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A mobile genetic element increases bacterial host fitness by manipulating development

Horizontal gene transfer is a major force in bacterial evolution. Mobile genetic elements are responsible for much of horizontal gene transfer and also carry beneficial cargo genes. Uncovering strategies used by mobile genetic elements to benefit host cells is crucial for understanding their stabili...

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Autores principales: Jones, Joshua M, Grinberg, Ilana, Eldar, Avigdor, Grossman, Alan D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032392/
https://www.ncbi.nlm.nih.gov/pubmed/33655883
http://dx.doi.org/10.7554/eLife.65924
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author Jones, Joshua M
Grinberg, Ilana
Eldar, Avigdor
Grossman, Alan D
author_facet Jones, Joshua M
Grinberg, Ilana
Eldar, Avigdor
Grossman, Alan D
author_sort Jones, Joshua M
collection PubMed
description Horizontal gene transfer is a major force in bacterial evolution. Mobile genetic elements are responsible for much of horizontal gene transfer and also carry beneficial cargo genes. Uncovering strategies used by mobile genetic elements to benefit host cells is crucial for understanding their stability and spread in populations. We describe a benefit that ICEBs1, an integrative and conjugative element of Bacillus subtilis, provides to its host cells. Activation of ICEBs1 conferred a frequency-dependent selective advantage to host cells during two different developmental processes: biofilm formation and sporulation. These benefits were due to inhibition of biofilm-associated gene expression and delayed sporulation by ICEBs1-containing cells, enabling them to exploit their neighbors and grow more prior to development. A single ICEBs1 gene, devI (formerly ydcO), was both necessary and sufficient for inhibition of development. Manipulation of host developmental programs allows ICEBs1 to increase host fitness, thereby increasing propagation of the element.
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spelling pubmed-80323922021-04-12 A mobile genetic element increases bacterial host fitness by manipulating development Jones, Joshua M Grinberg, Ilana Eldar, Avigdor Grossman, Alan D eLife Microbiology and Infectious Disease Horizontal gene transfer is a major force in bacterial evolution. Mobile genetic elements are responsible for much of horizontal gene transfer and also carry beneficial cargo genes. Uncovering strategies used by mobile genetic elements to benefit host cells is crucial for understanding their stability and spread in populations. We describe a benefit that ICEBs1, an integrative and conjugative element of Bacillus subtilis, provides to its host cells. Activation of ICEBs1 conferred a frequency-dependent selective advantage to host cells during two different developmental processes: biofilm formation and sporulation. These benefits were due to inhibition of biofilm-associated gene expression and delayed sporulation by ICEBs1-containing cells, enabling them to exploit their neighbors and grow more prior to development. A single ICEBs1 gene, devI (formerly ydcO), was both necessary and sufficient for inhibition of development. Manipulation of host developmental programs allows ICEBs1 to increase host fitness, thereby increasing propagation of the element. eLife Sciences Publications, Ltd 2021-03-03 /pmc/articles/PMC8032392/ /pubmed/33655883 http://dx.doi.org/10.7554/eLife.65924 Text en © 2021, Jones et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Jones, Joshua M
Grinberg, Ilana
Eldar, Avigdor
Grossman, Alan D
A mobile genetic element increases bacterial host fitness by manipulating development
title A mobile genetic element increases bacterial host fitness by manipulating development
title_full A mobile genetic element increases bacterial host fitness by manipulating development
title_fullStr A mobile genetic element increases bacterial host fitness by manipulating development
title_full_unstemmed A mobile genetic element increases bacterial host fitness by manipulating development
title_short A mobile genetic element increases bacterial host fitness by manipulating development
title_sort mobile genetic element increases bacterial host fitness by manipulating development
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8032392/
https://www.ncbi.nlm.nih.gov/pubmed/33655883
http://dx.doi.org/10.7554/eLife.65924
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