator GCR2 on Pentose Sugar Metabolism in Saccharomyces cerevisiae
title_sort transcriptomic changes induced by deletion of transcriptional regulator gcr2 on pentose sugar metabolism in saccharomyces cerevisiae
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027353/
https://www.ncbi.nlm.nih.gov/pubmed/33842449
http://dx.doi.org/10.3389/fbioe.2021.654177
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