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Direct interactions with commensal streptococci modify intercellular communication behaviors of Streptococcus mutans

The formation of dental caries is a complex process that ultimately leads to damage of the tooth enamel from acids produced by microbes in attached biofilms. The bacterial interactions occurring within these biofilms between cariogenic bacteria, such as the mutans streptococci, and health-associated...

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Autores principales: Kaspar, Justin R., Lee, Kyulim, Richard, Brook, Walker, Alejandro R., Burne, Robert A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027600/
https://www.ncbi.nlm.nih.gov/pubmed/32999420
http://dx.doi.org/10.1038/s41396-020-00789-7
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author Kaspar, Justin R.
Lee, Kyulim
Richard, Brook
Walker, Alejandro R.
Burne, Robert A.
author_facet Kaspar, Justin R.
Lee, Kyulim
Richard, Brook
Walker, Alejandro R.
Burne, Robert A.
author_sort Kaspar, Justin R.
collection PubMed
description The formation of dental caries is a complex process that ultimately leads to damage of the tooth enamel from acids produced by microbes in attached biofilms. The bacterial interactions occurring within these biofilms between cariogenic bacteria, such as the mutans streptococci, and health-associated commensal streptococci, are thought to be critical determinants of health and disease. To better understand these interactions, a Streptococcus mutans reporter strain that actively monitors cell–cell communication via peptide signaling was cocultured with different commensal streptococci. Signaling by S. mutans, normally highly active in monoculture, was completely inhibited by several species of commensals, but only when the bacteria were in direct contact with S. mutans. We identified a novel gene expression pattern that occurred in S. mutans when cultured directly with these commensals. Finally, mutant derivatives of commensals lacking previously shown antagonistic gene products displayed wild-type levels of signal inhibition in cocultures. Collectively, these results reveal a novel pathway(s) in multiple health-associated commensal streptococci that blocks peptide signaling and induces a common contact-dependent pattern of differential gene expression in S. mutans. Understanding the molecular basis for this inhibition will assist in the rational design of new risk assessments, diagnostics, and treatments for the most pervasive oral infectious diseases.
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spelling pubmed-80276002021-04-21 Direct interactions with commensal streptococci modify intercellular communication behaviors of Streptococcus mutans Kaspar, Justin R. Lee, Kyulim Richard, Brook Walker, Alejandro R. Burne, Robert A. ISME J Article The formation of dental caries is a complex process that ultimately leads to damage of the tooth enamel from acids produced by microbes in attached biofilms. The bacterial interactions occurring within these biofilms between cariogenic bacteria, such as the mutans streptococci, and health-associated commensal streptococci, are thought to be critical determinants of health and disease. To better understand these interactions, a Streptococcus mutans reporter strain that actively monitors cell–cell communication via peptide signaling was cocultured with different commensal streptococci. Signaling by S. mutans, normally highly active in monoculture, was completely inhibited by several species of commensals, but only when the bacteria were in direct contact with S. mutans. We identified a novel gene expression pattern that occurred in S. mutans when cultured directly with these commensals. Finally, mutant derivatives of commensals lacking previously shown antagonistic gene products displayed wild-type levels of signal inhibition in cocultures. Collectively, these results reveal a novel pathway(s) in multiple health-associated commensal streptococci that blocks peptide signaling and induces a common contact-dependent pattern of differential gene expression in S. mutans. Understanding the molecular basis for this inhibition will assist in the rational design of new risk assessments, diagnostics, and treatments for the most pervasive oral infectious diseases. Nature Publishing Group UK 2020-09-30 2021-02 /pmc/articles/PMC8027600/ /pubmed/32999420 http://dx.doi.org/10.1038/s41396-020-00789-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kaspar, Justin R.
Lee, Kyulim
Richard, Brook
Walker, Alejandro R.
Burne, Robert A.
Direct interactions with commensal streptococci modify intercellular communication behaviors of Streptococcus mutans
title Direct interactions with commensal streptococci modify intercellular communication behaviors of Streptococcus mutans
title_full Direct interactions with commensal streptococci modify intercellular communication behaviors of Streptococcus mutans
title_fullStr Direct interactions with commensal streptococci modify intercellular communication behaviors of Streptococcus mutans
title_full_unstemmed Direct interactions with commensal streptococci modify intercellular communication behaviors of Streptococcus mutans
title_short Direct interactions with commensal streptococci modify intercellular communication behaviors of Streptococcus mutans
title_sort direct interactions with commensal streptococci modify intercellular communication behaviors of streptococcus mutans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027600/
https://www.ncbi.nlm.nih.gov/pubmed/32999420
http://dx.doi.org/10.1038/s41396-020-00789-7
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