Cargando…
Comparison of a one-step real-time RT-PCR and a nested real-time RT-PCR for a genogroup II norovirus reveals differences in sensitivity depending upon assay design and visualization
Human norovirus (NoV) is the leading cause of acute viral gastroenteritis and a major source of foodborne illness. Detection of NoV in food and environmental samples is typically performed using molecular techniques, including real-time reverse transcription polymerase chain reaction (RT-PCR) and le...
Autores principales: | Manuel, Clyde S., Suther, Cassandra, Moore, Matthew D., Jaykus, Lee-Ann |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8031402/ https://www.ncbi.nlm.nih.gov/pubmed/33831019 http://dx.doi.org/10.1371/journal.pone.0248581 |
Ejemplares similares
-
Detection of Norovirus genogroup I and II by multiplex real-time RT- PCR using a 3'-minor groove binder-DNA probe
por: Hoehne, Marina, et al.
Publicado: (2006) -
A Real-Time RT-PCR Assay for Genotyping of Rotavirus
por: Mousavi-Nasab, Seyed Dawood, et al.
Publicado: (2020) -
A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus
por: Hanaki, Ken-Ichi, et al.
Publicado: (2014) -
Comparative Evaluation of Standard RT-PCR Assays and Commercial Real-Time RT-PCR Kits for Detection of Lassa Virus
por: Luo, Xue-Lian, et al.
Publicado: (2023) -
Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study
por: Jalal, Sehrish, et al.
Publicado: (2021)