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Microbial community compositions in breast implant biofilms associated with contracted capsules
Subclinical bacterial infections (biofilms) are strongly implicated in breast augmentation failure due to capsular contracture, and while these infections are generally ascribed to common skin commensals, this remains largely unsubstantiated through robust cultivation independent analyses. To determ...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8031459/ https://www.ncbi.nlm.nih.gov/pubmed/33831065 http://dx.doi.org/10.1371/journal.pone.0249261 |
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author | Crowe, Sean A. Simister, Rachel L. Spence, Jenifer S. Kenward, Paul A. Van Slyke, Aaron C. Lennox, Peter Carr, Nick |
author_facet | Crowe, Sean A. Simister, Rachel L. Spence, Jenifer S. Kenward, Paul A. Van Slyke, Aaron C. Lennox, Peter Carr, Nick |
author_sort | Crowe, Sean A. |
collection | PubMed |
description | Subclinical bacterial infections (biofilms) are strongly implicated in breast augmentation failure due to capsular contracture, and while these infections are generally ascribed to common skin commensals, this remains largely unsubstantiated through robust cultivation independent analyses. To determine capsule biofilm microbial community compositions, we employed amplicon sequencing of the 16S rRNA gene using DNA extracted from breast implant capsule samples. These cultivation independent analyses revealed that capsule associated biofilms are more diverse than canonical single-species infections, but have relatively low diversity (~ <100 species) compared to many host-associated microbial communities. In addition to taxa commonly associated with capsular contracture, the biofilms analyzed comprised a number of taxa that escaped detection in cultivation-dependent work. We have also isolated several key taxa identified through the culture-independent analyses. Together our analyses reveal that capsule biofilms are more diverse than cultivation studies suggest and can be heterogeneous within an individual capsule, between breasts of the same patient, across similar implant types, and over a range in severity of contracture. The complex nature of these communities requires further study across a broader suite of patients in addition to higher resolution analyses including metagenomics to better assess the fundamental role of microorganisms in capsular contracture. |
format | Online Article Text |
id | pubmed-8031459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-80314592021-04-14 Microbial community compositions in breast implant biofilms associated with contracted capsules Crowe, Sean A. Simister, Rachel L. Spence, Jenifer S. Kenward, Paul A. Van Slyke, Aaron C. Lennox, Peter Carr, Nick PLoS One Research Article Subclinical bacterial infections (biofilms) are strongly implicated in breast augmentation failure due to capsular contracture, and while these infections are generally ascribed to common skin commensals, this remains largely unsubstantiated through robust cultivation independent analyses. To determine capsule biofilm microbial community compositions, we employed amplicon sequencing of the 16S rRNA gene using DNA extracted from breast implant capsule samples. These cultivation independent analyses revealed that capsule associated biofilms are more diverse than canonical single-species infections, but have relatively low diversity (~ <100 species) compared to many host-associated microbial communities. In addition to taxa commonly associated with capsular contracture, the biofilms analyzed comprised a number of taxa that escaped detection in cultivation-dependent work. We have also isolated several key taxa identified through the culture-independent analyses. Together our analyses reveal that capsule biofilms are more diverse than cultivation studies suggest and can be heterogeneous within an individual capsule, between breasts of the same patient, across similar implant types, and over a range in severity of contracture. The complex nature of these communities requires further study across a broader suite of patients in addition to higher resolution analyses including metagenomics to better assess the fundamental role of microorganisms in capsular contracture. Public Library of Science 2021-04-08 /pmc/articles/PMC8031459/ /pubmed/33831065 http://dx.doi.org/10.1371/journal.pone.0249261 Text en © 2021 Crowe et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Crowe, Sean A. Simister, Rachel L. Spence, Jenifer S. Kenward, Paul A. Van Slyke, Aaron C. Lennox, Peter Carr, Nick Microbial community compositions in breast implant biofilms associated with contracted capsules |
title | Microbial community compositions in breast implant biofilms associated with contracted capsules |
title_full | Microbial community compositions in breast implant biofilms associated with contracted capsules |
title_fullStr | Microbial community compositions in breast implant biofilms associated with contracted capsules |
title_full_unstemmed | Microbial community compositions in breast implant biofilms associated with contracted capsules |
title_short | Microbial community compositions in breast implant biofilms associated with contracted capsules |
title_sort | microbial community compositions in breast implant biofilms associated with contracted capsules |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8031459/ https://www.ncbi.nlm.nih.gov/pubmed/33831065 http://dx.doi.org/10.1371/journal.pone.0249261 |
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