Cargando…

Nanoindentation for Monitoring the Time-Variant Mechanical Strength of Drug-Loaded Collagen Hydrogel Regulated by Hydroxyapatite Nanoparticles

[Image: see text] Hydroxyapatite nanoparticle-complexed collagen (HAP/Col) hydrogels have been widely used in biomedical applications as a scaffold for controlled drug release (DR). The time-variant mechanical properties (Young’s modulus, E) of HAP/Col hydrogels are highly relevant to the precise an...

Descripción completa

Detalles Bibliográficos
Autores principales: Jung, Hyo Gi, Lee, Dongtak, Lee, Sang Won, Kim, Insu, Kim, Yonghwan, Jang, Jae Won, Lee, Jeong Hoon, Lee, Gyudo, Yoon, Dae Sung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028154/
https://www.ncbi.nlm.nih.gov/pubmed/33842796
http://dx.doi.org/10.1021/acsomega.1c00824
_version_ 1783675933446635520
author Jung, Hyo Gi
Lee, Dongtak
Lee, Sang Won
Kim, Insu
Kim, Yonghwan
Jang, Jae Won
Lee, Jeong Hoon
Lee, Gyudo
Yoon, Dae Sung
author_facet Jung, Hyo Gi
Lee, Dongtak
Lee, Sang Won
Kim, Insu
Kim, Yonghwan
Jang, Jae Won
Lee, Jeong Hoon
Lee, Gyudo
Yoon, Dae Sung
author_sort Jung, Hyo Gi
collection PubMed
description [Image: see text] Hydroxyapatite nanoparticle-complexed collagen (HAP/Col) hydrogels have been widely used in biomedical applications as a scaffold for controlled drug release (DR). The time-variant mechanical properties (Young’s modulus, E) of HAP/Col hydrogels are highly relevant to the precise and efficient control of DR. However, the correlation between the DR and the E of hydrogels remains unclear because of the lack of a nondestructive and continuous measuring system. To reveal the correlations, herein, we investigate the time-variant behavior of E for HAP/Col hydrogels during 28 days using the atomic force microscopy (AFM) nanoindentation technique. The initial E of hydrogels was controlled from 200 to 9000 Pa by the addition of HAPs. Subsequently, we analyzed the relationship between the DR of the hydrogels and the changes in their mechanical properties (ΔE) during hydrogel degradation. Interestingly, the higher the initial E value of HAP/Col hydrogels is, the higher is the rate of hydrogel degradation over time. However, the DR of hydrogels with higher initial E appeared to be significantly delayed by up to 40% at a maximum. The results indicate that adding an appropriate amount of HAPs into hydrogels plays a crucial role in determining the initial E and their degradation rate, which can contribute to the properties that prolong DR. Our findings may provide insights into designing hydrogels for biomedical applications such as bone regeneration and drug-delivery systems.
format Online
Article
Text
id pubmed-8028154
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-80281542021-04-09 Nanoindentation for Monitoring the Time-Variant Mechanical Strength of Drug-Loaded Collagen Hydrogel Regulated by Hydroxyapatite Nanoparticles Jung, Hyo Gi Lee, Dongtak Lee, Sang Won Kim, Insu Kim, Yonghwan Jang, Jae Won Lee, Jeong Hoon Lee, Gyudo Yoon, Dae Sung ACS Omega [Image: see text] Hydroxyapatite nanoparticle-complexed collagen (HAP/Col) hydrogels have been widely used in biomedical applications as a scaffold for controlled drug release (DR). The time-variant mechanical properties (Young’s modulus, E) of HAP/Col hydrogels are highly relevant to the precise and efficient control of DR. However, the correlation between the DR and the E of hydrogels remains unclear because of the lack of a nondestructive and continuous measuring system. To reveal the correlations, herein, we investigate the time-variant behavior of E for HAP/Col hydrogels during 28 days using the atomic force microscopy (AFM) nanoindentation technique. The initial E of hydrogels was controlled from 200 to 9000 Pa by the addition of HAPs. Subsequently, we analyzed the relationship between the DR of the hydrogels and the changes in their mechanical properties (ΔE) during hydrogel degradation. Interestingly, the higher the initial E value of HAP/Col hydrogels is, the higher is the rate of hydrogel degradation over time. However, the DR of hydrogels with higher initial E appeared to be significantly delayed by up to 40% at a maximum. The results indicate that adding an appropriate amount of HAPs into hydrogels plays a crucial role in determining the initial E and their degradation rate, which can contribute to the properties that prolong DR. Our findings may provide insights into designing hydrogels for biomedical applications such as bone regeneration and drug-delivery systems. American Chemical Society 2021-03-23 /pmc/articles/PMC8028154/ /pubmed/33842796 http://dx.doi.org/10.1021/acsomega.1c00824 Text en © 2021 The Authors. Published by American Chemical Society Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Jung, Hyo Gi
Lee, Dongtak
Lee, Sang Won
Kim, Insu
Kim, Yonghwan
Jang, Jae Won
Lee, Jeong Hoon
Lee, Gyudo
Yoon, Dae Sung
Nanoindentation for Monitoring the Time-Variant Mechanical Strength of Drug-Loaded Collagen Hydrogel Regulated by Hydroxyapatite Nanoparticles
title Nanoindentation for Monitoring the Time-Variant Mechanical Strength of Drug-Loaded Collagen Hydrogel Regulated by Hydroxyapatite Nanoparticles
title_full Nanoindentation for Monitoring the Time-Variant Mechanical Strength of Drug-Loaded Collagen Hydrogel Regulated by Hydroxyapatite Nanoparticles
title_fullStr Nanoindentation for Monitoring the Time-Variant Mechanical Strength of Drug-Loaded Collagen Hydrogel Regulated by Hydroxyapatite Nanoparticles
title_full_unstemmed Nanoindentation for Monitoring the Time-Variant Mechanical Strength of Drug-Loaded Collagen Hydrogel Regulated by Hydroxyapatite Nanoparticles
title_short Nanoindentation for Monitoring the Time-Variant Mechanical Strength of Drug-Loaded Collagen Hydrogel Regulated by Hydroxyapatite Nanoparticles
title_sort nanoindentation for monitoring the time-variant mechanical strength of drug-loaded collagen hydrogel regulated by hydroxyapatite nanoparticles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8028154/
https://www.ncbi.nlm.nih.gov/pubmed/33842796
http://dx.doi.org/10.1021/acsomega.1c00824
work_keys_str_mv AT junghyogi nanoindentationformonitoringthetimevariantmechanicalstrengthofdrugloadedcollagenhydrogelregulatedbyhydroxyapatitenanoparticles
AT leedongtak nanoindentationformonitoringthetimevariantmechanicalstrengthofdrugloadedcollagenhydrogelregulatedbyhydroxyapatitenanoparticles
AT leesangwon nanoindentationformonitoringthetimevariantmechanicalstrengthofdrugloadedcollagenhydrogelregulatedbyhydroxyapatitenanoparticles
AT kiminsu nanoindentationformonitoringthetimevariantmechanicalstrengthofdrugloadedcollagenhydrogelregulatedbyhydroxyapatitenanoparticles
AT kimyonghwan nanoindentationformonitoringthetimevariantmechanicalstrengthofdrugloadedcollagenhydrogelregulatedbyhydroxyapatitenanoparticles
AT jangjaewon nanoindentationformonitoringthetimevariantmechanicalstrengthofdrugloadedcollagenhydrogelregulatedbyhydroxyapatitenanoparticles
AT leejeonghoon nanoindentationformonitoringthetimevariantmechanicalstrengthofdrugloadedcollagenhydrogelregulatedbyhydroxyapatitenanoparticles
AT leegyudo nanoindentationformonitoringthetimevariantmechanicalstrengthofdrugloadedcollagenhydrogelregulatedbyhydroxyapatitenanoparticles
AT yoondaesung nanoindentationformonitoringthetimevariantmechanicalstrengthofdrugloadedcollagenhydrogelregulatedbyhydroxyapatitenanoparticles