Cargando…

Microbial community compositions in breast implant biofilms associated with contracted capsules

Subclinical bacterial infections (biofilms) are strongly implicated in breast augmentation failure due to capsular contracture, and while these infections are generally ascribed to common skin commensals, this remains largely unsubstantiated through robust cultivation independent analyses. To determ...

Descripción completa

Detalles Bibliográficos
Autores principales: Crowe, Sean A., Simister, Rachel L., Spence, Jenifer S., Kenward, Paul A., Van Slyke, Aaron C., Lennox, Peter, Carr, Nick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8031459/
https://www.ncbi.nlm.nih.gov/pubmed/33831065
http://dx.doi.org/10.1371/journal.pone.0249261
_version_ 1783676170068295680
author Crowe, Sean A.
Simister, Rachel L.
Spence, Jenifer S.
Kenward, Paul A.
Van Slyke, Aaron C.
Lennox, Peter
Carr, Nick
author_facet Crowe, Sean A.
Simister, Rachel L.
Spence, Jenifer S.
Kenward, Paul A.
Van Slyke, Aaron C.
Lennox, Peter
Carr, Nick
author_sort Crowe, Sean A.
collection PubMed
description Subclinical bacterial infections (biofilms) are strongly implicated in breast augmentation failure due to capsular contracture, and while these infections are generally ascribed to common skin commensals, this remains largely unsubstantiated through robust cultivation independent analyses. To determine capsule biofilm microbial community compositions, we employed amplicon sequencing of the 16S rRNA gene using DNA extracted from breast implant capsule samples. These cultivation independent analyses revealed that capsule associated biofilms are more diverse than canonical single-species infections, but have relatively low diversity (~ <100 species) compared to many host-associated microbial communities. In addition to taxa commonly associated with capsular contracture, the biofilms analyzed comprised a number of taxa that escaped detection in cultivation-dependent work. We have also isolated several key taxa identified through the culture-independent analyses. Together our analyses reveal that capsule biofilms are more diverse than cultivation studies suggest and can be heterogeneous within an individual capsule, between breasts of the same patient, across similar implant types, and over a range in severity of contracture. The complex nature of these communities requires further study across a broader suite of patients in addition to higher resolution analyses including metagenomics to better assess the fundamental role of microorganisms in capsular contracture.
format Online
Article
Text
id pubmed-8031459
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-80314592021-04-14 Microbial community compositions in breast implant biofilms associated with contracted capsules Crowe, Sean A. Simister, Rachel L. Spence, Jenifer S. Kenward, Paul A. Van Slyke, Aaron C. Lennox, Peter Carr, Nick PLoS One Research Article Subclinical bacterial infections (biofilms) are strongly implicated in breast augmentation failure due to capsular contracture, and while these infections are generally ascribed to common skin commensals, this remains largely unsubstantiated through robust cultivation independent analyses. To determine capsule biofilm microbial community compositions, we employed amplicon sequencing of the 16S rRNA gene using DNA extracted from breast implant capsule samples. These cultivation independent analyses revealed that capsule associated biofilms are more diverse than canonical single-species infections, but have relatively low diversity (~ <100 species) compared to many host-associated microbial communities. In addition to taxa commonly associated with capsular contracture, the biofilms analyzed comprised a number of taxa that escaped detection in cultivation-dependent work. We have also isolated several key taxa identified through the culture-independent analyses. Together our analyses reveal that capsule biofilms are more diverse than cultivation studies suggest and can be heterogeneous within an individual capsule, between breasts of the same patient, across similar implant types, and over a range in severity of contracture. The complex nature of these communities requires further study across a broader suite of patients in addition to higher resolution analyses including metagenomics to better assess the fundamental role of microorganisms in capsular contracture. Public Library of Science 2021-04-08 /pmc/articles/PMC8031459/ /pubmed/33831065 http://dx.doi.org/10.1371/journal.pone.0249261 Text en © 2021 Crowe et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Crowe, Sean A.
Simister, Rachel L.
Spence, Jenifer S.
Kenward, Paul A.
Van Slyke, Aaron C.
Lennox, Peter
Carr, Nick
Microbial community compositions in breast implant biofilms associated with contracted capsules
title Microbial community compositions in breast implant biofilms associated with contracted capsules
title_full Microbial community compositions in breast implant biofilms associated with contracted capsules
title_fullStr Microbial community compositions in breast implant biofilms associated with contracted capsules
title_full_unstemmed Microbial community compositions in breast implant biofilms associated with contracted capsules
title_short Microbial community compositions in breast implant biofilms associated with contracted capsules
title_sort microbial community compositions in breast implant biofilms associated with contracted capsules
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8031459/
https://www.ncbi.nlm.nih.gov/pubmed/33831065
http://dx.doi.org/10.1371/journal.pone.0249261
work_keys_str_mv AT croweseana microbialcommunitycompositionsinbreastimplantbiofilmsassociatedwithcontractedcapsules
AT simisterrachell microbialcommunitycompositionsinbreastimplantbiofilmsassociatedwithcontractedcapsules
AT spencejenifers microbialcommunitycompositionsinbreastimplantbiofilmsassociatedwithcontractedcapsules
AT kenwardpaula microbialcommunitycompositionsinbreastimplantbiofilmsassociatedwithcontractedcapsules
AT vanslykeaaronc microbialcommunitycompositionsinbreastimplantbiofilmsassociatedwithcontractedcapsules
AT lennoxpeter microbialcommunitycompositionsinbreastimplantbiofilmsassociatedwithcontractedcapsules
AT carrnick microbialcommunitycompositionsinbreastimplantbiofilmsassociatedwithcontractedcapsules