Cloning of human ABCB11 gene in E. coli required the removal of an intragenic Pribnow-Schaller Box before it’s Insertion into genomic safe harbor AAVS1 site using CRISPR–Cas9

Background: Genomic safe harbors are sites in the genome which are safe for gene insertion such that the inserted gene will function properly, and the disruption of the genomic location doesn’t cause any foreseeable risk to the host. The AAVS1 site is the genetic location which is disrupted upon int...

Descripción completa

Detalles Bibliográficos
Autores principales: Vats, Nisha, Sanal, Madhusudana Girija, Venugopal, Senthil Kumar, Taneja, Pankaj, Sarin, Shiv Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2020
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8030117/
https://www.ncbi.nlm.nih.gov/pubmed/33868646
http://dx.doi.org/10.12688/f1000research.26659.1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8030117/
https://www.ncbi.nlm.nih.gov/pubmed/33868646
http://dx.doi.org/10.12688/f1000research.26659.1

Ejemplares similares