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Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil
Pongamia pinnata (L.) seed oil is effective for its insecticidal and larvicidal activities. However, its low aqueous solubility, high photosensitivity, and high volatility restrict its application for the control of agricultural pests. Encapsulation can be an effective technique to overcome such hin...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027697/ https://www.ncbi.nlm.nih.gov/pubmed/33855235 http://dx.doi.org/10.1016/j.heliyon.2021.e06557 |
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author | Purkait, Aloke Mukherjee, Ayan Hazra, Dipak Kumar Roy, Kusal Biswas, Pabitra Kumar Kole, Ramen Kumar |
author_facet | Purkait, Aloke Mukherjee, Ayan Hazra, Dipak Kumar Roy, Kusal Biswas, Pabitra Kumar Kole, Ramen Kumar |
author_sort | Purkait, Aloke |
collection | PubMed |
description | Pongamia pinnata (L.) seed oil is effective for its insecticidal and larvicidal activities. However, its low aqueous solubility, high photosensitivity, and high volatility restrict its application for the control of agricultural pests. Encapsulation can be an effective technique to overcome such hindrances. Therefore, P. pinnata oil (PO) was extracted from its seeds and analyzed for karanjin content (3.18%) by GC-MS/MS as the marker compound. Micro-encapsulation (MC) of PO was prepared by interfacial polymerization between isocyanates and polyamine and tested for insecticidal and larvicidal activities. Bioassay of the developed formulations was tested in-vitro against 2(nd) instar larvae of Bombyx mori (Bivoltine hybrid) and in-vivo insecticidal bio-efficacy was tested against aubergine aphid (Aphis gossypii G.) and whitefly (Bemisia tabaci G.). Various properties of micro-capsules viz., stability, size, oil content and release kinetics were examined. Average diameter of capsules (1 μm) with Zeta potential (-16 mV) was indicated by the Dynamic Light Scattering (DLS) instrument. Existence of PO in the microcapsules was confirmed by an optical microscopic study. Spectroscopic analysis revealed 87.4% of PO was encapsulated in polyurea shell. The shelf-life (T(10)), half-life (T(50)), and expiry-life (T(90)) of polyurea coated capsules were 11.4, 75.3 and 250.0 h, respectively. Polyurea coated PO capsule formulation showed evidence of in-vitro toxicity against 2(nd) instar larvae of B. mori (LC(50)= 1.1%; LC(90) = 5.9%). The PO formulation also exhibited 67.0–71.8% and 62.4–74.8% control of aphid and whitefly population in aubergine at 4.0% dose following 7–14 days after application. The study unveiled its significance in developing controlled release herbal formulations of P. pinnata as an alternative to harmful conventional synthetic insecticides for crop protection. |
format | Online Article Text |
id | pubmed-8027697 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-80276972021-04-13 Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil Purkait, Aloke Mukherjee, Ayan Hazra, Dipak Kumar Roy, Kusal Biswas, Pabitra Kumar Kole, Ramen Kumar Heliyon Research Article Pongamia pinnata (L.) seed oil is effective for its insecticidal and larvicidal activities. However, its low aqueous solubility, high photosensitivity, and high volatility restrict its application for the control of agricultural pests. Encapsulation can be an effective technique to overcome such hindrances. Therefore, P. pinnata oil (PO) was extracted from its seeds and analyzed for karanjin content (3.18%) by GC-MS/MS as the marker compound. Micro-encapsulation (MC) of PO was prepared by interfacial polymerization between isocyanates and polyamine and tested for insecticidal and larvicidal activities. Bioassay of the developed formulations was tested in-vitro against 2(nd) instar larvae of Bombyx mori (Bivoltine hybrid) and in-vivo insecticidal bio-efficacy was tested against aubergine aphid (Aphis gossypii G.) and whitefly (Bemisia tabaci G.). Various properties of micro-capsules viz., stability, size, oil content and release kinetics were examined. Average diameter of capsules (1 μm) with Zeta potential (-16 mV) was indicated by the Dynamic Light Scattering (DLS) instrument. Existence of PO in the microcapsules was confirmed by an optical microscopic study. Spectroscopic analysis revealed 87.4% of PO was encapsulated in polyurea shell. The shelf-life (T(10)), half-life (T(50)), and expiry-life (T(90)) of polyurea coated capsules were 11.4, 75.3 and 250.0 h, respectively. Polyurea coated PO capsule formulation showed evidence of in-vitro toxicity against 2(nd) instar larvae of B. mori (LC(50)= 1.1%; LC(90) = 5.9%). The PO formulation also exhibited 67.0–71.8% and 62.4–74.8% control of aphid and whitefly population in aubergine at 4.0% dose following 7–14 days after application. The study unveiled its significance in developing controlled release herbal formulations of P. pinnata as an alternative to harmful conventional synthetic insecticides for crop protection. Elsevier 2021-03-24 /pmc/articles/PMC8027697/ /pubmed/33855235 http://dx.doi.org/10.1016/j.heliyon.2021.e06557 Text en © 2021 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Article Purkait, Aloke Mukherjee, Ayan Hazra, Dipak Kumar Roy, Kusal Biswas, Pabitra Kumar Kole, Ramen Kumar Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil |
title | Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil |
title_full | Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil |
title_fullStr | Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil |
title_full_unstemmed | Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil |
title_short | Encapsulation, release and insecticidal activity of Pongamia pinnata (L.) seed oil |
title_sort | encapsulation, release and insecticidal activity of pongamia pinnata (l.) seed oil |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8027697/ https://www.ncbi.nlm.nih.gov/pubmed/33855235 http://dx.doi.org/10.1016/j.heliyon.2021.e06557 |
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